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Vector Related Discussions
  1. Plasmid pCP20 problem - (reply: 1)
  2. Creating empty vector - (reply: 5)
  3. cloning vector and restriction mapping - (reply: 2)
  4. software to replace Vector NTI and a simple cloning problem - (reply: 3)
  5. Stable transfection problem - Problem with pBM vector and Phoenix packaging cells (reply: 3)
  6. plasmid - (reply: 2)
  7. no expression in pET 21 vector - (reply: 2)
  8. DNA purification - Touble with isolating PCGP plasmid (reply: 3)
  9. Good vector for genomic library - (reply: 2)
  10. NO expression in pKK223-3 vector in DH10B Why ? - in DH10B NO expression in pKK223-3 vector in DH10B Why ? (reply: 2)
  11. [Help] New vector construct problem - (reply: 4)
  12. contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
  13. why use beta galactocidase as a positive control in co-transfection - why not an antibiotic resistant plasmid (reply: 13)
  14. Does plasmid DNA mutate over time? - (reply: 2)
  15. suitable vectors for 3' UTR Luciferase? - (reply: 2)
  16. miRNA expression vector - (reply: 3)
  17. Plasmid DNA storage - (reply: 1)
  18. Vector NTI no longer free - (reply: 7)
  19. co-transfgection - antibiotic plasmid instead of B galactocidase (reply: 2)
  20. directional cloning problem - two-digested vector and insert - ligate - no colonies?? (reply: 2)
  21. plasmid isolation problem HELP! - (reply: 2)
  22. Cloning into pGBKT7 vector - (reply: 4)
  23. pRARE2 vector sequence - (reply: 4)
  24. plasmid preparation - (reply: 10)
  25. Plasmid DNA concentration - (reply: 2)
  26. Plasmid isolation from bioluminescence bacteria - Alkaline lysis (reply: 5)
  27. pGL-3 vector transformation - (reply: 5)
  28. Phosphatasing to prevent vector reclosures - (reply: 1)
  29. A problem with plasmid DNA extraction - (reply: 6)
  30. searching for a piece of plasmid information - (reply: 1)
  31. IPTG induction in phage display techniques - (reply: 2)
  32. The vector became smaller - (reply: 4)
  33. eukaryotic inducible vector with GFP-tag fusion? - (reply: 3)
  34. DsRed stable transfections? - problems with Stoplight vector in Flip-in cells (reply: 4)
  35. phrGFP-1 - searching for vector sequence - (reply: 6)
  36. Working with TOPO II cloning vector - (reply: 2)
  37. Novagen pet 32 Ek/LIC vector - (reply: 2)
  38. Easiest cloning strategy for deleting part of plasmid using adapters? - (reply: 1)
  39. Plasmid Isolation - (reply: 1)
  40. Problem with GST tagged baculovirus protein - (reply: 2)
  41. non dephosphorylated vector gave white colonies - (reply: 2)
  42. plasmid isolation and gel - (reply: 1)
  43. How to transform with topo vector - (reply: 6)
  44. pET-16b vector in DH5alpha E. coli - (reply: 7)
  45. Using pGEMT Vectors - (reply: 4)
  46. Purification of RF DNA from phage prep - Need to get rid of ssDNA contamination (reply: 2)
  47. Cloning of plasmid - (reply: 3)
  48. Expression vector and Stop codon consideration - (reply: 2)
  49. No DNA in 1 of 6 plasmid preps (Qiagen - Midi) - (reply: 2)
  50. transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
  51. Two CMVmin in same plasmid? - (reply: 2)
  52. Who has a good protocol to cut a plasmid? - (reply: 1)
  53. Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate? (reply: 8)
  54. Is there any way to remove chromosomal DNA from plasmid DNA? - (reply: 6)
  55. phage titer problem - (reply: 1)
  56. How to construct miRNA expression vector - (reply: 1)
  57. Large MSCV Retroviral Vector Cloning --- Help Needed! - (reply: 3)
  58. how to get rid of a plasmid? - (reply: 7)
  59. Erroneous PCR product due to plasmid DNA contamination - (reply: 2)
  60. vector seq & Multiple cloning site design. - (reply: 2)
  61. Single Vector multiple promoters? - (reply: 8)
  62. Medical significance of plasmids - (reply: 3)
  63. Problem of Plasmid Preparation - (reply: 7)
  64. Attaching DNA bases - Creating primers and plasmids? (reply: 3)
  65. How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
  66. how many plasmids can a competent bacteria take - (reply: 9)
  67. Insert/vector ratio for ligation - (reply: 3)
  68. Baculovirus expression problem - Mixture of Bacmid DNA (reply: 2)
  69. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
  70. how to clone my insert DNA into vector? - (reply: 11)
  71. methylation of plasmids - (reply: 1)
  72. plasmid digestion gel - (reply: 4)
  73. Rearrangement in plasmid? - (reply: 2)
  74. restriction digestion of plasmid - (reply: 8)
  75. methylated plasmids in culture - (reply: 1)
  76. How to induce expression in mammalian cells with the pcDNA/V5/GW vector ? - (reply: 3)
  77. Plasmid transfection - should amount be adjusted according to insert size? - (reply: 4)
  78. plasmid miniprep - (reply: 1)
  79. cohesive-end ligation - cloning a fusion protein to a vector (reply: 1)
  80. Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
  81. TAT-GFP expression from pRSET vector - (reply: 1)
  82. plasmid transfection - (reply: 1)
  83. Expression Vector sequencing problem - (reply: 6)
  84. Expression Vector sequencing problem - (reply: 1)
  85. Trouble inserting PCR derived insert into vector - Trouble inserting PCR derived insert into vector (reply: 2)
  86. Cloning with methylated vector - (reply: 2)
  87. Plasmid Prep Problem : gDNA - bacterial genomic DNA contamination in a plasmid minipreparation (reply: 3)
  88. Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
  89. Using IRES in microRNA expression vector - (reply: 4)
  90. Any PBI121 Vector Information ? - Map, Sequence etc. (reply: 2)
  91. how to check the insertion of a gene fragment into plasmid - (reply: 2)
  92. Problems during plasmid prep in cloning - (reply: 7)
  93. Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
  94. Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
  95. Large vector self-ligation in presence of insert (1:1)? - (reply: 3)
  96. How to find plasmid containing 35S and Nos ? - (reply: 3)
  97. Need to sequence gel purified vectors exposed to UV? - (reply: 2)
  98. How to recover old baculovirus stocks - (reply: 1)
  99. Improving transformation efficiency - large plasmids (reply: 6)
  100. Plasmid lost during culture - (reply: 19)
  101. TOP10 competent cell with another vector - (reply: 1)
  102. Help restriction analysis of plasmid - (reply: 11)
  103. High Yielding Plasmid Purification (suggestions) - (reply: 1)
  104. basic questions regarding expression vector work - (reply: 2)
  105. Can I store plasmid in 4C refrigerator? - (reply: 11)
  106. BL21 Plasmid extraction? - (reply: 4)
  107. commercial TA cloning vector with Luciferase - cloning promoter sequences (reply: 2)
  108. Help...cell transfected with empty vector(control) don't retain fluorescence - PIRES2 (reply: 3)
  109. T1 phage infection - can it be due to poor lab practice? (reply: 4)
  110. How to induce self-ligation of a vector? Self-Circularization? - (reply: 12)
  111. MIDI's low recovery or vector issues? - (reply: 2)
  112. plasmid copy number - experiments on how to compare the copy numbers of different copy-numbe (reply: 3)
  113. Problems subcloning with the pL452 recombineering plasmid - (reply: 2)
  114. No Ni-NTA binding--internal start codon of NdeI in pET vector? - (reply: 1)
  115. difference between subculture in incubator and shaker - on preparation of cultures for plasmid dna extraction, is there a diff (reply: 3)
  116. which part of gene is needed for normal expression in vector - (reply: 2)
  117. Screening Tranformation Colonies: Funny Gel Pic and no plasmid?!?! - (reply: 10)
  118. HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
  119. How to get an individual plasmid out of a plasmid mix in E.coli - (reply: 6)
  120. upscaling protein production - is type of vector and host strain crucial factors? (reply: 1)
  121. How to confirm whether the cell line was successfully transfected with two plasm - Co-transfection of two plasmids to 293 cells (reply: 6)
  122. High concentration of antibiotics in amplification of plasmids - concerns about mutation of inserts in plasmid (reply: 1)
  123. Plasmid DNA preparation - two DNA bands in gel (reply: 5)
  124. Databases of commercially available plasmids - (reply: 2)
  125. cloning into plasmid (IMPACT kit from NEB) - (reply: 3)
