cell culture - (Nov/24/2006 )
do different sizes of flask will develop different growth curve of plant cell suspension culture?
-opliang01-
Are those in agitation? its shouldnt affect as long the parameters of aeration remain constant from one flask to another
QUOTE (opliang01 @ Nov 24 2006, 10:35 AM)
do different sizes of flask will develop different growth curve of plant cell suspension culture?
-tertu-
QUOTE (opliang01 @ Nov 24 2006, 02:35 AM)
do different sizes of flask will develop different growth curve of plant cell suspension culture?
They are agigated. I ve tried out 250ml, 500ml and 1L flasks. The ratio of inoculum size:flask is 0.5g/30ml:100ml flask. The optimum day for harvesting cell culture is totally different so i suspect that the sizes of flask or medium volumn is the factor, is that true?
-opliang01-