Proteinase k treatment of cells - (Feb/21/2007 )

hi-

what would be a good concentration of proteinase k to use for suspension cells w/o completely killing them? also, how long of an incubation at 37 deg?

1 hr at 37 and then 10min at 65. try this

hi...

thanks for your answer about incubation times...

but what do you think might be a proper concentration of proteinase K?

i'd like to "shave" my cells w/o killing them....