primary sertoli cell transfection - (Nov/28/2007 )
Hi all,
I am trying transfection on primary cultures of sertoli cells using transfection reagent. but the efficiency i am getting is very low around 2-3%... has anyone done it before? any idea of transfection on primary cell culture is welcome
-chirpy-
QUOTE (chirpy @ Nov 28 2007, 11:04 AM)
Hi all,
I am trying transfection on primary cultures of sertoli cells using transfection reagent. but the efficiency i am getting is very low around 2-3%... has anyone done it before? any idea of transfection on primary cell culture is welcome
I am trying transfection on primary cultures of sertoli cells using transfection reagent. but the efficiency i am getting is very low around 2-3%... has anyone done it before? any idea of transfection on primary cell culture is welcome
What method are you using?
-Clare-
QUOTE (Clare @ Nov 28 2007, 05:15 PM)
QUOTE (chirpy @ Nov 28 2007, 11:04 AM)
Hi all,
I am trying transfection on primary cultures of sertoli cells using transfection reagent. but the efficiency i am getting is very low around 2-3%... has anyone done it before? any idea of transfection on primary cell culture is welcome
I am trying transfection on primary cultures of sertoli cells using transfection reagent. but the efficiency i am getting is very low around 2-3%... has anyone done it before? any idea of transfection on primary cell culture is welcome
What method are you using?
I am using a transfection reagent EXPRESS IN TRANSFECTION. actually i need to know if someone before has tried sertoli cell transfection and the method which have been used.
-chirpy-