High-throughput screening of suspension cell lines - (Jul/30/2008 )

Hi all,
I'm looking for assays I could use in a high-throughput setting for suspension cell lines on either a 96- or 384-well plate format, where we'd be reading cell viability 72 hrs after the addition of drugs.

Ideally, I'd like to avoid the 'live cell' assays (MTS etc) as finding a single set of conditions to suit all lines will be difficult.

Does anyone know of any assays where the cells can be fixed first and then read?

Thanks

U

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Maybe you have a look on the page of roche applied science. we order our cell proliferation and apoptosis assays there. they have a good choice of assay panel. also for 96 and 384 well plates.
maybe the wst-1 assay or a BrdU-ELISA will fit for you?
if you had adherent cells i would be curious if somebody already tried the xCelligence system which unfortunately does not working with suspension cells...

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Thanks, but I'm really looking for an assay where I can fix the cells beforehand. My experience with live cell assays where some metabolic step is required to generate the signal, is that there is a lot of variability between cell lines regarding the incubation time in the substrate. Likewise, ELISA's not going to be suitable for a high-thorughput platform.

Any other ideas?

U

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