  126. help with luciefarse reporter assay,:( - p53 reporter vector versus expression plasmid (reply: 1)
  127. ta cloning vectors - non commercial ta cloning vectors (reply: 1)
  128. how to extract plasmids leaving bacteria cells intact - (reply: 7)
  129. Plasmid Shelf Life? - (reply: 1)
  130. No protein expression in pKK223-3 vector in JM105 - (reply: 3)
  131. Concepts about the Vector - (reply: 2)
  132. How to test if a cDNA clone from a plasmid is infectious? - (reply: 4)
  133. RNAi vector construction - Has anybody done it? (reply: 2)
  134. plasmid prep - help (reply: 8)
  135. In vitro Transcription - Do I need to linearize my plasmid ? (reply: 1)
  136. multiple plasmids in cells - (reply: 1)
  137. QUestion on subcloning a 80bp product from TOPO vector - (reply: 5)
  138. TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
  139. Sequencing result is vector self-ligation while bacterial PCR identification was - (reply: 5)
  140. plasmid transfection in hepatocytes - (reply: 1)
  141. Vector replication - (reply: 4)
  142. RE cloning a 2.3kb fragment into a 4.7kb vector - (reply: 4)
  143. How can I create a T vector for PCR product cloning - (reply: 2)
  144. Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
  145. how to insert a thrombin cleavage site - ....into my vector (reply: 3)
  146. inducible retro/lentiviral vector - (reply: 5)
  147. luciferase vector construction - (reply: 3)
  148. Why there is a artifical intron in some plasmids? - (reply: 4)
  149. Retroviral vector cloning help - (reply: 6)
  150. cloning with pGEM T-easy vector - question about the vector. (reply: 2)
  151. Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
  152. Fate of plasmid - How to detect plasmid DNA on host chromosome (reply: 7)
  153. How to search plasmid sequence for restriction sites and resistance genes? - (reply: 4)
  154. linearized vector purification from TAE agarose gel - (reply: 5)
  155. help with His-tagged vector construct - (reply: 2)
  156. Plasmid with two different origins? - (reply: 1)
  157. EcoR1-Pst1 digest for vector and insert - (reply: 3)
  158. Vector digestion - (reply: 6)
  159. sequencing vector DNA - (reply: 4)
  160. empty vector control - (reply: 4)
  161. Vector concentration following maxiprep - (reply: 4)
  162. How about vector sequence in cloning? - (reply: 9)
  163. How to get rid the restriction sites in a plasmid ? - (reply: 5)
  164. Expression vector for Pseudomonas - expression of a 10Kb gene in Pseudomonas aeruginosa (reply: 2)
  165. gene from 4-TOPO to expression vector - (reply: 1)
  166. PCR product of unlinearized plasmid - (reply: 1)
  167. MoBio Plasmid Purification and Gel Extraction Kits - Problems with MoBio Kits (reply: 3)
  168. Can I store bacteria cells at +4C before plasmid isolation? - (reply: 3)
  169. Multiple recombinant plasmids in transformed E Coli? - (reply: 2)
  170. Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (reply: 1)
  171. Plasmid transformation - (reply: 4)
  172. Mini plasmid Kit extraction - (reply: 4)
  173. why a low copy number? - shRNA vector (reply: 3)
  174. Small fragment sticky-end ligation into EGFP-N1 vector - (reply: 2)
  175. Miniprep for pGEM vectors - please help - Technical help (reply: 7)
  176. Uncomplete plasmid after transformation - (reply: 1)
  177. vector: Klenow + CIP - do they work together?! (reply: 1)
  178. advice re: shRNA vector sequencing - (reply: 4)
  179. problem with restriction digest - I have an insert, but no vector.... (reply: 5)
  180. Help with Maxiprep plasmid low ratio - (reply: 2)
  181. Annealing plasmid DNA - (reply: 3)
  182. cyanobacteria inoculation by phage - inoculation of cyanobacteria by phage (reply: 1)
  183. GFP-Hygromycin vector - (reply: 2)
  184. Plasmid vs Lentivirus as a transfection vector - Opinions? (reply: 4)
  185. Fast way to get plasmid for PCR - (reply: 2)
  186. Transformation of pCMV-sport6 vector to DH5 alpha competent cells - (reply: 1)
  187. how to know the plasmid or phage inside the cell? - (reply: 3)
  188. Help. Trying to sequence my plasmids, but the primers refuse to prime! - (reply: 1)
  189. Plasmid coli glycerol stocks @ -20 - will they still be useable? (reply: 4)
  190. Maxi plasmid preparation - (reply: 3)
  191. Question about maxi plasmid preparation - (reply: 1)
  192. Ligation, plasmid rescue problem! - PLasmid transformation after ligation problem (reply: 7)
  193. DH5 keep kicking out my vector - (reply: 2)
  194. does low plasmid concentration affect transfection rate? - (reply: 4)
  195. Plasmid DNA extraction problem - (reply: 3)
  196. Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
  197. genomic DNA contamination during plasmid preparation - (reply: 1)
  198. Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
  199. plasmid concentration and freezing - (reply: 4)
  200. puc19 vector - (reply: 1)
  201. Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (reply: 2)
  202. pEGFP N1 vector - (reply: 6)
  203. large plasmid extraction? - how? (reply: 5)
  204. no plasmid extracted - low copy number - (reply: 2)
  205. Need help with application of different vectors - (reply: 1)
  206. cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?? (reply: 2)
  207. plasmid subcloning - (reply: 1)
  208. ELISA and phage display - (reply: 7)
  209. basics about expression vectors - (reply: 2)
  210. no plasmid after extraction - (reply: 2)
  211. how can I concentrate my plasmid DNA? - (reply: 3)
  212. need help to solve problems with plasmid isolation - molecular biology (reply: 6)
  213. Suggest a name of a bidirectional expression vector - Anyone has idea of this vector? (reply: 1)
  214. Miniprep plasmid DNA - How clean is it? - (reply: 3)
  215. P/C/I extraction of plasmid after digestion - (reply: 1)
  216. PLasmid extraction: good nanodrop reading but NOTHING on the gel! - (reply: 4)
  217. differential binding of dye to supercoiled and relaxed plasmid DNA - which dye and how? (reply: 5)
  218. smear when cutting plasmids previously checked - (reply: 4)
  219. co-transfection of plasmids - ratio - shRNA plamid with plasmid expres'ng target (reply: 8)
  220. lentiviral vector cloning problem - (reply: 1)
  221. Frameshift in pET vector? - (reply: 7)
  222. compare pEGM T easy vector and TOPO TA cloning - (reply: 7)
  223. miRNA lentiviral vector library - (reply: 1)
  224. High firefly luc activity in EMPTY pGL3 Basic vector - (reply: 2)
  225. pGEM vectors - (reply: 3)
  226. recombinant plasmids lossing - get HELP! (reply: 5)
  227. pT7SC vector - (reply: 1)
  228. Problems cloning a tiny insert into a huge vector - (reply: 1)
  229. Self Circularization Problem with a 6KB vector - self ligation of a 6KB vector (reply: 1)
  230. Blunt end vector........? - (reply: 2)
  231. ligating into vector: sequencing trouble - vector sequences before RE but not after (reply: 1)
  232. How to amplify a phage display library - (reply: 1)
  233. please recommend some common miRNA expression vectors - (reply: 2)
  234. Stability of plasmids in recA1 strains - (reply: 4)
  235. Plasmid problems - low yeild plasmid DNA (reply: 3)
  236. too much background in vector preps - Vector prep (reply: 6)
  237. cloning 708bp PCR prodyct into pET101 vector (TOPO) - (reply: 2)
  238. shotgun cloning: gel shows inserts but only plasmid sequence obtained - (reply: 1)
  239. Plasmid replication in mammalian cell systems - (reply: 3)
  240. +and - in plasmid names - (reply: 2)
  241. PCR of lambda phage - (reply: 1)
  242. Checking Insert using PCR from plasmid DNA? - (reply: 4)
  243. transfect from full plasmid and transfect from PCR product - (reply: 5)
  244. What EDTA does in annealing of DNA and vector? - (reply: 2)
  245. X-ray scanning of DNA plasmids - (reply: 1)
  246. cloning in expression vector - (reply: 5)
  247. Linear plasmid for transfection - where should I cut the plasmid? (reply: 3)
  248. plasmid DNA maxi prep - (reply: 1)
  249. Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
  250. Getting smear problems in plasmid run - (reply: 8)
  251. Elute DNA from Watmann paper - I received a plasmid in the mail and cant get transfectants (reply: 2)
  252. co transfection plasmid and siRNA - message from gmgabriel68 (reply: 1)
  253. identifying genomic DNA contamination during plasmid isolation - (reply: 2)
