Preparation of competent cells of pichia - (Feb/28/2005 )

have bought the multi-copy pichia expression kit of . But my transformation of pichia failed for many times. Every time I discovered that GS115 strain grow together to form a lot of small particles wihch contain 10-30 cells because of its budding. Did the pseudohyphae influence the efficiency of transformation? How can I prevent this phenomenon?

hi canoeli!
I know this topic is very important to you, but for readablity reasons, please avoid triple posting of the same question....

I think the people from the "protein expression in Pichia pastoris" thread can help you best:

http://www.protocol-online.org/forums/inde...?showtopic=3277

you posted your question there already, so I'm closing this thread now....