Looking for a good cell line to study beta-catenin activity using transient tran - Please recommend a cell line that is good for transient transfection o (Nov/12/2007 )

Hi all,

I am interested in beta-catenin signaling and I plan to transiently co-transfect TOP-flash reporter gene and a kinase of my interest ("A") to see if A inhibits transcriptional activity of beta-catenin (endogenous levels).

So can anyone recommend a cell line that has........

1. endogenously high levels of beta-catenin

2. no mutations in beta-catenin signaling (ie, no constitutively active beta-catenin mutant/no mutations in inhibitory functions of APC)

3. good efficiency for transient transfection (high expression of transgene)

I've already checked 293 cells in the literatures but the authors always transfected beta-cateniin construct as well probably because this cell line has low expression levels of endogenous beta-catenin.

Thank you all in advance.

I don't know any specific cell lines but I would look up the expression profile of beta-catenin and choose a cell line based on that. If you can find the papers that originally characterised the gene and others measuring expression levels of beta-catenin in the various tissues then that might be a good starting point for choosing a cell line. Choose a human cell line if you are studying human beta-catenin. That keeps the study more relevant.

Hi everybody,

I am on a project where we must determine the osteoblast proliferation on metalic surfaces. I have to choose the better type of cells for this. I had read a lot of papers, but I still don't know what kind of cells do I must to choose. I know that MG63 cells or hFOB 1.19, are currently used for this kind of project.

Is there anyone who can help me on this? I really appreciate it.

Thanks in advance.

Claudia