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Vector Related Discussions
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Plasmid pCP20 problem - (reply: 1)
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Creating empty vector - (reply: 5)
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cloning vector and restriction mapping - (reply: 2)
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software to replace Vector NTI and a simple cloning problem - (reply: 3)
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Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3)
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plasmid - (reply: 2)
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no expression in pET 21 vector - (reply: 2)
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DNA purification - Touble with isolating PCGP plasmid (reply: 3)
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Good vector for genomic library - (reply: 2)
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NO expression in pKK223-3 vector in DH10B Why ? - in DH10B NO expression in pKK223-3 vector in DH10B Why ? (reply: 2)
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[Help] New vector construct problem - (reply: 4)
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contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
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why use beta galactocidase as a positive control in co-transfection - why not an antibiotic resistant plasmid (reply: 13)
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Does plasmid DNA mutate over time? - (reply: 2)
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suitable vectors for 3' UTR Luciferase? - (reply: 2)
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miRNA expression vector - (reply: 3)
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Plasmid DNA storage - (reply: 1)
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Vector NTI no longer free - (reply: 7)
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co-transfgection - antibiotic plasmid instead of B galactocidase (reply: 2)
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directional cloning problem - two-digested vector and insert - ligate - no colonies?? (reply: 2)
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plasmid isolation problem HELP! - (reply: 2)
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Cloning into pGBKT7 vector - (reply: 4)
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pRARE2 vector sequence - (reply: 4)
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plasmid preparation - (reply: 10)
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Plasmid DNA concentration - (reply: 2)
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Plasmid isolation from bioluminescence bacteria - Alkaline lysis (reply: 5)
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pGL-3 vector transformation - (reply: 5)
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Phosphatasing to prevent vector reclosures - (reply: 1)
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A problem with plasmid DNA extraction - (reply: 6)
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searching for a piece of plasmid information - (reply: 1)
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IPTG induction in phage display techniques - (reply: 2)
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The vector became smaller - (reply: 4)
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eukaryotic inducible vector with GFP-tag fusion? - (reply: 3)
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DsRed stable transfections? - problems with Stoplight vector in Flip-in cells (reply: 4)
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phrGFP-1 - searching for vector sequence - (reply: 6)
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Working with TOPO II cloning vector - (reply: 2)
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Novagen pet 32 Ek/LIC vector - (reply: 2)
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Easiest cloning strategy for deleting part of plasmid using adapters? - (reply: 1)
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Plasmid Isolation - (reply: 1)
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Problem with GST tagged baculovirus protein - (reply: 2)
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non dephosphorylated vector gave white colonies - (reply: 2)
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plasmid isolation and gel - (reply: 1)
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How to transform with topo vector - (reply: 6)
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pET-16b vector in DH5alpha E. coli - (reply: 7)
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Using pGEMT Vectors - (reply: 4)
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Purification of RF DNA from phage prep - Need to get rid of ssDNA contamination (reply: 2)
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Cloning of plasmid - (reply: 3)
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Expression vector and Stop codon consideration - (reply: 2)
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No DNA in 1 of 6 plasmid preps (Qiagen - Midi) - (reply: 2)
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transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
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Two CMVmin in same plasmid? - (reply: 2)
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Who has a good protocol to cut a plasmid? - (reply: 1)
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Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate? (reply: 8)
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Is there any way to remove chromosomal DNA from plasmid DNA? - (reply: 6)
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phage titer problem - (reply: 1)
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How to construct miRNA expression vector - (reply: 1)
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Large MSCV Retroviral Vector Cloning --- Help Needed! - (reply: 3)
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how to get rid of a plasmid? - (reply: 7)
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Erroneous PCR product due to plasmid DNA contamination - (reply: 2)
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vector seq & Multiple cloning site design. - (reply: 2)
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Single Vector multiple promoters? - (reply: 8)
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Medical significance of plasmids - (reply: 3)
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Problem of Plasmid Preparation - (reply: 7)
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Attaching DNA bases - Creating primers and plasmids? (reply: 3)
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How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
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how many plasmids can a competent bacteria take - (reply: 9)
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Insert/vector ratio for ligation - (reply: 3)
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Baculovirus expression problem - Mixture of Bacmid DNA (reply: 2)
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Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
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how to clone my insert DNA into vector? - (reply: 11)
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methylation of plasmids - (reply: 1)
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plasmid digestion gel - (reply: 4)
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Rearrangement in plasmid? - (reply: 2)
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restriction digestion of plasmid - (reply: 8)
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methylated plasmids in culture - (reply: 1)
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How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
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Plasmid transfection - should amount be adjusted according to insert size? - (reply: 4)
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plasmid miniprep - (reply: 1)
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cohesive-end ligation - cloning a fusion protein to a vector (reply: 1)
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Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
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TAT-GFP expression from pRSET vector - (reply: 1)
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plasmid transfection - (reply: 1)
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Expression Vector sequencing problem - (reply: 6)
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Expression Vector sequencing problem - (reply: 1)
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Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
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Cloning with methylated vector - (reply: 2)
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Plasmid Prep Problem : gDNA - bacterial genomic DNA contamination in a plasmid minipreparation (reply: 3)
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Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
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Using IRES in microRNA expression vector - (reply: 4)
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Any PBI121 Vector Information ? - Map, Sequence etc. (reply: 2)
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how to check the insertion of a gene fragment into plasmid - (reply: 2)
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Problems during plasmid prep in cloning - (reply: 7)
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Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
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Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
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Large vector self-ligation in presence of insert (1:1)? - (reply: 3)
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How to find plasmid containing 35S and Nos ? - (reply: 3)
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Need to sequence gel purified vectors exposed to UV? - (reply: 2)
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How to recover old baculovirus stocks - (reply: 1)
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Improving transformation efficiency - large plasmids (reply: 6)
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Plasmid lost during culture - (reply: 19)
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TOP10 competent cell with another vector - (reply: 1)
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Help restriction analysis of plasmid - (reply: 11)
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High Yielding Plasmid Purification (suggestions) - (reply: 1)
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basic questions regarding expression vector work - (reply: 2)
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Can I store plasmid in 4C refrigerator? - (reply: 11)
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BL21 Plasmid extraction? - (reply: 4)
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commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
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Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
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T1 phage infection - can it be due to poor lab practice? (reply: 4)
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How to induce self-ligation of a vector? Self-Circularization? - (reply: 12)
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MIDI's low recovery or vector issues? - (reply: 2)
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plasmid copy number - experiments on how to compare the copy numbers of different copy-numbe (reply: 3)
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Problems subcloning with the pL452 recombineering plasmid - (reply: 2)
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No Ni-NTA binding--internal start codon of NdeI in pET vector? - (reply: 1)
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difference between subculture in incubator and shaker - on preparation of cultures for plasmid dna extraction, is there a diff (reply: 3)
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which part of gene is needed for normal expression in vector - (reply: 2)
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Screening Tranformation Colonies: Funny Gel Pic and no plasmid?!?! - (reply: 10)
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HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
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How to get an individual plasmid out of a plasmid mix in E.coli - (reply: 6)
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upscaling protein production - is type of vector and host strain crucial factors? (reply: 1)
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How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
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High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid (reply: 1)
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Plasmid DNA preparation - two DNA bands in gel (reply: 5)
