CHO-S cell - (Dec/26/2007 )

Dear Sir/Madam,

I have an important question about CHO-S cell (cat No.11619-012 Gibco) derived from Invitrogen. We tried to grow them in monolayer but failed. The media we used was F12 Nutrient mixtures (Ham) , cat No 11765-054 Gibco) + . Do you have any suggestion?
We also used DMEM (4500mg/L D-glucose, L-glutamine and 110 mg/L sodium pyruvate , cat No 11995-065 Gibco) + 10% FBS which make the cells adhere to the plate but they grow very poorly.

Your kind suggestion of the appropriate media or adaptation of the above media will be greatly appreciated. We look forward to hearing from you as soon as your convenience.

Regards,

It is for suspension culture. You can use regular CHO for adherent culture. The DMEM medium seems to be wrong one to use. F12 (Ham) is the right medium.