  254. E.coli promoterless lacZ vector - (reply: 2)
  255. How to chose vector and E.coli strain for protein expression - (reply: 2)
  256. plasmid degradation in tris EDTA - (reply: 10)
  257. E.coli TOP10 comp cells T1 Phage resistant? - (reply: 1)
  258. Question about QuikChange II mutagensis method - Can it merge plasmids? (reply: 3)
  259. Transformation problem with newly ligated plasmids - (reply: 1)
  260. Low transfection efficiency using PEI - I'm trying to transfect BHK-21, with a large size plasmid (13kb), (reply: 9)
  261. qPCR and plasmid positive controls - Why use plasmid positive controls? Are they the best? If so, are there (reply: 1)
  262. pGEX-2T GST fusion vector - (reply: 1)
  263. Recombinant plasmid lost afer transformation - (reply: 3)
  264. Lost in plasmid preperation - (reply: 4)
  265. What is a binary vector? - (reply: 1)
  266. Low yield plasmid extraction - (reply: 6)
  267. Plasmid Cleanup - Advice on how to further clean up plasmid created by alkaline lysis an (reply: 4)
  268. NheI, EcoRI vector double digest - Troubling getting second enzyme to cut (reply: 6)
  269. Gateway cloning with 3 entry vectors - (reply: 4)
  270. vector dephosphorylation and ligation - how does it work? (reply: 1)
  271. Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
  272. Separate cutting plasmid from uncut? - (reply: 4)
  273. plasmid DNA purification - which kit should I buy? (reply: 7)
  274. plasmid prep with protein? contamination - (reply: 3)
  275. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
  276. pEBG vector...Where is it? - Is it possible to purchase this vector? (reply: 3)
  277. how to store plasmid samples? - (reply: 3)
  278. Plasmid extraction from filter paper - (reply: 3)
  279. plasmid prep question - thick white material? (reply: 11)
  280. help finding eukaryote expression vector - (reply: 2)
  281. plasmid - (reply: 4)
  282. No colonies with TOPO vector - (reply: 3)
  283. Need PLen Plasmid map for stable line set up - (reply: 4)
  284. Plasmid Ligation does not work-suggestions? - (reply: 6)
  285. Problem about plasmid constrution - (reply: 2)
  286. pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
  287. Why do you need your insert in the vector in both orientations? - Cloning (reply: 4)
  288. Preference in isolation of plasmid DNA methods? - (reply: 5)
  289. low plasmid yield - (reply: 8)
  290. question on pGEM-T-easy vector system - (reply: 3)
  291. Detection of 10 plasmid copies - detection limit (reply: 5)
  292. concentration of vector in ligation reaction - (reply: 4)
  293. Trouble with viral vector --> too many colonies in control plate - Trouble with viral vector --> too many colonies in control plate (reply: 6)
  294. purposes of uncut plasmid DNA - (reply: 4)
  295. Protein expression from bacteria which has two plasmids? - Incompatibility of plasmids (reply: 5)
  296. Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
  297. how to extract plasmid spotted on paper? - (reply: 7)
  298. Which vector would you choose for transient expression in CHO cells - (reply: 2)
  299. cloning of 1 kb fragment in 14 kb vector - (reply: 3)
  300. adapter liagation into a single cut plasmid? - (reply: 4)
  301. Restriction digested plasmid - (reply: 2)
  302. cloning of a big Plasmid - (reply: 3)
  303. constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
  304. the ratio of pGL3 promoter( control) vector in Dual luciferase assay - Dula luciferase assay (reply: 1)
  305. E.coli Compotent Cell Storage and Plasmid Isolation - (reply: 3)
  306. Blunt Cloning Large Insert/Smaller Vector - (reply: 9)
  307. phage cDNA library screening with PCR - (reply: 3)
  308. miRNA expression vector - miRNA expression vector (reply: 4)
  309. Gel purification of plasmid after each of these steps? - (reply: 1)
  310. Plasmid pSPL3 - Looking for plasmid pSPL3 (reply: 2)
  311. why my plasmid became shorter? - (reply: 5)
  312. plasmid DNA not digested - (reply: 3)
  313. how the multiple cloning site in cloning vector is create? - (reply: 4)
  314. plasmid problem - (reply: 3)
  315. How to mix your plasmid? - (reply: 7)
  316. transformation competent cells problem - fastbac vectors (reply: 2)
  317. dephosphorylated vector - (reply: 1)
  318. one plasmid into each E.coli? - (reply: 13)
  319. plasmid profile - difficulties with marker and size of plasmids.. (reply: 4)
  320. need help in interpreting PAGE - weird banding fr phage PAGE (reply: 1)
  321. Electroporation with a pool of vectors with different inserts - why only one kind of vector maintained in the cells? (reply: 2)
  322. Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
  323. why my plasmid is degraded? - (reply: 4)
  324. Searching for vector sequences - (reply: 2)
  325. Mutation in plasmid - (reply: 2)
  326. peptide expression vector - I need to find a vector for bacterial expression of peptides of varyin (reply: 4)
  327. Is time of ligation insert and vector crucial? - (reply: 4)
  328. plasmid extrction problem! or more! - (reply: 4)
  329. HELP! About plasmid transfection in 96 well plate - (reply: 3)
  330. Retrieve dried vector - (reply: 2)
  331. propagate pTRE-Tight vector problem - (reply: 6)
  332. Is this genomic DNA? - or plasmid? (reply: 11)
  333. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
  334. unable to digest my recombinant plasmid! - (reply: 9)
  335. blunt ending the vector followed by the ligation - (reply: 1)
  336. Vector for expressing proteins to be secreted - (reply: 3)
  337. Vector for producing Fc fusion proteins - (reply: 1)
  338. Can bacteria grow without a plasmid or am i stupid? - or did ampicillin wear out? (reply: 5)
  339. E. coli transformation issues - old plasmids (reply: 7)
  340. Large Scale Extraction of Bacteria Plasmid DNA - (reply: 1)
  341. problem with transformed plasmid's size - (reply: 10)
  342. Inability to grow plasmid in culture - Molecular cloning (reply: 3)
  343. Low or high copy plasmid ? - (reply: 2)
  344. pNUT vector map - (reply: 1)
  345. plasmid DNA Digestion - (reply: 5)
  346. plasmid purification - (reply: 3)
  347. pBinAR vector map - (reply: 1)
  348. MigR1-GFP vector transfection - Did anyone transfect MigR1 using TransFast (reply: 4)
  349. Purification of 13 kb plasmid from E. coli - (reply: 4)
  350. insert:vector ratio for ligation - religation of the vector? (reply: 2)
  351. pET vector problems (HELP) - pEt 21 a (+) (reply: 3)
  352. TE diluted plasmids and transfection - (reply: 2)
  353. HELP! how to retrieve plasmid from glycerol? - (reply: 2)
  354. Please save me ! - pAdeasy adenovirus vector system (reply: 1)
  355. Problem with cloning pGEMT easy vector system - (reply: 4)
  356. pCAMBIA vector map - (reply: 1)
  357. litic phage contamination during panning! - (reply: 3)
  358. Molar Ratio of Inser to Vector - (reply: 3)
  359. What type of PCR to identify a plasmid insert? - (reply: 4)
  360. Protocol for amplification of gene in plasmid directly from transformed e. coli? - (reply: 2)
  361. competent cells transform 12 kb plasmid,urgent - (reply: 8)
  362. Name of double his tag vector - looking for name of company that sells the dual his tagged vector (reply: 3)
  363. Mammalian protein expresion in bacteria - Which plasmid and strain to use (reply: 7)
  364. How can I calculate the copy number of plasmid DNA after mini-prep? - (reply: 5)
  365. reporter vector cotransfection - (reply: 4)
  366. Can a single restricted vector spontaneously ligate back to a circular DNA? - (reply: 3)
  367. Problem: Plasmid DNA extraction from S. aureus - (reply: 6)
  368. integration vector question - do i need to clone a full gene (reply: 1)
  369. Stable transfection using plasmid with no selection gene - (reply: 2)
  370. plasmid cloning in E.coli - (reply: 2)
  371. Restriction of pichia vector pPICZ alpha with Xho1 and Xba1 - (reply: 1)
  372. what is the plasmid nomenclature: eg.what does it mean by pUC, pCR, and other ve - (reply: 13)
  373. Low insert and vector concentration - (reply: 7)
  374. plasmid map drawing - (reply: 2)
  375. trouble cloning into red vector - (reply: 1)
  376. miRNA effect on 3'UTR cloned into a reporter vector - (reply: 2)
  377. pUASp sequence - Need the pUASp vector seq (reply: 1)
  378. Sequencing a 10kb length of dna in a 12kb plasmid - (reply: 3)