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Databases of commercially available plasmids - (reply: 2)
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cloning into plasmid (IMPACT kit from NEB) - (reply: 3)
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help with luciefarse reporter assay,:( - p53 reporter vector versus expression plasmid (reply: 1)
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ta cloning vectors - non commercial ta cloning vectors (reply: 1)
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how to extract plasmids leaving bacteria cells intact - (reply: 7)
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Plasmid Shelf Life? - (reply: 1)
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No protein expression in pKK223-3 vector in JM105 - (reply: 3)
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Concepts about the Vector - (reply: 2)
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How to test if a cDNA clone from a plasmid is infectious? - (reply: 4)
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RNAi vector construction - Has anybody done it? (reply: 2)
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plasmid prep - help (reply: 8)
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In vitro Transcription - Do I need to linearize my plasmid ? (reply: 1)
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multiple plasmids in cells - (reply: 1)
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QUestion on subcloning a 80bp product from TOPO vector - (reply: 5)
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TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
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Sequencing result is vector self-ligation while bacterial PCR identification was - (reply: 5)
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plasmid transfection in hepatocytes - (reply: 1)
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Vector replication - (reply: 4)
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RE cloning a 2.3kb fragment into a 4.7kb vector - (reply: 4)
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How can I create a T vector for PCR product cloning - (reply: 2)
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Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
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how to insert a thrombin cleavage site - ....into my vector (reply: 3)
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inducible retro/lentiviral vector - (reply: 5)
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luciferase vector construction - (reply: 3)
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Why there is a artifical intron in some plasmids? - (reply: 4)
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Retroviral vector cloning help - (reply: 6)
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cloning with pGEM T-easy vector - question about the vector. (reply: 2)
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Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
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Fate of plasmid - How to detect plasmid DNA on host chromosome (reply: 7)
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How to search plasmid sequence for restriction sites and resistance genes? - (reply: 4)
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linearized vector purification from TAE agarose gel - (reply: 5)
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help with His-tagged vector construct - (reply: 2)
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Plasmid with two different origins? - (reply: 1)
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EcoR1-Pst1 digest for vector and insert - (reply: 3)
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Vector digestion - (reply: 6)
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sequencing vector DNA - (reply: 4)
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empty vector control - (reply: 4)
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Vector concentration following maxiprep - (reply: 4)
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How about vector sequence in cloning? - (reply: 9)
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How to get rid the restriction sites in a plasmid ? - (reply: 5)
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Expression vector for Pseudomonas - expression of a 10Kb gene in Pseudomonas aeruginosa (reply: 2)
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gene from 4-TOPO to expression vector - (reply: 1)
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PCR product of unlinearized plasmid - (reply: 1)
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MoBio Plasmid Purification and Gel Extraction Kits - Problems with MoBio Kits (reply: 3)
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Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
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Multiple recombinant plasmids in transformed E Coli? - (reply: 2)
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Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (reply: 1)
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Plasmid transformation - (reply: 4)
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Mini plasmid Kit extraction - (reply: 4)
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why a low copy number? - shRNA vector (reply: 3)
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Small fragment sticky-end ligation into EGFP-N1 vector - (reply: 2)
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Miniprep for pGEM vectors - please help - Technical help (reply: 7)
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Uncomplete plasmid after transformation - (reply: 1)
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vector: Klenow + CIP - do they work together?! (reply: 1)
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advice re: shRNA vector sequencing - (reply: 4)
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problem with restriction digest - I have an insert, but no vector.... (reply: 5)
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Help with Maxiprep plasmid low ratio - (reply: 2)
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Annealing plasmid DNA - (reply: 3)
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cyanobacteria inoculation by phage - inoculation of cyanobacteria by phage (reply: 1)
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GFP-Hygromycin vector - (reply: 2)
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Plasmid vs Lentivirus as a transfection vector - Opinions? (reply: 4)
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Fast way to get plasmid for PCR - (reply: 2)
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Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
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how to know the plasmid or phage inside the cell? - (reply: 3)
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Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
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Plasmid coli glycerol stocks @ -20 - will they still be useable? (reply: 4)
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Maxi plasmid preparation - (reply: 3)
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Question about maxi plasmid preparation - (reply: 1)
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Ligation, plasmid rescue problem! - PLasmid transformation after ligation problem (reply: 7)
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DH5 keep kicking out my vector - (reply: 2)
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does low plasmid concentration affect transfection rate? - (reply: 4)
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Plasmid DNA extraction problem - (reply: 3)
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Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
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genomic DNA contamination during plasmid preparation - (reply: 1)
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Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
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plasmid concentration and freezing - (reply: 4)
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puc19 vector - (reply: 1)
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Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (reply: 2)
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pEGFP N1 vector - (reply: 6)
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large plasmid extraction? - how? (reply: 5)
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no plasmid extracted - low copy number - (reply: 2)
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Need help with application of different vectors - (reply: 1)
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cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?? (reply: 2)
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plasmid subcloning - (reply: 1)
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ELISA and phage display - (reply: 7)
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basics about expression vectors - (reply: 2)
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no plasmid after extraction - (reply: 2)
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how can I concentrate my plasmid DNA? - (reply: 3)
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need help to solve problems with plasmid isolation - molecular biology (reply: 6)
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Suggest a name of a bidirectional expression vector - Anyone has idea of this vector? (reply: 1)
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Miniprep plasmid DNA - How clean is it? - (reply: 3)
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P/C/I extraction of plasmid after digestion - (reply: 1)
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PLasmid extraction: good nanodrop reading but NOTHING on the gel! - (reply: 4)
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differential binding of dye to supercoiled and relaxed plasmid DNA - which dye and how? (reply: 5)
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smear when cutting plasmids previously checked - (reply: 4)
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co-transfection of plasmids - ratio - shRNA plamid with plasmid expres'ng target (reply: 8)
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lentiviral vector cloning problem - (reply: 1)
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Frameshift in pET vector? - (reply: 7)
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compare pEGM T easy vector and TOPO TA cloning - (reply: 7)
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miRNA lentiviral vector library - (reply: 1)
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High firefly luc activity in EMPTY pGL3 Basic vector - (reply: 2)
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pGEM vectors - (reply: 3)
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recombinant plasmids lossing - get HELP! (reply: 5)
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pT7SC vector - (reply: 1)
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Problems cloning a tiny insert into a huge vector - (reply: 1)
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Self Circularization Problem with a 6KB vector - self ligation of a 6KB vector (reply: 1)
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Blunt end vector........? - (reply: 2)
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ligating into vector: sequencing trouble - vector sequences before RE but not after (reply: 1)
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How to amplify a phage display library - (reply: 1)
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please recommend some common miRNA expression vectors - (reply: 2)
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Stability of plasmids in recA1 strains - (reply: 4)
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Plasmid problems - low yeild plasmid DNA (reply: 3)
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too much background in vector preps - Vector prep (reply: 6)
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cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
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shotgun cloning: gel shows inserts but only plasmid sequence obtained - (reply: 1)
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Plasmid replication in mammalian cell systems - (reply: 3)
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+and - in plasmid names - (reply: 2)
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PCR of lambda phage - (reply: 1)
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Checking Insert using PCR from plasmid DNA? - (reply: 4)
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transfect from full plasmid and transfect from PCR product - (reply: 5)
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What EDTA does in annealing of DNA and vector? - (reply: 2)
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X-ray scanning of DNA plasmids - (reply: 1)
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cloning in expression vector - (reply: 5)
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Linear plasmid for transfection - where should I cut the plasmid? (reply: 3)
