What the chance to get live cells? - (Jun/17/2005 )

A foreign lab will kindly ship me a mammaian cell line. But the cells they have were in the -80 freezer instead of nitrogen for the many years. Does anyone have idea what the chance I can still raise them?

There is still chance to get living cells. I sometimes revive cells stored in -80 freezer.

Cells can be stored @ -80 for years without loss of cell viability.

hi
according to scientists' community, the lower temperature for living activity is -70°C. Hence -80 or -196 is not that important according to viability. But in for preservative conditions nitrogen is more suitable, due to the lack of great changes in temperature, and it's more practical.

Sweet.
Someone suggest the cells can only only be stored in -80 for at most 1 year. But maybe it is due to the great changes in tempertures of -80 refrigerator.

macrosky,

To make sure the cells have the best chance of surviving, when you thaw them, plate them into a smaller vessel. When I know there are not many cells in a vial, or they have been subjected to some sort of hazard or poor freezing method, I thaw then into a T25 flask, that way there is less media that needs to be conditioned by the cells and they will grow better closer together in the beginning.

Good Luck,

Beverly

Oh, and thaw then right away when you get them. Cells that have been on dry ice are not going to survive for very long in the freezer, regardless if it is liquid nitrogen or -80C.

Can someone give me a protcol for freezing MCF-7 cells in -80 C
thanks
p.

Pria,

I just posted a rather lengthy (sorry) description of my cell freezing opinions in the pinned topic "freezing and thawing of cells". My post is located at the end on page three.

I use this protocol for every cell line I have ever cultured, including the MCF-7. As long as your culture is in log phase, you freeze lots of cells, and you cool the cells appropriately, it should work out just fine.

If you have more specific questions about the MCF-7, just let me know.

Beverly

Beverly,
Thanks a lot!
I will let u know the result

Thanks. Beverly..
sorry I was just feeling a bit lazy to put in a search and sift through the answers.
p..