  379. help with moving an insert to a new vector - (reply: 4)
  380. Endogenous/Native Plasmid Isolation - (reply: 1)
  381. pGEX vector - (reply: 4)
  382. E. coli plasmid info - (reply: 1)
  383. Plasmid Curing - (reply: 1)
  384. Plasmid Curing - (reply: 7)
  385. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
  386. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
  387. How to add a lethal gene to my plasmid for cloning selection? - (reply: 3)
  388. How to save my plasmid - I run out of my plasmid and my EColi doesn't grow (reply: 3)
  389. Problems w/ yield from lentiviral plasmid prep - (reply: 3)
  390. question:problem during the plasmid isolation - (reply: 7)
  391. Decreasing plasmid size via (digestion-gel extraction-ligation-transformation) - (reply: 8)
  392. phage display library - question (reply: 1)
  393. F1 origin is it mandatory for a plasmid - (reply: 3)
  394. How to improve 260/280 reading for DNA? - 260/280 for purified digested plasmid is very high. (reply: 2)
  395. Elementary Question - How do I send plasmid DNA?? (reply: 11)
  396. Plasmid purification - Purification using Qiagen kit (reply: 2)
  397. Plasmid stability over a 100%? - Why does ampicillin increase cell viability? (reply: 4)
  398. HELP! GFP plasmids - GFP plasmids (reply: 4)
  399. TA-cloning vector contamination - (reply: 3)
  400. Cosmid and plasmid at the same time - (reply: 1)
  401. Vector with two promoters - Molecular Biology (reply: 2)
  402. Transformation-Min plasmid reqd - (reply: 3)
  403. PLASMID DNA yield - (reply: 3)
  404. Can CMV promoter be used as transgene vector - (reply: 2)
  405. plasmid glycerol stock - (reply: 2)
  406. cloning/ligation problem? (shRNA into vector) - cloning, colonies in neg control (reply: 4)
  407. Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
  408. plasmid isolation help urgent.. - (reply: 4)
  409. Very huge vector for transformation - (reply: 4)
  410. pGM-T vector problem - (reply: 3)
  411. pgl3 - empty vector upregulated as much as construct! - (reply: 2)
  412. How much plasmid for transformation? - (reply: 5)
  413. No virus titer and MSCV-mir30 vector problem - HELP (reply: 12)
  414. Plasmid DNA Sequencing with Universal Primers - (reply: 3)
  415. MSCV mir30 retroviral vector problem - (reply: 2)
  416. SubCloning binary vector - (reply: 5)
  417. Phage Display: Not getting antibodies after 3 rounds of panning - (reply: 6)
  418. question about plasmid transfection - (water solution or buffer solution?) (reply: 3)
  419. plasmid molar concetration/copy number - (reply: 2)
  420. how the lentiviral vector transduct into cells? - ask a question (reply: 3)
  421. When double-transfecting, do both plasmids always go together? - (reply: 4)
  422. How many plasmid DNA copies per cell? - (reply: 1)
  423. Need help getting rid of plasmid in E.coli (Rosetta) - (reply: 4)
  424. Retroviral vector - (reply: 3)
  425. MSCV LTRmir30 vector sequencing! - (reply: 2)
  426. plasmid copy number - how one can determine plasmid copy number in E.coli (reply: 2)
  427. Vector keeps on self ligating - help (reply: 3)
  428. Plasmid Extraction - Miniprep incident (reply: 3)
  429. plasmid bands - (reply: 1)
  430. Heating the vector and insert before ligation - new idea for reducing background colonies (reply: 7)
  431. Viral / phage promoter prediction - (reply: 1)
  432. Cloning problems into a big (19 kb) vector - (reply: 10)
  433. De novo plasmid methylation in eukaryotic cells - (reply: 3)
  434. Reconstitution of plasmid - (reply: 1)
  435. Clone 2kb and 5 kb inserts into 6kb vector - Which order and vector:insert ration? (reply: 3)
  436. what is the mechanism of transformation of a plasmid into a bacterial cell? - (reply: 5)
  437. about ligation problem - insert and vector size (reply: 9)
  438. my vector contain ccdB gene in MCS, can i use this as mock control during transf - (reply: 1)
  439. Modification of Vector - (reply: 1)
  440. Making more empty vector - (reply: 7)
  441. M13 phage pfu determination - (reply: 1)
  442. shRNA lenti vectors - (reply: 5)
  443. how to purify plasmid and genomic dna - (reply: 3)
  444. does size matter - how many genes in 1 vector (reply: 10)
  445. Ligation of a 1.8kb insert into 10.8kb vector - (reply: 4)
  446. Religated vector despite a "no insert control" - help! - (reply: 2)
  447. insert to vector ratio - (reply: 3)
  448. Will self-ligated plasmid run faster than the unligated one? - (reply: 9)
  449. DNA degradation after plasmid mini preps - (reply: 6)
  450. Problem transfecting large plasmid into HEK293 cells - (reply: 8)
  451. Stable Clone establishment using Hygromycin or Puromycin vectors - (reply: 3)
  452. Vector with many restriction site - (reply: 6)
  453. Free Plasmid Miniprep Kit - Beta Test (a 42 Dollar value) - (reply: 7)
  454. how to ligate random oligos to vector to construct a library? - (reply: 6)
  455. non-lytic phage titer on X-gal plates -urgent help needed - (reply: 1)
  456. C group Plasmids? - (reply: 2)
  457. large protein degradation - during baculovirus purification (reply: 2)
  458. help with recircularizing a blunt-ended vector? - (reply: 6)
  459. pcDNA3 expression vector is suitable for TNT in vitro? - (reply: 2)
  460. Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
  461. Help! my "plasmid" won't cut. - RE digestion problem (reply: 7)
  462. Help with Cloning into LARGE vector - (reply: 11)
  463. 2 plasmid electroporation. Tips? Advice? - (reply: 5)
  464. stain genomic and plasmid DNA - FACS analysis (reply: 1)
  465. about large-scale preparation of empty vector - (reply: 2)
  466. linear or supercoiled plasmid for creating stable cell line - (reply: 1)
  467. plasmid stability - (reply: 4)
  468. RNA transfection VS Plasmid DNA transfection - (reply: 3)
  469. Has anyone else encounted problems in cloning with PET-22b vector? - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
  470. the plasmid even smaller than the empty PET-22b vector! - the plasmid even smaller than the empty PET-22b vector! (reply: 1)
  471. cloning large inserts in large vectors - (reply: 4)
  472. 2 plasmids in one competent cell - is this possible? - Cloning query (reply: 9)
  473. A plasmid mobility question. - (reply: 3)
  474. Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
  475. shorter vector fragment at the end of the cloning - (reply: 8)
  476. Interaction of Glucose with plasmid DNA - (reply: 3)
  477. binary Ti vectors and Agrobacterium strains - (reply: 1)
  478. Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
  479. Stable cell trasfection questions... - Integration of plasmid... (reply: 5)
  480. Plasmid for qPCR standard curve - (reply: 3)
  481. Plasmid as a PCR template - (reply: 2)
  482. Difference between Extraction of DNA from chromosomal DNA and plasmid DNA - (reply: 1)
  483. clonong into a pET 28 vector - (reply: 2)
  484. Is my plasmid contaminated by RNA? - (reply: 13)
  485. pCMVneo - need a description, company or plasmid map (reply: 2)
  486. CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
  487. why does empty EGFP vector has no fluorescence? :/ - (reply: 5)
  488. dephosphorylated vector and PCR insert - (reply: 7)
  489. 2 PCR product to be inserted into 1 expression vector - (reply: 2)
  490. Uncut plasmid DNA - (reply: 6)
  491. Plasmid DNA Isolation problems, contaminating chromosoma DNA - How to isolate large inserts by miniprep and without chromosomal smearing (reply: 4)
  492. Can I select stable transfectants with amp-resistant vector? - (reply: 3)
  493. Isolation of plasmid DNA - alkaline lysis method (reply: 3)
  494. Isolation of plasmid DNA - chemicals used-why?? (reply: 5)
  495. genomic DNA contamination in plasmid DNA prep - (reply: 1)
  496. How long is too long for a TA vector? - (reply: 2)
  497. plasmids to quantify copies of virus - (reply: 4)
  498. RACE product cloning in TOPO vector - (reply: 1)
  499. 38bp insert and 6.7kb vector ligation problem - the ligation efficiency is too low (reply: 8)
  500. annealing oligo to a cut vector - (reply: 3)
  501. How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
  502. How to store a linearized Vector? - (reply: 7)
  503. Which TA vector do you use? - Who do you use, or do you make your own? (reply: 2)
  504. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