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plasmid DNA maxi prep - (reply: 1)
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Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
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Getting smear problems in plasmid run - (reply: 8)
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Elute DNA from Watmann paper - I received a plasmid in the mail and cant get transfectants (reply: 2)
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co transfection plasmid and siRNA - message from gmgabriel68 (reply: 1)
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identifying genomic DNA contamination during plasmid isolation - (reply: 2)
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E.coli promoterless lacZ vector - (reply: 2)
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How to chose vector and E.coli strain for protein expression - (reply: 2)
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plasmid degradation in tris EDTA - (reply: 10)
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E.coli TOP10 comp cells T1 Phage resistant? - (reply: 1)
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Question about QuikChange II mutagensis method - Can it merge plasmids? (reply: 3)
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Transformation problem with newly ligated plasmids - (reply: 1)
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Low transfection efficiency using PEI - I'm trying to transfect BHK-21, with a large size plasmid (13kb), (reply: 9)
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qPCR and plasmid positive controls - Why use plasmid positive controls? Are they the best? If so, are there (reply: 1)
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pGEX-2T GST fusion vector - (reply: 1)
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Recombinant plasmid lost afer transformation - (reply: 3)
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Lost in plasmid preperation - (reply: 4)
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What is a binary vector? - (reply: 1)
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Low yield plasmid extraction - (reply: 6)
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Plasmid Cleanup - Advice on how to further clean up plasmid created by alkaline lysis an (reply: 4)
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NheI, EcoRI vector double digest - Troubling getting second enzyme to cut (reply: 6)
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Gateway cloning with 3 entry vectors - (reply: 4)
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vector dephosphorylation and ligation - how does it work? (reply: 1)
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Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
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Separate cutting plasmid from uncut? - (reply: 4)
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plasmid DNA purification - which kit should I buy? (reply: 7)
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plasmid prep with protein? contamination - (reply: 3)
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how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
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pEBG vector...Where is it? - Is it possible to purchase this vector? (reply: 3)
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how to store plasmid samples? - (reply: 3)
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Plasmid extraction from filter paper - (reply: 3)
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plasmid prep question - thick white material? (reply: 11)
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help finding eukaryote expression vector - (reply: 2)
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plasmid - (reply: 4)
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No colonies with TOPO vector - (reply: 3)
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Need PLen Plasmid map for stable line set up - (reply: 4)
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Plasmid Ligation does not work-suggestions? - (reply: 6)
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Problem about plasmid constrution - (reply: 2)
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pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
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Why do you need your insert in the vector in both orientations? - Cloning (reply: 4)
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Preference in isolation of plasmid DNA methods? - (reply: 5)
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low plasmid yield - (reply: 8)
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question on pGEM-T-easy vector system - (reply: 3)
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Detection of 10 plasmid copies - detection limit (reply: 5)
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concentration of vector in ligation reaction - (reply: 4)
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Trouble with viral vector --> too many colonies in control plate - Trouble with viral vector --> too many colonies in control plate (reply: 6)
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purposes of uncut plasmid DNA - (reply: 4)
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Protein expression from bacteria which has two plasmids? - Incompatibility of plasmids (reply: 5)
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Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
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how to extract plasmid spotted on paper? - (reply: 7)
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Which vector would you choose for transient expression in CHO cells - (reply: 2)
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cloning of 1 kb fragment in 14 kb vector - (reply: 3)
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adapter liagation into a single cut plasmid? - (reply: 4)
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Restriction digested plasmid - (reply: 2)
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cloning of a big Plasmid - (reply: 3)
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constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
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the ratio of pGL3 promoter( control) vector in Dual luciferase assay - Dula luciferase assay (reply: 1)
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E.coli Compotent Cell Storage and Plasmid Isolation - (reply: 3)
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Blunt Cloning Large Insert/Smaller Vector - (reply: 9)
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phage cDNA library screening with PCR - (reply: 3)
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miRNA expression vector - miRNA expression vector (reply: 4)
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Gel purification of plasmid after each of these steps? - (reply: 1)
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Plasmid pSPL3 - Looking for plasmid pSPL3 (reply: 2)
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why my plasmid became shorter? - (reply: 5)
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plasmid DNA not digested - (reply: 3)
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how the multiple cloning site in cloning vector is create? - (reply: 4)
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plasmid problem - (reply: 3)
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How to mix your plasmid? - (reply: 7)
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transformation competent cells problem - fastbac vectors (reply: 2)
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dephosphorylated vector - (reply: 1)
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one plasmid into each E.coli? - (reply: 13)
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plasmid profile - difficulties with marker and size of plasmids.. (reply: 4)
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need help in interpreting PAGE - weird banding fr phage PAGE (reply: 1)
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Electroporation with a pool of vectors with different inserts - why only one kind of vector maintained in the cells? (reply: 2)
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Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
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why my plasmid is degraded? - (reply: 4)
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Searching for vector sequences - (reply: 2)
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Mutation in plasmid - (reply: 2)
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peptide expression vector - I need to find a vector for bacterial expression of peptides of varyin (reply: 4)
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Is time of ligation insert and vector crucial? - (reply: 4)
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plasmid extrction problem! or more! - (reply: 4)
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HELP! About plasmid transfection in 96 well plate - (reply: 3)
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Retrieve dried vector - (reply: 2)
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propagate pTRE-Tight vector problem - (reply: 6)
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Is this genomic DNA? - or plasmid? (reply: 11)
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Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
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unable to digest my recombinant plasmid! - (reply: 9)
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blunt ending the vector followed by the ligation - (reply: 1)
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Vector for expressing proteins to be secreted - (reply: 3)
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Vector for producing Fc fusion proteins - (reply: 1)
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Can bacteria grow without a plasmid or am i stupid? - or did ampicillin wear out? (reply: 5)
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E. coli transformation issues - old plasmids (reply: 7)
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Large Scale Extraction of Bacteria Plasmid DNA - (reply: 1)
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problem with transformed plasmid's size - (reply: 10)
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Inability to grow plasmid in culture - Molecular cloning (reply: 3)
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Low or high copy plasmid ? - (reply: 2)
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pNUT vector map - (reply: 1)
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plasmid DNA Digestion - (reply: 5)
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plasmid purification - (reply: 3)
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pBinAR vector map - (reply: 1)
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MigR1-GFP vector transfection - Did anyone transfect MigR1 using TransFast (reply: 4)
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Purification of 13 kb plasmid from E. coli - (reply: 4)
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insert:vector ratio for ligation - religation of the vector? (reply: 2)
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pET vector problems (HELP) - pEt 21 a (+) (reply: 3)
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TE diluted plasmids and transfection - (reply: 2)
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HELP! how to retrieve plasmid from glycerol? - (reply: 2)
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Please save me ! - pAdeasy adenovirus vector system (reply: 1)
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Problem with cloning pGEMT easy vector system - (reply: 4)
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pCAMBIA vector map - (reply: 1)
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litic phage contamination during panning! - (reply: 3)
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Molar Ratio of Inser to Vector - (reply: 3)
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What type of PCR to identify a plasmid insert? - (reply: 4)
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Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
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competent cells transform 12 kb plasmid,urgent - (reply: 8)
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Name of double his tag vector - looking for name of company that sells the dual his tagged vector (reply: 3)
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Mammalian protein expresion in bacteria - Which plasmid and strain to use (reply: 7)
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How can I calculate the copy number of plasmid DNA after mini-prep? - (reply: 5)
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reporter vector cotransfection - (reply: 4)
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Can a single restricted vector spontaneously ligate back to a circular DNA? - (reply: 3)
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Problem: Plasmid DNA extraction from S. aureus - (reply: 6)
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integration vector question - do i need to clone a full gene (reply: 1)
