count cell number - (May/14/2008 )

Hy boys!
How can i count the number of EUKARYOTIC cells in colture, using a spectrophotometer setted to 230, 260, 280, 320, 595 and 600 nm?
Please help me...
Thanks

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Generally you just use hemocytometer, but in batch cultures, people do spectrophotometry. The OD is 650nm.
..

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As cellcounter said, people generally use a hemocytometer to count cells as part of passaging the cell lines. If you're trying to quantify the number of cells at the end of an assay, MTT is a common method. It really depends on what you're trying to count in what situations.

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Ok, i'll try with OD 650 nm!!
Thanks!

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