DNA Sonication of quiescent cells - (Jun/14/2005 )

Hello !

Till now, i worked with activated primary T cells and didn't get any trouble to get efficient sonication (cuphorn). But i'd like to make a comparaison with
primary T cells left unstimulated... my problem is that i don't manage to get an efficient sonication... my samples looks as if DNA was not sonicated (sticky, gluwy) even after reversion of crossling and prot K digestion.

Does anyone observed such a phenomen ? I guess it's logical, since chromatin is more compact in such cells... how do you solve the problem !

Thanks !

VICART have a look at the following link
Sonication and other woes

that is pinned to this forum. I am sure you will get some tips there.

Good luck!

Nick