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Stable transfection using plasmid with no selection gene - (reply: 2)
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plasmid cloning in E.coli - (reply: 2)
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Restriction of pichia vector pPICZ alpha with Xho1 and Xba1 - (reply: 1)
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what is the plasmid nomenclature: eg.what does it mean by pUC, pCR, and other ve - (reply: 13)
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Low insert and vector concentration - (reply: 7)
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plasmid map drawing - (reply: 2)
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trouble cloning into red vector - (reply: 1)
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miRNA effect on 3'UTR cloned into a reporter vector - (reply: 2)
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pUASp sequence - Need the pUASp vector seq (reply: 1)
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Sequencing a 10kb length of dna in a 12kb plasmid - (reply: 3)
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help with moving an insert to a new vector - (reply: 4)
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Endogenous/Native Plasmid Isolation - (reply: 1)
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pGEX vector - (reply: 4)
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E. coli plasmid info - (reply: 1)
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Plasmid Curing - (reply: 1)
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Plasmid Curing - (reply: 7)
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Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
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digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
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How to add a lethal gene to my plasmid for cloning selection? - (reply: 3)
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How to save my plasmid - I run out of my plasmid and my EColi doesn't grow (reply: 3)
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Problems w/ yield from lentiviral plasmid prep - (reply: 3)
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question:problem during the plasmid isolation - (reply: 7)
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Decreasing plasmid size via (digestion-gel extraction-ligation-transformation) - (reply: 8)
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phage display library - question (reply: 1)
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F1 origin is it mandatory for a plasmid - (reply: 3)
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How to improve 260/280 reading for DNA? - 260/280 for purified digested plasmid is very high. (reply: 2)
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Elementary Question - How do I send plasmid DNA?? (reply: 11)
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Plasmid purification - Purification using Qiagen kit (reply: 2)
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Plasmid stability over a 100%? - Why does ampicillin increase cell viability? (reply: 4)
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HELP! GFP plasmids - GFP plasmids (reply: 4)
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TA-cloning vector contamination - (reply: 3)
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Cosmid and plasmid at the same time - (reply: 1)
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Vector with two promoters - Molecular Biology (reply: 2)
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Transformation-Min plasmid reqd - (reply: 3)
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PLASMID DNA yield - (reply: 3)
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Can CMV promoter be used as transgene vector - (reply: 2)
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plasmid glycerol stock - (reply: 2)
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cloning/ligation problem? (shRNA into vector) - cloning, colonies in neg control (reply: 4)
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Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
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plasmid isolation help urgent.. - (reply: 4)
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Very huge vector for transformation - (reply: 4)
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pGM-T vector problem - (reply: 3)
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pgl3 - empty vector upregulated as much as construct! - (reply: 2)
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How much plasmid for transformation? - (reply: 5)
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No virus titer and MSCV-mir30 vector problem - HELP (reply: 12)
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Plasmid DNA Sequencing with Universal Primers - (reply: 3)
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MSCV mir30 retroviral vector problem - (reply: 2)
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SubCloning binary vector - (reply: 5)
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Phage Display: Not getting antibodies after 3 rounds of panning - (reply: 6)
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question about plasmid transfection - (water solution or buffer solution?) (reply: 3)
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plasmid molar concetration/copy number - (reply: 2)
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how the lentiviral vector transduct into cells? - ask a question (reply: 3)
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When double-transfecting, do both plasmids always go together? - (reply: 4)
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How many plasmid DNA copies per cell? - (reply: 1)
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Need help getting rid of plasmid in E.coli (Rosetta) - (reply: 4)
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Retroviral vector - (reply: 3)
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MSCV LTRmir30 vector sequencing! - (reply: 2)
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plasmid copy number - how one can determine plasmid copy number in E.coli (reply: 2)
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Vector keeps on self ligating - help (reply: 3)
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Plasmid Extraction - Miniprep incident (reply: 3)
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plasmid bands - (reply: 1)
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Heating the vector and insert before ligation - new idea for reducing background colonies (reply: 7)
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Viral / phage promoter prediction - (reply: 1)
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Cloning problems into a big (19 kb) vector - (reply: 10)
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De novo plasmid methylation in eukaryotic cells - (reply: 3)
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Reconstitution of plasmid - (reply: 1)
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Clone 2kb and 5 kb inserts into 6kb vector - Which order and vector:insert ration? (reply: 3)
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what is the mechanism of transformation of a plasmid into a bacterial cell? - (reply: 5)
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about ligation problem - insert and vector size (reply: 9)
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my vector contain ccdB gene in MCS, can i use this as mock control during transf - (reply: 1)
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Modification of Vector - (reply: 1)
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Making more empty vector - (reply: 7)
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M13 phage pfu determination - (reply: 1)
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shRNA lenti vectors - (reply: 5)
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how to purify plasmid and genomic dna - (reply: 3)
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does size matter - how many genes in 1 vector (reply: 10)
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Ligation of a 1.8kb insert into 10.8kb vector - (reply: 4)
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Religated vector despite a "no insert control" - help! - (reply: 2)
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insert to vector ratio - (reply: 3)
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Will self-ligated plasmid run faster than the unligated one? - (reply: 9)
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DNA degradation after plasmid mini preps - (reply: 6)
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Problem transfecting large plasmid into HEK293 cells - (reply: 8)
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Stable Clone establishment using Hygromycin or Puromycin vectors - (reply: 3)
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Vector with many restriction site - (reply: 6)
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Free Plasmid Miniprep Kit - Beta Test (a 42 Dollar value) - (reply: 7)
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how to ligate random oligos to vector to construct a library? - (reply: 6)
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non-lytic phage titer on X-gal plates -urgent help needed - (reply: 1)
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C group Plasmids? - (reply: 2)
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large protein degradation - during baculovirus purification (reply: 2)
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help with recircularizing a blunt-ended vector? - (reply: 6)
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pcDNA3 expression vector is suitable for TNT in vitro? - (reply: 2)
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Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
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Help! my "plasmid" won't cut. - RE digestion problem (reply: 7)
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Help with Cloning into LARGE vector - (reply: 11)
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2 plasmid electroporation. Tips? Advice? - (reply: 5)
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stain genomic and plasmid DNA - FACS analysis (reply: 1)
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about large-scale preparation of empty vector - (reply: 2)
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linear or supercoiled plasmid for creating stable cell line - (reply: 1)
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plasmid stability - (reply: 4)
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RNA transfection VS Plasmid DNA transfection - (reply: 3)
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Has anyone else encounted problems in cloning with PET-22b vector? - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
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the plasmid even smaller than the empty PET-22b vector! - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
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cloning large inserts in large vectors - (reply: 4)
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2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
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A plasmid mobility question. - (reply: 3)
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Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
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shorter vector fragment at the end of the cloning - (reply: 8)
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Interaction of Glucose with plasmid DNA - (reply: 3)
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binary Ti vectors and Agrobacterium strains - (reply: 1)
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Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
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Stable cell trasfection questions... - Integration of plasmid... (reply: 5)
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Plasmid for qPCR standard curve - (reply: 3)
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Plasmid as a PCR template - (reply: 2)
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Difference between Extraction of DNA from chromosomal DNA and plasmid DNA - (reply: 1)
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clonong into a pET 28 vector - (reply: 2)
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Is my plasmid contaminated by RNA? - (reply: 13)
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pCMVneo - need a description, company or plasmid map (reply: 2)
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CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
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why does empty EGFP vector has no fluorescence? :/ - (reply: 5)
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dephosphorylated vector and PCR insert - (reply: 7)
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2 PCR product to be inserted into 1 expression vector - (reply: 2)
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Uncut plasmid DNA - (reply: 6)