  505. T4 phage DNA isolation protocol - (reply: 1)
  506. Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
  507. Plasmid CpG methylation after transfection - (reply: 2)
  508. plasmid purification from mammalian cells? - (reply: 1)
  509. plasmid purification from mammalian cells? - (reply: 4)
  510. SDS page and gene cloning - pBS plasmid (reply: 1)
  511. Cloning of a Large Insert into a Lentiviral Vector - (reply: 5)
  512. will linearizing plasmid prolong transient transfection ? - (reply: 1)
  513. OH, another unknown vector (oh, sorry I got it finally) - pLysS (reply: 1)
  514. Making your own TOPO vector - (reply: 2)
  515. Request a map of vector pJR-1 - (reply: 1)
  516. Problems with plasmid transfection into primary keratinocytes - (reply: 3)
  517. getting rid of undigested vector - (reply: 5)
  518. Somebody just sent me a plasmid.... - plasmid recovery (reply: 6)
  519. AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
  520. Plasmid stability/Cloning problem - (reply: 3)
  521. reconstituted plasmid DNA solution slightly milky - (reply: 3)
  522. Problems using gentamicin plasmids - (reply: 6)
  523. Insert is double the size than vector - (reply: 6)
  524. Multiple transformation - How many plasmids is it possible to transform at the same time ? (reply: 2)
  525. too confused about plasmid concentration? - (reply: 4)
  526. Problems with Plasmid purification - no real precipitation of genomic DNA (reply: 8)
  527. Plasmid Sequence for pACT1-GFP - (reply: 4)
  528. vector problems - (reply: 2)
  529. which restriction enzyme should I use to digest the vector? - (reply: 3)
  530. digesting pQE30 vector gives inexplicable results - (reply: 3)
  531. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
  532. same vector but diffirent clones produce bands of different size. - (reply: 4)
  533. difference between DH5alpha and lamda pair DH5alpha? - (reply: 2)
  534. Plasmid storage and DNA concentration - (reply: 4)
  535. about using the pLentiLOX 3.7 vector in the lentiviral system - (reply: 6)
  536. shuttle vector - (reply: 1)
  537. Plasmid DNA problem - PEG precipitation (reply: 3)
  538. about MTS assay and plasmid transfection - (reply: 2)
  539. where can I get any plasmid with aadA as selective marker gene? - i might offer some money (reply: 1)
  540. How to make plasmid map? - (reply: 4)
  541. what is IRES?What is it for? - vector construction (reply: 3)
  542. making a vector construct and transfecting in smooth muscle cells - (reply: 6)
  543. Will linearized plasmid still have drug resistance? - (reply: 13)
  544. Cloning and Expression vectors - Which is the best Expression vector to use? (reply: 3)
  545. cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
  546. possible contamination of TA cloning vector? - (reply: 4)
  547. Contamination with TA cloning vector? - (reply: 1)
  548. Transformation efficiency of plasmid dna - (reply: 2)
  549. Supercoil migrates slower than linear plasmid? - (reply: 2)
  550. vector for expression of tag on cell surface - (reply: 7)
  551. Transformation of a plasmid into E.coli BL21 - problem! - Unexpected bands (reply: 7)
  552. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
  553. Express miRNA in vector for gene knockdown - (reply: 4)
  554. strange digested vector isolation - (reply: 7)
  555. Map of MIGR1 retroviral vector needed - (reply: 1)
  556. why competent cells take up only 1 type of plasmids? - (reply: 7)
  557. pXT7 vector map - (reply: 3)
  558. Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
  559. baculovirus expression system - (reply: 3)
  560. pGEM T easy vector for cloning - (reply: 29)
  561. about mutant plasmid construction - (reply: 3)
  562. How to get suicide plasmid? - (reply: 1)
  563. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
  564. protein expression in pET-30a vectors - (reply: 2)
  565. Ligation problem, might be bc/ it's the 5kb insert? - Vector and insert are pretty much same size.. and it's not workin in a ' (reply: 3)
  566. who can recommend me a good baculovirus expression system? - (reply: 3)
  567. Inserting tag into the vector. - where is IRES? (reply: 11)
  568. Regeneration of plasmid preparation columns - Has anyone a protocol? (reply: 1)
  569. MIGR1 RETROVIRAL VECTOR - (reply: 2)
  570. ligation never works even for single cut vector!Why so!Help! - (reply: 7)
  571. two plasmids of the same protein with tags on N and C-terminus. - how can I make use of them? (reply: 3)
  572. Block-it Plasmid Ligation - (reply: 8)
  573. vector for stem cell differentiation - (reply: 2)
  574. shRNA Plasmid propagation in Xl1 Blue - (reply: 1)
  575. E-coli is eating up my plasmid! - (reply: 9)
  576. pECL Eco viral vector and Rat cells - viral receptor present? (reply: 1)
  577. inserting shRNA vs full gene into vector ? - (reply: 10)
  578. Problems with inverse PCR - Identification of gene trap vector insertion (reply: 3)
  579. advice on construct - possible problem with vector (reply: 7)
  580. any good method for rapid screening for recombinant plasmids? - (reply: 5)
  581. E-coli strain and plasmid incompatibility...? - is there something like that? (reply: 14)
  582. vector PCR problem - stuck in experiments... (reply: 2)
  583. Can all bacterial plasmids work in plants too? - (reply: 6)
  584. Cloning Construction of 9000bp vector with 900bp - (reply: 14)
  585. Lambda phage assay - (reply: 2)
  586. Mutagenesis Help (plasmid isolation & sequencing stages) - (reply: 2)
  587. MIGR1 retroviral vector transfection - (reply: 3)
  588. plasmid construction - (reply: 1)
  589. Can I still use pGEFP-C1 vector? - (reply: 2)
  590. Amplify retroviral vectors doesn't work from miniprep to midiprep - (reply: 6)
  591. Cloning WITHOUT Topoisomerase! - Does any one know of an expression vector that does not depend on Topoisomerase (reply: 13)
  592. cloning problem with pcr and pegfp vector - (reply: 9)
  593. problems with cloning into a pET15b vector - (reply: 2)
  594. Religation with single digest vector - (reply: 7)
  595. Asking help for purifying plasmids - (reply: 9)
  596. Asking help for purifying plasmids - (reply: 3)
  597. Added E coli to plasmid by mistake - How to get plasmid back? (reply: 7)
  598. vector for express secreted protein in vivo - (reply: 3)
  599. VECTOR LIGATION WITH One RE - (reply: 4)
  600. ires... - IRES IN VECTORS.. (reply: 3)
  601. dimer in vectors - (reply: 2)
  602. LOOKING for vector map and sequence of pSVaZ11 - (reply: 1)
  603. His-Tag Question (pQE30/31/32 Qiagen Vector) - (reply: 2)
  604. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
  605. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
  606. plasmid isolation from a transient transfection - (reply: 2)
  607. Should I purify digested vector before ligation? - (reply: 7)
  608. Vector NTI's local database - (reply: 2)
  609. plasmid DNA running on agarose gel to the opposite direction - (reply: 7)
  610. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
  611. how the gene cloned into a vector can expressed in mammalian cell without the 5& - What is the mechanism of translation initiaition with the HCM promoter? (reply: 4)
  612. Linealizated plasmid for stable transfection? - is that right? (reply: 1)
  613. Does it need cotransfection vector when retrovirus transfected hek293? - (reply: 7)
  614. maximum plasmids can be co-transfected into Hela cells - (reply: 4)
  615. Plasmid instability - why and how do i check? (reply: 1)
  616. Plasmid cotransfection for selection - (reply: 4)
  617. Cloning a very small insert into a "giant" vector! - (reply: 8)
  618. transformation of competent cells with recombinant plasmids - (reply: 2)
  619. plasmids smaller than empty vector - using expensive cells - still not working (reply: 1)
  620. checking colonies by vector primers.... - (reply: 7)
  621. Gateway vectors - (reply: 1)
  622. trouble ligating fusion-PCR into vector - (reply: 6)
  623. Any body knows the characters of plasmid pINT007 - (reply: 4)
  624. why do supercoiled plasmid transform faster than linear plasmid - (reply: 3)
  625. Plasmid isolation - (reply: 2)
  626. help in Plasmid 'mini-prep' experiment - (reply: 5)
  627. plasmid DNA precipitation - with the use of PEG-MgCl2 (reply: 1)
  628. Vector: pIRES - (reply: 4)
  629. inducation with lamda CE6 - (reply: 1)
