Transfection of cryopreservative cells - (May/27/2007 )

Hi I started cryopreservative cells and I wonder how many passages I need to do before I can do the transfection experiment on these cells.

Thanks

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transfection efficiency depends on various things among them, the type of cells; oncogenic cell lines are more less better to transfect than non-oncogenic immortal cells or primary cells;

you may offer which types of cells you use to discuss in more details...

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You can do transfection after one pass.

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I thaw cell, passage it once, then transfect them.

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