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Plasmid DNA Isolation problems, contaminating chromosoma DNA - How to isolate large inserts by miniprep and without chromosomal smearing (reply: 4)
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Can I select stable transfectants with amp-resistant vector? - (reply: 3)
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Isolation of plasmid DNA - alkaline lysis method (reply: 3)
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Isolation of plasmid DNA - chemicals used-why?? (reply: 5)
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genomic DNA contamination in plasmid DNA prep - (reply: 1)
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How long is too long for a TA vector? - (reply: 2)
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plasmids to quantify copies of virus - (reply: 4)
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RACE product cloning in TOPO vector - (reply: 1)
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38bp insert and 6.7kb vector ligation problem - the ligation efficiency is too low (reply: 8)
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annealing oligo to a cut vector - (reply: 3)
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How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
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How to store a linearized Vector? - (reply: 7)
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Which TA vector do you use? - Who do you use, or do you make your own? (reply: 2)
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different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
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T4 phage DNA isolation protocol - (reply: 1)
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Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
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Plasmid CpG methylation after transfection - (reply: 2)
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plasmid purification from mammalian cells? - (reply: 1)
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plasmid purification from mammalian cells? - (reply: 4)
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SDS page and gene cloning - pBS plasmid (reply: 1)
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Cloning of a Large Insert into a Lentiviral Vector - (reply: 5)
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will linearizing plasmid prolong transient transfection ? - (reply: 1)
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OH, another unknown vector (oh, sorry I got it finally) - pLysS (reply: 1)
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Making your own TOPO vector - (reply: 2)
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Request a map of vector pJR-1 - (reply: 1)
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Problems with plasmid transfection into primary keratinocytes - (reply: 3)
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getting rid of undigested vector - (reply: 5)
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Somebody just sent me a plasmid.... - plasmid recovery (reply: 6)
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AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
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Plasmid stability/Cloning problem - (reply: 3)
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reconstituted plasmid DNA solution slightly milky - (reply: 3)
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Problems using gentamicin plasmids - (reply: 6)
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Insert is double the size than vector - (reply: 6)
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Multiple transformation - How many plasmids is it possible to transform at the same time ? (reply: 2)
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too confused about plasmid concentration? - (reply: 4)
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Problems with Plasmid purification - no real precipitation of genomic DNA (reply: 8)
-
Plasmid Sequence for pACT1-GFP - (reply: 4)
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vector problems - (reply: 2)
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which restriction enzyme should I use to digest the vector? - (reply: 3)
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digesting pQE30 vector gives inexplicable results - (reply: 3)
-
Stable transfection of large plasmid into breast cancer cells - (reply: 2)
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same vector but diffirent clones produce bands of different size. - (reply: 4)
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difference between DH5alpha and lamda pair DH5alpha? - (reply: 2)
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Plasmid storage and DNA concentration - (reply: 4)
-
about using the pLentiLOX 3.7 vector in the lentiviral system - (reply: 6)
-
shuttle vector - (reply: 1)
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Plasmid DNA problem - PEG precipitation (reply: 3)
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about MTS assay and plasmid transfection - (reply: 2)
-
where can I get any plasmid with aadA as selective marker gene? - i might offer some money (reply: 1)
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How to make plasmid map? - (reply: 4)
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what is IRES?What is it for? - vector construction (reply: 3)
-
making a vector construct and transfecting in smooth muscle cells - (reply: 6)
-
Will linearized plasmid still have drug resistance? - (reply: 13)
-
Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
-
cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
-
possible contamination of TA cloning vector? - (reply: 4)
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Contamination with TA cloning vector? - (reply: 1)
-
Transformation efficiency of plasmid dna - (reply: 2)
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Supercoil migrates slower than linear plasmid? - (reply: 2)
-
vector for expression of tag on cell surface - (reply: 7)
-
Transformation of a plasmid into E.coli BL21 - problem! - Unexpected bands (reply: 7)
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After double digest my plasmid is 2Kbp shorter! - (reply: 3)
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Express miRNA in vector for gene knockdown - (reply: 4)
-
strange digested vector isolation - (reply: 7)
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Map of MIGR1 retroviral vector needed - (reply: 1)
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why competent cells take up only 1 type of plasmids? - (reply: 7)
-
pXT7 vector map - (reply: 3)
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Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
-
baculovirus expression system - (reply: 3)
-
pGEM T easy vector for cloning - (reply: 29)
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about mutant plasmid construction - (reply: 3)
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How to get suicide plasmid? - (reply: 1)
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Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
-
protein expression in pET-30a vectors - (reply: 2)
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Ligation problem, might be bc/ it's the 5kb insert? - Vector and insert are pretty much same size.. and it's not workin in a ' (reply: 3)
-
who can recommend me a good baculovirus expression system? - (reply: 3)
-
Inserting tag into the vector. - where is IRES? (reply: 11)
-
Regeneration of plasmid preparation columns - Has anyone a protocol? (reply: 1)
-
MIGR1 RETROVIRAL VECTOR - (reply: 2)
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ligation never works even for single cut vector!Why so!Help! - (reply: 7)
-
two plasmids of the same protein with tags on N and C-terminus. - how can I make use of them? (reply: 3)
-
Block-it Plasmid Ligation - (reply: 8)
-
vector for stem cell differentiation - (reply: 2)
-
shRNA Plasmid propagation in Xl1 Blue - (reply: 1)
-
E-coli is eating up my plasmid! - (reply: 9)
-
pECL Eco viral vector and Rat cells - viral receptor present? (reply: 1)
-
inserting shRNA vs full gene into vector ? - (reply: 10)
-
Problems with inverse PCR - Identification of gene trap vector insertion (reply: 3)
-
advice on construct - possible problem with vector (reply: 7)
-
any good method for rapid screening for recombinant plasmids? - (reply: 5)
-
E-coli strain and plasmid incompatibility...? - is there something like that? (reply: 14)
-
vector PCR problem - stuck in experiments... (reply: 2)
-
Can all bacterial plasmids work in plants too? - (reply: 6)
-
Cloning Construction of 9000bp vector with 900bp - (reply: 14)
-
Lambda phage assay - (reply: 2)
-
Mutagenesis Help (plasmid isolation & sequencing stages) - (reply: 2)
-
MIGR1 retroviral vector transfection - (reply: 3)
-
plasmid construction - (reply: 1)
-
Can I still use pGEFP-C1 vector? - (reply: 2)
-
Amplify retroviral vectors doesn't work from miniprep to midiprep - (reply: 6)
-
Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
-
cloning problem with pcr and pegfp vector - (reply: 9)
-
problems with cloning into a pET15b vector - (reply: 2)
-
Religation with single digest vector - (reply: 7)
-
Asking help for purifying plasmids - (reply: 9)
-
Asking help for purifying plasmids - (reply: 3)
-
Added E coli to plasmid by mistake - How to get plasmid back? (reply: 7)
-
vector for express secreted protein in vivo - (reply: 3)
-
VECTOR LIGATION WITH One RE - (reply: 4)
-
ires... - IRES IN VECTORS.. (reply: 3)
-
dimer in vectors - (reply: 2)
-
LOOKING for vector map and sequence of pSVaZ11 - (reply: 1)
-
His-Tag Question (pQE30/31/32 Qiagen Vector) - (reply: 2)
-
PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
-
A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
-
plasmid isolation from a transient transfection - (reply: 2)
-
Should I purify digested vector before ligation? - (reply: 7)
-
Vector NTI's local database - (reply: 2)
-
plasmid DNA running on agarose gel to the opposite direction - (reply: 7)
-
Do I have to purify vector after RE digestion for cloning? - (reply: 17)
-
how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
-
Linealizated plasmid for stable transfection? - is that right? (reply: 1)
-
Does it need cotransfection vector when retrovirus transfected hek293? - (reply: 7)
-
maximum plasmids can be co-transfected into Hela cells - (reply: 4)
-
Plasmid instability - why and how do i check? (reply: 1)
-
Plasmid cotransfection for selection - (reply: 4)
-
Cloning a very small insert into a "giant" vector! - (reply: 8)
-
transformation of competent cells with recombinant plasmids - (reply: 2)
-
plasmids smaller than empty vector - using expensive cells - still not working (reply: 1)
-
checking colonies by vector primers.... - (reply: 7)
-
Gateway vectors - (reply: 1)
-
trouble ligating fusion-PCR into vector - (reply: 6)
-
Any body knows the characters of plasmid pINT007 - (reply: 4)
-
why do supercoiled plasmid transform faster than linear plasmid - (reply: 3)
-
Plasmid isolation - (reply: 2)
-
help in Plasmid 'mini-prep' experiment - (reply: 5)
-
plasmid DNA precipitation - with the use of PEG-MgCl2 (reply: 1)
-
Vector: pIRES - (reply: 4)
-
inducation with lamda CE6 - (reply: 1)
-
insert to vector ratios for ligations - what do y'all do? (reply: 4)
-
endotoxin free plasmid preparation - kits comparable? (reply: 2)
-
cleaning up plasmid preps - (reply: 6)
-
Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
-
Amplification of phage library - (reply: 3)
-
p2T7 Vector Sequence - (reply: 1)
-
Insert 2 single strand DNA into a vector - (reply: 6)
-
disappearing vector after digestion - (reply: 11)
-
Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
-
Removing vector restriction sites - and replacing with new ones (reply: 8)
-
Stuborn band after digestion of plasmid DNA - (reply: 4)
-
How to remove endotoxin? - want a endotoxin-free plasmid for transfection (reply: 5)
-
Vector problems? - (reply: 7)
-
Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
-
Calculating insert/vector ratio - (reply: 7)
-
methylated plasmid - (reply: 3)
-
Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
-
qPCR Standard control - plasmid quantify (reply: 3)
-
PEG precipitation of phage - (reply: 3)
-
plasmid DNA and restrictions enzyme - (reply: 6)
-
Cloning Hell - problems with aquiring insert into vector (reply: 5)
-
Plasmid Purification - Any tricks to increase yield? What could I be doing wrong? (reply: 2)
-
is there a restriction about amount of vector in a ligation? - (reply: 14)
-
To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
-
cloning in a long vector (~10kb) without sequence changes - (reply: 2)
-
does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
-
re-dephosphorylation of vector? - (reply: 3)
-
TA cloning...why is there no plasmid for BLUE colonies? - (reply: 2)
-
plasmid miniprep question - (reply: 4)
-
Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
-
Electrophoresis of Plasmid - (reply: 3)
-
Vector question - (reply: 6)
-
Working with phage( T4 bacteriaphage) - several questions abt phage (reply: 3)
-
pSM2C retrovirus vector from Openbiosystems, is it good? - (reply: 5)
-
Y2H vectors compatible? - (reply: 1)
-
How to choose a suitable phage display system - (reply: 1)
-
What insert:vector ration is best? - (reply: 18)
-
Plasmid map of the clone with APE or DNA Strider - (reply: 2)
-