  630. insert to vector ratios for ligations - what do y'all do? (reply: 4)
  631. endotoxin free plasmid preparation - kits comparable? (reply: 2)
  632. cleaning up plasmid preps - (reply: 6)
  633. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  634. Amplification of phage library - (reply: 3)
  635. p2T7 Vector Sequence - (reply: 1)
  636. Insert 2 single strand DNA into a vector - (reply: 6)
  637. disappearing vector after digestion - (reply: 11)
  638. Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
  639. Removing vector restriction sites - and replacing with new ones (reply: 8)
  640. Stuborn band after digestion of plasmid DNA - (reply: 4)
  641. How to remove endotoxin? - want a endotoxin-free plasmid for transfection (reply: 5)
  642. Vector problems? - (reply: 7)
  643. Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
  644. Calculating insert/vector ratio - (reply: 7)
  645. methylated plasmid - (reply: 3)
  646. Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers (reply: 1)
  647. qPCR Standard control - plasmid quantify (reply: 3)
  648. PEG precipitation of phage - (reply: 3)
  649. plasmid DNA and restrictions enzyme - (reply: 6)
  650. Cloning Hell - problems with aquiring insert into vector (reply: 5)
  651. Plasmid Purification - Any tricks to increase yield? What could I be doing wrong? (reply: 2)
  652. is there a restriction about amount of vector in a ligation? - (reply: 14)
  653. To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
  654. cloning in a long vector (~10kb) without sequence changes - (reply: 2)
  655. does phenol:chloroform extracted plasmid creats problem in Mammalian cell cultur - (reply: 5)
  656. re-dephosphorylation of vector? - (reply: 3)
  657. TA cloning...why is there no plasmid for BLUE colonies? - (reply: 2)
  658. plasmid miniprep question - (reply: 4)
  659. Is competent cell still good in -80oC for 16 months? - got a different plasmid after plasmid prep (reply: 3)
  660. Electrophoresis of Plasmid - (reply: 3)
  661. Vector question - (reply: 6)
  662. Working with phage( T4 bacteriaphage) - several questions abt phage (reply: 3)
  663. pSM2C retrovirus vector from Openbiosystems, is it good? - (reply: 5)
  664. Y2H vectors compatible? - (reply: 1)
  665. How to choose a suitable phage display system - (reply: 1)
  666. What insert:vector ration is best? - (reply: 18)
  667. Plasmid map of the clone with APE or DNA Strider - (reply: 2)
  668. anyone heard of pEBB vector? - or where to find info? (reply: 4)
  669. Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
  670. help with transfection of large size plasmid - (reply: 2)
  671. isolation of phage dna - isolation of phage dna (reply: 1)
  672. plasmid size change after transformation - (reply: 1)
  673. Methods for concentrating plasmid DNA - (reply: 23)
  674. BAC Vector with SalI in MCS? - (reply: 1)
  675. really lost in plasmid ligation - urgent! (reply: 6)
  676. Making blunt and stick end vector - (reply: 2)
  677. Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
  678. why dephosphoration of vector is not recommended? - (reply: 3)
  679. how to move for ligation with low conentration of digested plasmid - (reply: 2)
  680. plasmid in 1M tris ..URGENT - (reply: 1)
  681. pEBB or pEBG vector maps - (reply: 2)
  682. do plasmids amplify more efficiently than genomic DNA? - (reply: 3)
  683. What amount of insert:vector should I use if I want a 3:1 molar ratio? - (reply: 2)
  684. How to calculate insert:vector ratio? - (reply: 2)
  685. plasmid amplification - exons and primers (reply: 1)
  686. plasmid sequencing - (reply: 4)
  687. structure difference between plasmid & chromosomal DNA - (reply: 1)
  688. plasmid DNA dilution for absolute quantitation - (reply: 2)
  689. Peg not precipitating out all the phage - (reply: 2)
  690. Transformation using linearized plasmid? - (reply: 6)
  691. Control Vector - (reply: 4)
  692. My plasmid purity changes with different dilutions - (reply: 1)
  693. Vector searching - (reply: 1)
  694. Plasmid PCR - (reply: 5)
  695. lamda DNA digested with HindIII - (reply: 1)
  696. Plasmid map for ptrc 99c - (reply: 1)
  697. Protocol for RNase treatment of plasmid for RNA cleanup - (reply: 4)
  698. No success with PCR cloning of a gene into expression vector - (reply: 5)
  699. miRNA overexpression - pol II - type vectors... (reply: 1)
  700. low plasmid dna from ET host - Low plasmid Dna concentration in Ecoli ET 12567 (reply: 3)
  701. Expression vector - (reply: 1)
  702. Plasmid Extraction from a big volume of pellet - Molecular biology (reply: 2)
  703. keep getting incomplete digest for plasmid - (reply: 3)
  704. Transformation of a 21kb vector - (reply: 11)
  705. Plasmid DNA purification - (reply: 3)
  706. about antibiotic in plasmid transformation - which is best? (reply: 6)
  707. trouble with methlase sensitve restriction digests on plasmids isolated from DAM - (reply: 2)
  708. Co-transfection for stable cell lines - mammalian - Need to know the ratio of the plasmids that need to be used (reply: 2)
  709. plasmid contamination - (reply: 2)
  710. Is re-sequencing needed for insert ligated into a second vector? - (reply: 1)
  711. Linear or unlinear plasmid before transfection for expression? - (reply: 2)
  712. Homology of plasmid sequence - Is Origin of relication sequence lifted from the bacterial genome? (reply: 2)
  713. direct plasmid sequencing problems - direct plasmid sequencing problem (reply: 4)
  714. how to co-transfect different plasmids? - (reply: 3)
  715. How to get a good quality of plasmid in maxi prep. - (reply: 7)
  716. How to extract a 20kb vector after double digestion - (reply: 4)
  717. in vitro translation vector - (reply: 3)
  718. To make good plasmid for expression transformation - (reply: 1)
  719. retroviral vector - [help] pSM2c and LinX (reply: 2)
  720. inserts and vector ratio in liagtion Rxns - (reply: 6)
  721. Troubles with vector dephosphorylation - troubles with vector dephosphorylation (reply: 2)
  722. transiently transfected vectors replication - do they replicate in an eukaryotic cell? (reply: 1)
  723. Isolation of episomal plasmid DNA from mammalian cells - (reply: 1)
  724. plasmid contaminated with another dna - (reply: 1)
  725. plasmid sterilization - (reply: 1)
  726. Dual Vector backbones - cloning problems? (reply: 1)
  727. can you introduce mutation directly into the cells? - other than just transfecting it with a vector... (reply: 1)
  728. Lentiviral vector production timeline - (reply: 1)
  729. How to spot plasmid on paper for shipping? - (reply: 4)
  730. Do transfected vectors bind histones and/or methylate? - (reply: 10)
  731. Plasmid DNA purification - Lithium chlorid (reply: 1)
  732. How to elute plasmid spoted on paper - (reply: 3)
  733. stability of TOPO vector at RT - (reply: 3)
  734. About the procedure of plasmid linearization - How many positions and the proper position? (reply: 2)
  735. ligating adaptor in 26kb plasmid - (reply: 1)
  736. No plasmid in HB101 - whereas DH5alpha does (reply: 2)
  737. Plasmid purification - (reply: 5)
  738. does anyone have the pLSA-beta-geo vector map? - (reply: 6)
  739. plasmid rescue method - (reply: 3)
  740. pCMV-CD19 plasmid map - (reply: 2)
  741. Transduction using lentiviral vector - (reply: 3)
  742. Do you have any problem with QIAGEN Plasmid Maxi kit - (reply: 5)
  743. about siRNA and shRNA - RNAi Vector (reply: 2)
  744. pMT2 vector sequence - (reply: 1)
  745. Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
  746. RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
  747. good vectors for neuronal expression - (reply: 13)
  748. Where to find list of plasmid copy numbers? - I need to know approximate copy number before I choose vector (reply: 5)
  749. how to recognize destination vector in vector NTI - (reply: 1)
  750. gel purification of the vector... - is it good enough for ligation? (reply: 7)
  751. Plasmid Concentration Calculation - (reply: 9)
  752. How to accurately quantify plasmid DNA in solution within impurity of RNA? - (reply: 3)
  753. not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
  754. purification of digested plasmids - (reply: 4)
  755. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
  756. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
