DOES DMSO CHANGE THE CELL? - (Sep/23/2007 )

HAS ANYONE FROZEN PBMC, OR OTHER CELL TYPES, USING 10% DMSO AND THEN CULTURED THEM? FIND ANY CHANGES IN THE CELL'S CHEMICAL COMPOSITION THAT EFFECTED CELLULAR RESPONSES BY ACTIVATING OR INHIBITING PATHWAYS OR CHEMICAL PRODUCTION - - ANY CHANGES IN THE MEMBRANE PHOSPHOLIPIDS, INTERLEUKIN RELEASE, CA2 CHELATION, NITRIC OXIDE PRODUCTION, KINASE INHIBITION.... I APPRECIATE ANY HELP!!!

It may affect many celluar processes. Search PubMed using this phrase "dmso"[title] you will find out.

We have observed that using DMSO increases proliferation of primary human epithelial cells!