anyone heard of pEBB vector? - or where to find info? (reply: 4)
-
Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
-
help with transfection of large size plasmid - (reply: 2)
-
isolation of phage dna - isolation of phage dna (reply: 1)
-
plasmid size change after transformation - (reply: 1)
-
Methods for concentrating plasmid DNA - (reply: 23)
-
BAC Vector with SalI in MCS? - (reply: 1)
-
really lost in plasmid ligation - urgent! (reply: 6)
-
Making blunt and stick end vector - (reply: 2)
-
Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
-
why dephosphoration of vector is not recommended? - (reply: 3)
-
how to move for ligation with low conentration of digested plasmid - (reply: 2)
-
plasmid in 1M tris ..URGENT - (reply: 1)
-
pEBB or pEBG vector maps - (reply: 2)
-
do plasmids amplify more efficiently than genomic DNA? - (reply: 3)
-
What amount of insert:vector should I use if I want a 3:1 molar ratio? - (reply: 2)
-
How to calculate insert:vector ratio? - (reply: 2)
-
plasmid amplification - exons and primers (reply: 1)
-
plasmid sequencing - (reply: 4)
-
structure difference between plasmid & chromosomal DNA - (reply: 1)
-
plasmid DNA dilution for absolute quantitation - (reply: 2)
-
Peg not precipitating out all the phage - (reply: 2)
-
Transformation using linearized plasmid? - (reply: 6)
-
Control Vector - (reply: 4)
-
My plasmid purity changes with different dilutions - (reply: 1)
-
Vector searching - (reply: 1)
-
Plasmid PCR - (reply: 5)
-
lamda DNA digested with HindIII - (reply: 1)
-
Plasmid map for ptrc 99c - (reply: 1)
-
Protocol for RNase treatment of plasmid for RNA cleanup - (reply: 4)
-
No success with PCR cloning of a gene into expression vector - (reply: 5)
-
miRNA overexpression - pol II - type vectors... (reply: 1)
-
low plasmid dna from ET host - Low plasmid Dna concentration in Ecoli ET 12567 (reply: 3)
-
Expression vector - (reply: 1)
-
Plasmid Extraction from a big volume of pellet - Molecular biology (reply: 2)
-
keep getting incomplete digest for plasmid - (reply: 3)
-
Transformation of a 21kb vector - (reply: 11)
-
Plasmid DNA purification - (reply: 3)
-
about antibiotic in plasmid transformation - which is best? (reply: 6)
-
trouble with methlase sensitve restriction digests on plasmids isolated from DAM - (reply: 2)
-
Co-transfection for stable cell lines - mammalian - Need to know the ratio of the plasmids that need to be used (reply: 2)
-
plasmid contamination - (reply: 2)
-
Is re-sequencing needed for insert ligated into a second vector? - (reply: 1)
-
Linear or unlinear plasmid before transfection for expression? - (reply: 2)
-
Homology of plasmid sequence - Is Origin of relication sequence lifted from the bacterial genome? (reply: 2)
-
direct plasmid sequencing problems - direct plasmid sequencing problem (reply: 4)
-
how to co-transfect different plasmids? - (reply: 3)
-
How to get a good quality of plasmid in maxi prep. - (reply: 7)
-
How to extract a 20kb vector after double digestion - (reply: 4)
-
in vitro translation vector - (reply: 3)
-
To make good plasmid for expression transformation - (reply: 1)
-
retroviral vector - [help] pSM2c and LinX (reply: 2)
-
inserts and vector ratio in liagtion Rxns - (reply: 6)
-
Troubles with vector dephosphorylation - troubles with vector dephosphorylation (reply: 2)
-
transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
-
Isolation of episomal plasmid DNA from mammalian cells - (reply: 1)
-
plasmid contaminated with another dna - (reply: 1)
-
plasmid sterilization - (reply: 1)
-
Dual Vector backbones - cloning problems? (reply: 1)
-
can you introduce mutation directly into the cells? - other than just transfecting it with a vector... (reply: 1)
-
Lentiviral vector production timeline - (reply: 1)
-
How to spot plasmid on paper for shipping? - (reply: 4)
-
Do transfected vectors bind histones and/or methylate? - (reply: 10)
-
Plasmid DNA purification - Lithium chlorid (reply: 1)
-
How to elute plasmid spoted on paper - (reply: 3)
-
stability of TOPO vector at RT - (reply: 3)
-
About the procedure of plasmid linearization - How many positions and the proper position? (reply: 2)
-
ligating adaptor in 26kb plasmid - (reply: 1)
-
No plasmid in HB101 - whereas DH5alpha does (reply: 2)
-
Plasmid purification - (reply: 5)
-
does anyone have the pLSA-beta-geo vector map? - (reply: 6)
-
plasmid rescue method - (reply: 3)
-
pCMV-CD19 plasmid map - (reply: 2)
-
Transduction using lentiviral vector - (reply: 3)
-
Do you have any problem with QIAGEN Plasmid Maxi kit - (reply: 5)
-
about siRNA and shRNA - RNAi Vector (reply: 2)
-
pMT2 vector sequence - (reply: 1)
-
Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
-
RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
-
good vectors for neuronal expression - (reply: 13)
-
Where to find list of plasmid copy numbers? - I need to know approximate copy number before I choose vector (reply: 5)
-
how to recognize destination vector in vector NTI - (reply: 1)
-
gel purification of the vector... - is it good enough for ligation? (reply: 7)
-
Plasmid Concentration Calculation - (reply: 9)
-
How to accurately quantify plasmid DNA in solution within impurity of RNA? - (reply: 3)
-
not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
-
purification of digested plasmids - (reply: 4)
-
Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
-
my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
-
Another Problematic Ligation - 110bp ligation into 2.5kb vector (reply: 7)
-
pBABE plasmid - (reply: 3)
-
Need Help ASAP with maxiprep - Diluted plasmid with buffer ER instead of TE (qiagen kit) (reply: 3)
-
isolation of DNA from a plasmid for site-directed mutagenesis? - do researchers actually do this? (reply: 6)
-
is it my recombinant plasmid or contamination? - only two colonies on the plate.... (reply: 5)
-
transformation of a big plasmid in E. coli - something wrong is happening in my transformation (reply: 6)
-
plasmid map software? - (reply: 7)
-
cloning vector - cloning vector (reply: 1)
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which vector will be best for cloning and then expression - vector (reply: 1)
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pBR322 relaxed plasmid....what type of media? - (reply: 3)
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Anyone has the experience to order plasmid from "Addgene"? - (reply: 2)
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Plasmid appears as two bands after double digestion - (reply: 2)
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How to insert one single nucleotide into a plasmid? - (reply: 6)
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Side directed mutagenesis with 17kb plasmid - (reply: 19)
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chloroform v. phenol/chloroform/isoamylalcohol extraction of plasmid DNA - advantages/ disadvantages of each? (reply: 4)
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Differenciation between plasmid, super-coiled DNA and linear DNA - What is the difference between plasmid and other DNA on the gel? (reply: 1)
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RNase treatment of plasmid - (reply: 7)
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Mammalian expression vector - (reply: 3)
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Plant expression vector - (reply: 4)
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ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
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Do TetO seqeunces make plasmid difficult to grow? - (reply: 2)
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Transfering a DNA construct from one vector to another - in frame/not in frame? (reply: 2)
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is the polylinker in pUC18 plasmid a artificially inserted fragment? - want to know whether the polylinker site is a inserted or naturally present in t (reply: 1)
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Phage precipitation techniques? - (reply: 1)
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Miniprep plasmid shows three bands - (reply: 1)
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Expression problem in pQE30 vector system - (reply: 2)
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clarification - plasmid and gel - (reply: 9)
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Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
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vector with tag recommendation - (reply: 10)
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Vector databases and resources - (reply: 6)
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pCIS expression vector - (reply: 2)
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Baculovirus Expression - Sonicate infected cells? (reply: 2)
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Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
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vector sequence - (reply: 3)
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non-expression vector - (reply: 6)
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Vector insertion for beginners - (reply: 4)
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Ligation help needed! - vector religation? (reply: 3)
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plasmids sequences - plasmids sequences (reply: 1)
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No insert for cloning PCR product into expression vector - (reply: 5)
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plasmid incompatibility in mammalian cells? - (reply: 1)
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Maxiprep of plasmid DNA - (reply: 1)
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vectors (PJG4-4, PJG4-6) - maps of PJG4-4, and PJG4-6 vectors (reply: 2)
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pCG plasmid - (reply: 2)
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Ligation is killing me - 1kb insert, 6 kb vector (reply: 11)
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failure of insertion of fragment into large vector - (reply: 1)
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pcDNA3.1 & pBIG2i vectors appear "bigger" on the gel! :( - (reply: 1)
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antibiotics for 3 vectors and sequencing - (reply: 2)
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RT-PCR on plasmid - (reply: 1)
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How to make a T/A vector - (reply: 2)
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which tag and vector is the best for expressing protein in bacteria? - (reply: 4)
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Two same oris (origins of replications) in one plasmid - (reply: 1)
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Best Vector for shRNA production - Help (reply: 5)
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how to isolate plasmid insert? - (reply: 1)
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Retroviral Vector won't digest ideas! - (reply: 1)
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Kozak Sequences and Mammalian Expression Vectors - (reply: 2)
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what would happen if two SV promoter show in one vector - (reply: 1)
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HIS-TAG LIGATION - Ligation of His-tag into the pIMS vector (reply: 2)
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Vector self ligation? - (reply: 1)
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GW1 plasmid sequence - someone know this plasmid???? (reply: 1)
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nonspecific cellular responses - vector system vs synthetic siRNA - (reply: 1)
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yeast plasmid as template - (reply: 1)
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vector map - (reply: 4)
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cloning systems - pGEM-T easy vector system versus TOPO TA cloning? (reply: 4)
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ligating small oligos - how to ligate small oligos into vector (reply: 2)
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vector basics for transgenic mice - (reply: 2)
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Mystery band after vector digestion - (reply: 3)
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putting BAC plasmid back into E. coli - (reply: 7)
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destabilized EGFP Vector urgently needed - (reply: 4)
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Long PCR for amplification from plasmid - (reply: 5)
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sequencing with large plasmids - (reply: 4)
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Why freshly prepared alkaline solution II for plasmid extraction? - (reply: 10)
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How to make a point mutation in a wild type plasmid. - (reply: 2)
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Restriction analysis of a vector - (reply: 17)
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S. aureus plasmids - S. aureus plasmids (reply: 1)
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Crude Plasmid Digests - Help! (reply: 8)
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Purify genomic from plasmid DNA - (reply: 5)
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problems with digesting insert from vector - (reply: 4)
