Freezing down cell lysate - (Nov/16/2004 )

Hi,

Does anyone know whether its possible to freeze down whole cell lysates (Produced using the Nonidet P-40 detergent lysis buffer) for a time?
I am going on holiday for a long weekend and have transfected cells which will be ready on the Friday. I was planning on freezing the lysate and immunoprecipitating stuff on the Tuesday.

Has anyone tried this before and if so did it work?

Cheers

Padge5000

I've personnaly nerver done that, but I did immunoprecipitate over weekend (Friday to Monday) and it worked fine (I usually IP overnight). Unless you have precise IP time to respect, you could IP o/we.

Other than that, I wouldn't hesitate much freezing my lysate o/we and IP the week after... not much moves at -20°C!!

Simon

Generally when you freeze whole cell lysates (in -20 or even better in -70 Celsius degrees) it can be stored in a freezer up to one month, but it is not allowed to refreeze - you can only freeze and thaw a sample one time.