Which part of the kidney are COS-7 cells from? - (Dec/04/2008 )

Because is essential for my experiments to know if the protein I'm looking for (located in renal tube and glomerul) is expressed in COS-7 cells I want to know if these cells were obtained from epitelial cells of the renal tube or from other part of the kidney...
Anyone knows for sure?

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If you look at this ATCC product description, it says the morphology is fibroblast-like. So, less likely that they were derived from epithelial cells.

But I can not be sure, primary cells may change in character

1. at the time of line establishment,
2. transformation, and
3. according to culture conditions and
4. number of passages.

You can call ATCC, or primarily presume this not to be epithelial cells, at least in their current character, irrespective of origin. Even if this was epithelial origin, because of the fibroblast character now, it may not express your protein.

Edit: looking at their photograph, it is obviously not fibroblastic, but fibroblast-like. And to meit looks like from this low resolution photograph that it also has epithelial-like clustered morphology at high density. So, don't know!

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