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three way ligation (two fragment and a vector) - (reply: 1)
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how to amplify fragment from the plasmid - (reply: 3)
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Do you have to linearize your vector before transfection? - (reply: 4)
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How to remove small amount of plasmid from filter paper - (reply: 6)
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stable transfection of two plasmids? - (reply: 4)
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a problem of pCMV-Fluc plasmid transfection. - (reply: 1)
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PCR an insert in a vector with wrong product size - (reply: 1)
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A few questions about insertion into a plasmid - (reply: 7)
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phosphorylated oligos and vector - (reply: 2)
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how to amplify a 350bp in length from a vector - (reply: 2)
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transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
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Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest (reply: 2)
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vector with H1 promoter be transfered to mouse cells? - (reply: 3)
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Restriction enzyme SBFI failed to cut plasmid - (reply: 3)
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Calculate insert/vector ratio for ligation - (reply: 8)
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How can I change a promoter of a plasmid? - (reply: 1)
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Concentrating plasmid DNA - (reply: 3)
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Cloning issue - vector insert ratio - (reply: 3)
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promoter selection in plasmid based shRNA - (reply: 2)
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help:failure of extraction of plasmid DNA - (reply: 2)
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Designing siRNA expression vectors - (reply: 2)
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Expression problems with pIRES vector from Clontech - EGFP at MCSB site cannot be expressed! (reply: 1)
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About RNAi based on sh-RNA plasmid - (reply: 2)
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SV40-less Luciferase promoter plasmid? - (reply: 2)
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How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
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Promoter insertion in pUAST vector - Promoter insertion in pUAST vector (reply: 1)
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TA cloning Vector - ...how to prepare it myself (reply: 1)
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cannot isolate plasmid from mutants - please HELP! (reply: 1)
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i need help : lambda phage excision problem !! - labmbda phage excision problem (reply: 4)
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Can't get rid of RNA in my phage extraction. - (reply: 4)
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Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
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Storing low conc plasmid DNA - (reply: 3)
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problems cloning into plenti6/V5 lentiviral vector - (reply: 2)
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Who knows and uses the Plasmid pGag-PolGpt? - Poor plasmid yield with Maxi-Prep.... (reply: 1)
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TOPO TA Vector Cloning problem - no colonies - (reply: 5)
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Why my insert and vector dissappear after mini prep? - (reply: 4)
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Who knows the plasmid M13? - looking for the plasmid map of the m13 plasmid (reply: 1)
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DNA vaccine vectors - (reply: 1)
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By using a plasmid (interested insert) as a templete for PCR - PCR (reply: 2)
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what's wrong? Plasmid digestion produce only smear - (reply: 6)
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How to amplify the insert from a plasmid? - (reply: 2)
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TA Vector to pQE Vector chang problem - Can't get rid of originally TA vector.. (reply: 1)
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No Plasmid digestion - Enzymes don't cut my miniprep Plasmid-DNA (reply: 2)
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Sequencing plasmid gets overlapping peaks - troubleshoot (reply: 5)
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Plasmid digestion - Problem with digestion (reply: 3)
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plasmid transfection and virus infection simutaneously - (reply: 2)
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SDM large plasmid - (reply: 1)
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Problem amplifying 3 kp fragment from a plasmid - (reply: 4)
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How would one go about moving a gene from plasmid to plasmid - cloning (reply: 2)
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Large scale purification of large size plasmid - (reply: 1)
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Transfect/Infect hematopoietic cells - What vector systems to use? (reply: 1)
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Plasmid miniprep problem - (reply: 4)
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Help: anyone who has co-transfected TWO different plasmids - - how much of each DNA to use? (reply: 5)
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Plasmid template preparation for in vitro transcription - (reply: 9)
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Plasmid - Choosing a plasmid (reply: 2)
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non-viral vectors - (reply: 1)
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Plasmid changes after transformation - (reply: 2)
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dephosphate the digested vector - (reply: 4)
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Mysterious gain in vector size after cloning! - (reply: 1)
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plasmid DNA isolation - drying dna samples after isop or 70% EtOH wash (reply: 4)
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Ligation of two oligos into a vector - (reply: 4)
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digestion problem - incomplete digestion of transformed plasmid (reply: 1)
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Growing large plasmid (20kb) in e.coli - Low plasmid yield from large construct (reply: 7)
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plasmid DNA isolation - problem in plasmid DNA isolation (reply: 3)
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Expression Vector Construct - sequence entire insert? (reply: 3)
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Identification of the cut and uncut plasmid on gel - (reply: 4)
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Dual-promoter plasmids? - Trying to transcribe sense and antisense strands.. (reply: 1)
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Inducable mammalian expression vector - (reply: 3)
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Is it ok to store a digested vector for ligation? - (reply: 4)
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should we dilute the DNA (vector and insert), before ligation? - ligation problem (reply: 5)
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How does plasmid DNA miniprep kit get rid of chromosome DNA? - (reply: 4)
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Clontech pEGFP-N1 and -C1 vectors - (reply: 4)
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Plasmid wash in E. coli? - How to promote the lost of a plasmid in coli (reply: 2)
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any effect if the plasmid got 3 gen resistance? - (reply: 1)
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no protein expression after induction using pET vector - (reply: 4)
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Vector Map Software - Know of any free or cheap? (reply: 8)
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Protease in expression vector - (reply: 3)
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Trouble cloning a 8.5 kb fragment into a pTRE vector - (reply: 2)
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Ligation of 2 fragments and one vector - (reply: 7)
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Rolling circle replicating plasmid and transformation - Gene cloning (reply: 2)
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Maximum insert size in a pGEM Teasy vector - (reply: 2)
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Construct DNA plasmid for QPCR - (reply: 4)
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Plasmid preparation problem using alkaline-lysis - (reply: 5)
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Putting 2 plasmids in 1 e. coli cell - (reply: 4)
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antibiotics concentration for ligated plasmid - (reply: 3)
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circular vector propagation - How? (reply: 1)
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No colonies with site-directed mutagenic plasmids - (reply: 2)
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pSUP5011 - what is the molecular size of the plasmid pSUP5011 (reply: 1)
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plasmid extraction and antibiotics matters... - (reply: 4)
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PCR on plasmids? - (reply: 2)
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Plasmid methylation problem - Plasmid methylation (reply: 3)
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Linearized or unlinearized plasmid for stable transfection? - (reply: 18)
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Self ligation of vector - (reply: 2)
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TA cloning - pGEMT easy vector system-not a single colony (reply: 6)
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wash off plasmid in the tube for reuse - question (reply: 1)
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pSVT7 vector sequence or map - (reply: 3)
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real-time pcr plasmid standards - (reply: 4)
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Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
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PCR of plasmid miniprep from Agrobacterium - Can't get PCR product out of plasmid miniprep (reply: 1)
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Whatman Filter Paper - Elution of plasmid DNA (reply: 1)
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How to construct T vector - (reply: 8)
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transfecting siRNA and a plasmid - (reply: 4)
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plasmids turnedinto smears after NcoI digestion - restriction enzyme problem (reply: 1)
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Plasmid sequencing - (reply: 2)
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plasmid sequence - (reply: 3)
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Why ligation reactions are done at 4C - Ligation of insert into vector (reply: 2)
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poor ligation of plasmid/oligo construct - (reply: 8)
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Stable Transfections - do you NEED to linearise the plasmid? (reply: 1)
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Taking OD of plasmid dsDNA in ddH20 - plasmid prep (reply: 3)
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plasmid stock - (reply: 6)
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How to dissolve over-dried DNA? - plasmid prep (reply: 6)
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Plasmids in eluting buffer - (reply: 1)
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pGEM-T easy vector for expression - (reply: 6)
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Storing of miniprep or maxiprep plasmids - (reply: 7)
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PCR an insert in a vector with wrong product size - I can not amplify my fragment with a normal PCR (reply: 8)
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vector auto ligation problem - (reply: 1)
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mammalian expression vector - (reply: 1)
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Human infection from Viral Vectors? - (reply: 4)
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why linearize plasmids make transfection stable? - (reply: 6)
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ligation problem - self ligation of vector - (reply: 5)
