what is the function of the morpholinopropanesulfonic acid? - cell labeling (Sep/03/2006 )

i saw some people could use the morpholinopropanesulfonic acid to resuspend the cell pellets for doing further cell labeling, i would want to know what is the function of the morpholinopropanesulfonic acid?

Thanks

-peggybee1-

This is the fancy name for MOPS, which is one of the "Good" buffers. Its function is to hold the pH constant. See:

Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry 1966 Feb; 5(2) 467-77. pmid:5942950.

Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol 1972; 24 53-68. pmid:4206745.

Blanchard JS. Buffers for enzymes. Methods Enzymol 1984; 104 404-14. pmid:6717292.

-phage434-

QUOTE (phage434 @ Sep 3 2006, 10:27 AM)

THanks for yr idea, but how can i make the MOPS solution?

-peggybee1-

QUOTE

...but how can i make the MOPS solution?

Dissolve morpholinopropanesulfonic acid (MOPS) in water?

I don't mean to be flippant, but that's really all there is to it, except to achieve the correct molarity and adjust the pH...

-HomeBrew-

Except to perhaps add that often buffer compounds do not dissolve until they are neutralized. You might wait a very long time if you didn't add base to MOPS in water. MOPS should not be autoclaved, but rather filter sterilized. It is also light sensitive, and should be protected from light. When it turns dark yellow it should be discarded.

-phage434-