cancer cell culture - protocols on cancer cell culture (Nov/21/2004 )

i am a beginner in the field of cancer research, i am now culturing few cancer cells like aml-2 cells,hep g2 cells,t47d cells,a549 cells and 124 cells,,can anybody please send me the information on the culture of cancer cells????or u can also help me finding out the websites related to this field,,i have tried but i am not satisfied. thanking u in advance.


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Dear Puspa

All these cell lines can b routinely maintained in DMEM supplemented with 10% FBS or FCS. After the cells have become confluent u can subculture them in 1:3 to !:5 by tripsinization .

U can have more info on these cell lines at the official websites for atcc and ecacc. U can also read the bible of cell culture "Culture od Animal Cells" by R. Ian Freshney.

Good Luck

see this web http://www.dxy.cn/bbs/post/page?bid=66&sty=1&age=0

Hi,

I am also new on this research area. And I try to growth HepG2 cells. I am Using RPMI 1640 Media with L-glutamine + %10 FBS. They growth very slowly and they have attach problem on the surface. It takes long time.

I have a question concerning cell harvesting. I have commertialy avaible Trypsin-Edta solution in normal saline (freez). Should I use it directly or dilute it 1:250.

Thank you in advance for your advices.

Gorkem

I just thawed some HepG2 cells myself and I am using Eagle's Minimal Essential Medium with Earle's BSS and L-glutamine + 10% FBS and they seem to be growing rather quickly and are attaching fairly well. You may want to check what type of flask or plate you are using (make sure it's tissue culture grade ... that can affect how well the cells attach).

Another really good resource to look for information on your cell lines is through ATCC (American Type Culture Collection) they have information on thawing the cell lines, subculturing them (including subcultivation ratios), reccomended media for the cell lines, and sometimes info on the behavior of the cell lines. Of course they are also where you buy cell lines and products for your cell lines, but they do have good basic info.

http://www.atcc.org/common/catalog/cellBio...iologyIndex.cfm

I hope this attached file would be helpfull for all of us
I am waiting for ur respond

Check out the proper growth and storage conditions at the ATCC (useless search engine on their site, but google ATCC and your cell line usually works). Media are not transferable between cell lines and the CO₂ % makes quite a difference to who cells grow. For instance T47D should be grown in RPMI 1640 at 5% CO₂, but A549 should be grown in DMEM at 10%.