Words, Words, Words - it's only words (Jul/21/2009 )
logarithm
DNA in RNA!! what method do u use??
algorithm
TRIzol..........had 16 samples, of which 2 hv contam, since i m gtng amplif. in -RT reaction as well....
Flow chart
DNA carry over with Trizol after a DNAse ttmt! I never had this problem!!
a. what was the starting material, tissue/cells ?
b. homogenization method - mortar pestle/ bead mill/polytron?
fall apart
from cells....just collected the cells n resuspended in trizol........processed it further acc. to the protocol........thats how all the other samples were done........rest all are fine except these 3.....
hey, i m going for dinner....c u in 15 min.....
am alone????
right in front of u
hey, if all the cells were processed together and you see it only in 3, it sounds strange! .
eye witness
why else do you think i am bugged???? anyway, i hv the duplicates, i'll chk in those also.........
nyway, howz ur day been??? apart from the yeast...
crime
hmm....sometimes shit happens and u don't have a clue what went wrong!
I you have an aliquot before DNase treatment, why don't you run a comparison? maybe b'coz of a poor DNase ttmt!
The rest was good for me...finished RNA isolation and ran the gel...looks good! I was spoiled by bioanalyzer..Now back to painful RNase free gels
criminal
arrey nahin yaar, i used the same aliquot of DNase....all the samples on the same day....
no, i hvn't preserved the pre-treatment one.....hope the other ones r fine..............u know what, these are small things, but can be really irritating at times............how i wish i cud shout at that silly DNA, and give it a piece of my mind..........
uh oh! u still hv work left??
minimal
hmm....the unexplainable phenomenon...happens sometimes..cant help it
dont break ur head thinking of that...go home and take rest...start sfresh tomorrow..maybe u'll figure it out later! I am done with the days work..leaving now..will be online in another 15 min...see u if u're still around else, good night!!
wages
yeah....