Raji cell subculture - Culturing Raji cells: DMEM or RPMI? (Jan/18/2006 )

Hi there!!

I have a question regarding the culturing of Raji cells (B-cell lymphoma). Following one scientists recommendation, I'm currently culturing them in DMEM (high glucose, without pyruvate, with glutamine) supplemented with 10% FCS. However, all the paperwork and cell banking places suggest RPMI (+10% FCS) as the optimal growth medium. I've been growing them in DMEM and they're growing and viable, but they're just not fast growing - I've been told they grow like weeds, but at the moment they're lagging a bit...

Does anyone have any experience in culturing Raji cells, and what medium might be the most suitable?!

Thanks, guys!

Gayle

Hi,

we actually culture the Raji cell line in RPMI 1640 supplemented with 10% FBS and genta, nothing else, in 5% CO2 incubator in humidified atmosphere; the cells grow very well in these conditions

Sébastien_

Thanks, Sebastien! That's really helpful! We may have to convert to the RPMI, then, if they don't perk up!!

Cheers,

Gx

Oooooh, Sebastien, just one more thought: does your RPMI medium contain any glutamine?? You didn't comment on it in your reply; I've been suggested to supplement Raji cell medium with stable forms of glutamine (the dipeptide L-ala-L-Gln), even if the medium comtains Gln already. Is this absolutely necessary, or can I proceed without this supplement?!

Thanks,

Gayle

oh yes, you are right!
our RPMI medium effectively contains ultraglutamine (the dipeptide L-Ala-L-Gln), so we don't add glutamine any more...
but i don't know if it's realy necessary, i haven't test RPMI w/o glutamine nor ultraglutamine...
i'm sorry

Sébastien_