estimating number of cells to seed - (Apr/09/2007 )

Hi,

how do I roughly estimate the number of cells to seed without using a hemocytometer in a 6 well plate from a T75 so that I may reach abt 60% confluency the nxt day for transfection.

Thnks

Have a nice day

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Hi,
First you have tho check from the website of your plate company how much bigger the area of T75 of flask is than the 6 well. (The surface area of each plate is given). Lets assume; it is 5 times more. Than you should know how long it takes your cells to mutliply. Lets say in 24 hours your cells duplicate. Now, if you were to seed them in the T-75 flask for the next day to be 60% confluent you would seed the 30% of the cells from a 100% confluent T-70 flask. Since the 6 well is 5 times smaller, if you want to seed them there you should take 6% of the cells from the T-70 flask. That is how I do at least.
Hope this helps. Good Luck!

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Hi,

thanks a lot. I understand and will try it out
have a nice day!

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