ethanol/IPA kill cell - (Jul/12/2006 )

I am going to test my calcein AM and propidium iodide probe.
Does anyone have protocol for killing cells with IPA/ethanol?

Never done this. But it would be a simple experiment to design on cells in multiwell plates. You just add varied amt of ethanol to it.

To address your previous question on agents that kill cells slowly. You could test this live/dead assay system in a cell transfection experiment using lipofectamine2000/DNA.