  757. Another Problematic Ligation - 110bp ligation into 2.5kb vector (reply: 7)
  758. pBABE plasmid - (reply: 3)
  759. Need Help ASAP with maxiprep - Diluted plasmid with buffer ER instead of TE (qiagen kit) (reply: 3)
  760. isolation of DNA from a plasmid for site-directed mutagenesis? - do researchers actually do this? (reply: 6)
  761. is it my recombinant plasmid or contamination? - only two colonies on the plate.... (reply: 5)
  762. transformation of a big plasmid in E. coli - something wrong is happening in my transformation (reply: 6)
  763. plasmid map software? - (reply: 7)
  764. cloning vector - cloning vector (reply: 1)
  765. which vector will be best for cloning and then expression - vector (reply: 1)
  766. pBR322 relaxed plasmid....what type of media? - (reply: 3)
  767. Anyone has the experience to order plasmid from "Addgene"? - (reply: 2)
  768. Plasmid appears as two bands after double digestion - (reply: 2)
  769. How to insert one single nucleotide into a plasmid? - (reply: 6)
  770. Side directed mutagenesis with 17kb plasmid - (reply: 19)
  771. chloroform v. phenol/chloroform/isoamylalcohol extraction of plasmid DNA - advantages/ disadvantages of each? (reply: 4)
  772. Differenciation between plasmid, super-coiled DNA and linear DNA - What is the difference between plasmid and other DNA on the gel? (reply: 1)
  773. RNase treatment of plasmid - (reply: 7)
  774. Mammalian expression vector - (reply: 3)
  775. Plant expression vector - (reply: 4)
  776. ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
  777. Do TetO seqeunces make plasmid difficult to grow? - (reply: 2)
  778. Transfering a DNA construct from one vector to another - in frame/not in frame? (reply: 2)
  779. is the polylinker in pUC18 plasmid a artificially inserted fragment? - want to know whether the polylinker site is a inserted or naturally present in t (reply: 1)
  780. Phage precipitation techniques? - (reply: 1)
  781. Miniprep plasmid shows three bands - (reply: 1)
  782. Expression problem in pQE30 vector system - (reply: 2)
  783. clarification - plasmid and gel - (reply: 9)
  784. Problems cloning into pET Kanamycin resistance vectors - (reply: 7)
  785. vector with tag recommendation - (reply: 10)
  786. Vector databases and resources - (reply: 6)
  787. pCIS expression vector - (reply: 2)
  788. Baculovirus Expression - Sonicate infected cells? (reply: 2)
  789. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
  790. vector sequence - (reply: 3)
  791. non-expression vector - (reply: 6)
  792. Vector insertion for beginners - (reply: 4)
  793. Ligation help needed! - vector religation? (reply: 3)
  794. plasmids sequences - plasmids sequences (reply: 1)
  795. No insert for cloning PCR product into expression vector - (reply: 5)
  796. plasmid incompatibility in mammalian cells? - (reply: 1)
  797. Maxiprep of plasmid DNA - (reply: 1)
  798. vectors (PJG4-4, PJG4-6) - maps of PJG4-4, and PJG4-6 vectors (reply: 2)
  799. pCG plasmid - (reply: 2)
  800. Ligation is killing me - 1kb insert, 6 kb vector (reply: 11)
  801. failure of insertion of fragment into large vector - (reply: 1)
  802. pcDNA3.1 & pBIG2i vectors appear "bigger" on the gel! :( - (reply: 1)
  803. antibiotics for 3 vectors and sequencing - (reply: 2)
  804. RT-PCR on plasmid - (reply: 1)
  805. How to make a T/A vector - (reply: 2)
  806. which tag and vector is the best for expressing protein in bacteria? - (reply: 4)
  807. Two same oris (origins of replications) in one plasmid - (reply: 1)
  808. Best Vector for shRNA production - Help (reply: 5)
  809. how to isolate plasmid insert? - (reply: 1)
  810. Retroviral Vector won't digest ideas! - (reply: 1)
  811. Kozak Sequences and Mammalian Expression Vectors - (reply: 2)
  812. what would happen if two SV promoter show in one vector - (reply: 1)
  813. HIS-TAG LIGATION - Ligation of His-tag into the pIMS vector (reply: 2)
  814. Vector self ligation? - (reply: 1)
  815. GW1 plasmid sequence - someone know this plasmid???? (reply: 1)
  816. nonspecific cellular responses - vector system vs synthetic siRNA - (reply: 1)
  817. yeast plasmid as template - (reply: 1)
  818. vector map - (reply: 4)
  819. cloning systems - pGEM-T easy vector system versus TOPO TA cloning? (reply: 4)
  820. ligating small oligos - how to ligate small oligos into vector (reply: 2)
  821. vector basics for transgenic mice - (reply: 2)
  822. Mystery band after vector digestion - (reply: 3)
  823. putting BAC plasmid back into E. coli - (reply: 7)
  824. destabilized EGFP Vector urgently needed - (reply: 4)
  825. Long PCR for amplification from plasmid - (reply: 5)
  826. sequencing with large plasmids - (reply: 4)
  827. Why freshly prepared alkaline solution II for plasmid extraction? - (reply: 10)
  828. How to make a point mutation in a wild type plasmid. - (reply: 2)
  829. Restriction analysis of a vector - (reply: 17)
  830. S. aureus plasmids - S. aureus plasmids (reply: 1)
  831. Crude Plasmid Digests - Help! (reply: 8)
  832. Purify genomic from plasmid DNA - (reply: 5)
  833. problems with digesting insert from vector - (reply: 4)
  834. three way ligation (two fragment and a vector) - (reply: 1)
  835. how to amplify fragment from the plasmid - (reply: 3)
  836. Do you have to linearize your vector before transfection? - (reply: 4)
  837. How to remove small amount of plasmid from filter paper - (reply: 6)
  838. stable transfection of two plasmids? - (reply: 4)
  839. a problem of pCMV-Fluc plasmid transfection. - (reply: 1)
  840. PCR an insert in a vector with wrong product size - (reply: 1)
  841. A few questions about insertion into a plasmid - (reply: 7)
  842. phosphorylated oligos and vector - (reply: 2)
  843. how to amplify a 350bp in length from a vector - (reply: 2)
  844. transfection of HiFive Cells? - Baculovirus Expression (reply: 1)
  845. Vector Construction- Tagging N-term - necessity of ATG in Gene of Interest (reply: 2)
  846. vector with H1 promoter be transfered to mouse cells? - (reply: 3)
  847. Restriction enzyme SBFI failed to cut plasmid - (reply: 3)
  848. Calculate insert/vector ratio for ligation - (reply: 8)
  849. How can I change a promoter of a plasmid? - (reply: 1)
  850. Concentrating plasmid DNA - (reply: 3)
  851. Cloning issue - vector insert ratio - (reply: 3)
  852. promoter selection in plasmid based shRNA - (reply: 2)
  853. help:failure of extraction of plasmid DNA - (reply: 2)
  854. Designing siRNA expression vectors - (reply: 2)
  855. Expression problems with pIRES vector from Clontech - EGFP at MCSB site cannot be expressed! (reply: 1)
  856. About RNAi based on sh-RNA plasmid - (reply: 2)
  857. SV40-less Luciferase promoter plasmid? - (reply: 2)
  858. How to predict a promoter region for a gene - Promoter cloning + genes into vector (reply: 6)
  859. Promoter insertion in pUAST vector - Promoter insertion in pUAST vector (reply: 1)
  860. TA cloning Vector - ...how to prepare it myself (reply: 1)
  861. cannot isolate plasmid from mutants - please HELP! (reply: 1)
  862. i need help : lambda phage excision problem !! - labmbda phage excision problem (reply: 4)
  863. Can't get rid of RNA in my phage extraction. - (reply: 4)
  864. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
  865. Storing low conc plasmid DNA - (reply: 3)
  866. problems cloning into plenti6/V5 lentiviral vector - (reply: 2)
  867. Who knows and uses the Plasmid pGag-PolGpt? - Poor plasmid yield with Maxi-Prep.... (reply: 1)
  868. TOPO TA Vector Cloning problem - no colonies - (reply: 5)
  869. Why my insert and vector dissappear after mini prep? - (reply: 4)
  870. Who knows the plasmid M13? - looking for the plasmid map of the m13 plasmid (reply: 1)
  871. DNA vaccine vectors - (reply: 1)
  872. By using a plasmid (interested insert) as a templete for PCR - PCR (reply: 2)
  873. what's wrong? Plasmid digestion produce only smear - (reply: 6)
  874. How to amplify the insert from a plasmid? - (reply: 2)
  875. TA Vector to pQE Vector chang problem - Can't get rid of originally TA vector.. (reply: 1)
  876. No Plasmid digestion - Enzymes don't cut my miniprep Plasmid-DNA (reply: 2)
  877. Sequencing plasmid gets overlapping peaks - troubleshoot (reply: 5)
  878. Plasmid digestion - Problem with digestion (reply: 3)