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Blunting both vector and insert - klenow and CIPping (reply: 2)
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problems purifying plasmid DNA after restriction digestion - (reply: 3)
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Agarose Gel Electrophoresis of plasmid DNA - Agarose Gel Electrophortesis of plasmid DNA (reply: 1)
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virus vector for transfection - (reply: 8)
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making more plasmid - (reply: 2)
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Vector with T5-promotor in Bl21/DE3-system - Has anyone had experience of the T5-promotor being leaky? (reply: 1)
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Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a (reply: 1)
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How big DNA fragment can I clone to pUC19 plasmid? - (reply: 2)
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protein expression - pkk223-3 vector (reply: 3)
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Lentiviral vector for infecting primary cells? - Any suggestion for a suitable vector? (reply: 1)
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expression vector pHM6's map - (reply: 3)
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de-phosphorylating a vector - (reply: 7)
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Unstable plasmid ?? - Deletions only after ligation ! (reply: 2)
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The amount of plasmid for ligation reaction - Help (reply: 2)
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transfection of single copy of plasmid per cell - (reply: 3)
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AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
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Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
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Plasmid DNA cleaning - (reply: 2)
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Sequencing of Plasmids - (reply: 2)
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Which vector for cloning big PCR fragments? - (reply: 3)
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About identify the inserted gene? - a using PCRII- topo vector question (reply: 4)
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Dephosphorylation of vector before ligation - Is it required? (reply: 38)
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Plasmid drawing tools - User friendly Plasmid drawing tools (reply: 1)
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Plasmid DNA contamination - the source of Chrosomal DNA contamination (reply: 2)
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Plasmid DNA Purification - plasmid was contaminated by Genomic DNA (reply: 1)
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Cloning into shRNA vectors - (reply: 4)
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Vector Vs Host - PGL2 Vs DH5alpha (reply: 1)
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How to prepare enough plasmid for transfection? - (reply: 3)
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Cloning into GFP vector - (reply: 2)
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Plasmid DNA storage - (reply: 4)
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purify linearized plasmid for transfection - (reply: 1)
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question about determinating the size of plasmids - (reply: 2)
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question about plasmid linearization - (reply: 2)
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about vectors and transfection of RNAi - (reply: 1)
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Low copy number of plasmid - (reply: 3)
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Can shRNA be transfected without vector...?? - Planning new experiments (reply: 3)
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plasmid restriction map- free software - (reply: 3)
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Help: baculovirus expression system? Anyone's used it? - Has anyone used this system (reply: 1)
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Bad OD ratios from Plasmid Minipreps - (reply: 2)
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cloning problem - cloning problem in GFP vector (reply: 4)
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Problem cloning PCR product into double-cut vector - (reply: 3)
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RNAi vector for stable expression - (reply: 4)
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Plasmid gene mutation - (reply: 1)
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sucide plasmid directed mutagenesis - sucide plasmid directed mutagenesis in pseudomonas (reply: 1)
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Need help in plasmid sequencing - (reply: 4)
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Subcloning into large plasmid - (reply: 3)
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plasmid digest - restriction enzyme won´t cut (reply: 1)
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ligation problems and searh for map of pGW1 vector - (reply: 1)
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Freezer culture fails to retain plasmid - (reply: 3)
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doing phage titrering - (reply: 1)
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help for large-scale plasmid purification - (reply: 3)
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Suspicion of Phage infection - Urgent, pls help (reply: 2)
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T- vector clone - (reply: 3)
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Does my bacteria have a plasmid? - (reply: 5)
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Subcloning into lentiviral vectors - Very little to no plasmid after miniprep (reply: 7)
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DNA plasmid extraction trouble - (reply: 1)
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Software for plasmid drawing - (reply: 1)
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Plasmid DNA purification - DNA purification (reply: 7)
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cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
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removing plasmids from yeasts - (reply: 1)
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How to ligate ds-oligos into a vector? - (reply: 48)
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transformation - Possible to get two diff. plasmids? (reply: 1)
-
wierd plasmids: maxi-prep never work - (reply: 13)
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plasmid draw - Need software for Vector cloning design (reply: 4)
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Problem with plasmid linearization - (reply: 2)
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Plasmid extraction from E.coli culture - (reply: 7)
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Promoterless Retroviral vector? - (reply: 1)
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plasmid went missing - what happen?? (reply: 5)
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vector pFPV25.1 - need help!!! (reply: 2)
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Quickchange site directed mutagenesis - Success with a 13 kb plasmid? (reply: 2)
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stable transfection with siRNA expression vector - (reply: 6)
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Shipping of plasmids and X-rays - (reply: 1)
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the vector for stable transfection - (reply: 3)
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plasmid sequence - IncQ (reply: 1)
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vector map - (reply: 2)
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vector sequence - pBABE vector sequences (reply: 7)
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the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
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how to add flag to a vector - (reply: 7)
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blunt-cloning - all are vector self-ligation products? (reply: 33)
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42bp fragment ligation to 4776bp vector - (reply: 3)
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Cloning of high-GC% gene into vector - (reply: 3)
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Big Size of Plasmid Transformation - Urgent! (reply: 1)
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Site directed mutagenesis - Of 20 kb plasmid (reply: 1)
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plasmid for stable transfection - (reply: 1)
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Plasmid problem - (reply: 2)
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Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
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Plasmid DNA extraction - Extracted plasmid cannot be seen in agarose gel (reply: 9)
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RNA in plasmid preparation interfere in ligation - RNA contamination and ligation (reply: 3)
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phage PCR - (reply: 1)
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How do I extract a HUGE plasmid? - (reply: 1)
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Expression vector/host for plant genomic sequence - (reply: 3)
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plasmid mini-prep from E.coli - smeared bands (reply: 2)
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plasmid can't be digested by enzyme - (reply: 13)
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Plasmid Extraction Kits Manufactured by? - Plasmid Extraction Kits Manufacture (reply: 1)
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how to identify whether the PUC18 plasmid is good or not - about the PUC18 (reply: 1)
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Transformation technique's and [abx] affect on plasmid yield - plasmid production and isolation (reply: 1)
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Vector Suggestion needed - (reply: 1)
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Met trouble in ligation! - insert:sacII,XhoI;vector:sacII,SalI (reply: 1)
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How to transform the cells witht wo different plasmids - (reply: 3)
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recover a plasmid about 50kb with high yield? - (reply: 1)
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cDNA library (in Lambda ZAP vector) screening - picking up plaques on nitro cellulose membrane as replica (reply: 2)
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Vectors - (reply: 1)
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ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
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M13 phage amplification,who canhelp? - (reply: 2)
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anybody can provide me with aplasmid vector which can be visually scre - (reply: 3)
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Where is a good vector databaseon the internet? - (reply: 1)
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Prk5 plasmid - mammalian experssion vector (reply: 1)
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molecular biology - cloning into pPic9 vector (reply: 1)
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BAC vector problems - (reply: 1)
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Removal of Rna from plasmid prep - (reply: 1)
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Plasmid prep doesn't work! - Help needed with HB101 strain plasmid prep (reply: 3)
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question about plasmid digested by one-cut-site RE - (reply: 3)
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Co-transfection Question - Will co-transfected vectors enter the same cell? (reply: 2)
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ligate 3 DNA fragments with sticky ends into Vector - (reply: 1)
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Yeast plasmid extraction - (reply: 5)
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plasmid isolation - (reply: 6)
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Plasmid isolation again - Isolation from multiple colony? (reply: 1)
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Have anyone ever used this plasmid? - (reply: 2)
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Re: How to transform thecells with two different plasmids with d... - (reply: 1)
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trouble in plasmid pcr - i cannot obtain designed band from a definied plasmid (reply: 4)
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how to isolate huge plasmid DNAfrom Pseudomonas putida - (reply: 2)
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Plasmids purification - how to remove DNA contamination (reply: 3)
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How to clone large DNA fragment into small vector! - (reply: 1)