T cell isolation from rat wholeblood - (May/07/2001 )

Hi, does anyone has a modified protocol for isolation of T lymphocytes from rat (not human)blood using fycoll gradient? Thanks.

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i also want to know.
do you know now?

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hi marina,

i think you have to use lympholyte-mammal (cedarlanelabs.com) to isolate lymphocytes from rat blood but have a look at cedarlanes homepage. if you want to isolate subpopulations from the blood i think magnetic beads from miltenyi is exactly what you need.
flausch

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