  879. plasmid transfection and virus infection simutaneously - (reply: 2)
  880. SDM large plasmid - (reply: 1)
  881. Problem amplifying 3 kp fragment from a plasmid - (reply: 4)
  882. How would one go about moving a gene from plasmid to plasmid - cloning (reply: 2)
  883. Large scale purification of large size plasmid - (reply: 1)
  884. Transfect/Infect hematopoietic cells - What vector systems to use? (reply: 1)
  885. Plasmid miniprep problem - (reply: 4)
  886. Help: anyone who has co-transfected TWO different plasmids - - how much of each DNA to use? (reply: 5)
  887. Plasmid template preparation for in vitro transcription - (reply: 9)
  888. Plasmid - Choosing a plasmid (reply: 2)
  889. non-viral vectors - (reply: 1)
  890. Plasmid changes after transformation - (reply: 2)
  891. dephosphate the digested vector - (reply: 4)
  892. Mysterious gain in vector size after cloning! - (reply: 1)
  893. plasmid DNA isolation - drying dna samples after isop or 70% EtOH wash (reply: 4)
  894. Ligation of two oligos into a vector - (reply: 4)
  895. digestion problem - incomplete digestion of transformed plasmid (reply: 1)
  896. Growing large plasmid (20kb) in e.coli - Low plasmid yield from large construct (reply: 7)
  897. plasmid DNA isolation - problem in plasmid DNA isolation (reply: 3)
  898. Expression Vector Construct - sequence entire insert? (reply: 3)
  899. Identification of the cut and uncut plasmid on gel - (reply: 4)
  900. Dual-promoter plasmids? - Trying to transcribe sense and antisense strands.. (reply: 1)
  901. Inducable mammalian expression vector - (reply: 3)
  902. Is it ok to store a digested vector for ligation? - (reply: 4)
  903. should we dilute the DNA (vector and insert), before ligation? - ligation problem (reply: 5)
  904. How does plasmid DNA miniprep kit get rid of chromosome DNA? - (reply: 4)
  905. Clontech pEGFP-N1 and -C1 vectors - (reply: 4)
  906. Plasmid wash in E. coli? - How to promote the lost of a plasmid in coli (reply: 2)
  907. any effect if the plasmid got 3 gen resistance? - (reply: 1)
  908. no protein expression after induction using pET vector - (reply: 4)
  909. Vector Map Software - Know of any free or cheap? (reply: 8)
  910. Protease in expression vector - (reply: 3)
  911. Trouble cloning a 8.5 kb fragment into a pTRE vector - (reply: 2)
  912. Ligation of 2 fragments and one vector - (reply: 7)
  913. Rolling circle replicating plasmid and transformation - Gene cloning (reply: 2)
  914. Maximum insert size in a pGEM Teasy vector - (reply: 2)
  915. Construct DNA plasmid for QPCR - (reply: 4)
  916. Plasmid preparation problem using alkaline-lysis - (reply: 5)
  917. Putting 2 plasmids in 1 e. coli cell - (reply: 4)
  918. antibiotics concentration for ligated plasmid - (reply: 3)
  919. circular vector propagation - How? (reply: 1)
  920. No colonies with site-directed mutagenic plasmids - (reply: 2)
  921. pSUP5011 - what is the molecular size of the plasmid pSUP5011 (reply: 1)
  922. plasmid extraction and antibiotics matters... - (reply: 4)
  923. PCR on plasmids? - (reply: 2)
  924. Plasmid methylation problem - Plasmid methylation (reply: 3)
  925. Linearized or unlinearized plasmid for stable transfection? - (reply: 18)
  926. Self ligation of vector - (reply: 2)
  927. TA cloning - pGEMT easy vector system-not a single colony (reply: 6)
  928. wash off plasmid in the tube for reuse - question (reply: 1)
  929. pSVT7 vector sequence or map - (reply: 3)
  930. real-time pcr plasmid standards - (reply: 4)
  931. Tansfection in drosophila S2 cells with pEGFP-N1 - pEGFP-N1 vector @ drosophila S2 cells (reply: 1)
  932. PCR of plasmid miniprep from Agrobacterium - Can't get PCR product out of plasmid miniprep (reply: 1)
  933. Whatman Filter Paper - Elution of plasmid DNA (reply: 1)
  934. How to construct T vector - (reply: 8)
  935. transfecting siRNA and a plasmid - (reply: 4)
  936. plasmids turnedinto smears after NcoI digestion - restriction enzyme problem (reply: 1)
  937. Plasmid sequencing - (reply: 2)
  938. plasmid sequence - (reply: 3)
  939. Why ligation reactions are done at 4C - Ligation of insert into vector (reply: 2)
  940. poor ligation of plasmid/oligo construct - (reply: 8)
  941. Stable Transfections - do you NEED to linearise the plasmid? (reply: 1)
  942. Taking OD of plasmid dsDNA in ddH20 - plasmid prep (reply: 3)
  943. plasmid stock - (reply: 6)
  944. How to dissolve over-dried DNA? - plasmid prep (reply: 6)
  945. Plasmids in eluting buffer - (reply: 1)
  946. pGEM-T easy vector for expression - (reply: 6)
  947. Storing of miniprep or maxiprep plasmids - (reply: 7)
  948. PCR an insert in a vector with wrong product size - I can not amplify my fragment with a normal PCR (reply: 8)
  949. vector auto ligation problem - (reply: 1)
  950. mammalian expression vector - (reply: 1)
  951. Human infection from Viral Vectors? - (reply: 4)
  952. why linearize plasmids make transfection stable? - (reply: 6)
  953. ligation problem - self ligation of vector - (reply: 5)
  954. Blunting both vector and insert - klenow and CIPping (reply: 2)
  955. problems purifying plasmid DNA after restriction digestion - (reply: 3)
  956. Agarose Gel Electrophoresis of plasmid DNA - Agarose Gel Electrophortesis of plasmid DNA (reply: 1)
  957. virus vector for transfection - (reply: 8)
  958. making more plasmid - (reply: 2)
  959. Vector with T5-promotor in Bl21/DE3-system - Has anyone had experience of the T5-promotor being leaky? (reply: 1)
  960. Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a (reply: 1)
  961. How big DNA fragment can I clone to pUC19 plasmid? - (reply: 2)
  962. protein expression - pkk223-3 vector (reply: 3)
  963. Lentiviral vector for infecting primary cells? - Any suggestion for a suitable vector? (reply: 1)
  964. expression vector pHM6's map - (reply: 3)
  965. de-phosphorylating a vector - (reply: 7)
  966. Unstable plasmid ?? - Deletions only after ligation ! (reply: 2)
  967. The amount of plasmid for ligation reaction - Help (reply: 2)
  968. transfection of single copy of plasmid per cell - (reply: 3)
  969. AB resistance gene - Which antibiotic resistance gene in my vector ? (reply: 3)
  970. Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
  971. Plasmid DNA cleaning - (reply: 2)
  972. Sequencing of Plasmids - (reply: 2)
  973. Which vector for cloning big PCR fragments? - (reply: 3)
  974. About identify the inserted gene? - a using PCRII- topo vector question (reply: 4)
  975. Dephosphorylation of vector before ligation - Is it required? (reply: 38)
  976. Plasmid drawing tools - User friendly Plasmid drawing tools (reply: 1)
  977. Plasmid DNA contamination - the source of Chrosomal DNA contamination (reply: 2)
  978. Plasmid DNA Purification - plasmid was contaminated by Genomic DNA (reply: 1)
  979. Cloning into shRNA vectors - (reply: 4)
  980. Vector Vs Host - PGL2 Vs DH5alpha (reply: 1)
  981. How to prepare enough plasmid for transfection? - (reply: 3)
  982. Cloning into GFP vector - (reply: 2)
  983. Plasmid DNA storage - (reply: 4)
  984. purify linearized plasmid for transfection - (reply: 1)
  985. question about determinating the size of plasmids - (reply: 2)
  986. question about plasmid linearization - (reply: 2)
  987. about vectors and transfection of RNAi - (reply: 1)
  988. Low copy number of plasmid - (reply: 3)
  989. Can shRNA be transfected without vector...?? - Planning new experiments (reply: 3)
  990. plasmid restriction map- free software - (reply: 3)
  991. Help: baculovirus expression system? Anyone's used it? - Has anyone used this system (reply: 1)
  992. Bad OD ratios from Plasmid Minipreps - (reply: 2)
  993. cloning problem - cloning problem in GFP vector (reply: 4)
  994. Problem cloning PCR product into double-cut vector - (reply: 3)
  995. RNAi vector for stable expression - (reply: 4)
  996. Plasmid gene mutation - (reply: 1)
  997. sucide plasmid directed mutagenesis - sucide plasmid directed mutagenesis in pseudomonas (reply: 1)
  998. Need help in plasmid sequencing - (reply: 4)
  999. Subcloning into large plasmid - (reply: 3)
  1000. plasmid digest - restriction enzyme won´t cut (reply: 1)
  1001. ligation problems and searh for map of pGW1 vector - (reply: 1)
  1002. Freezer culture fails to retain plasmid - (reply: 3)
  1003. doing phage titrering - (reply: 1)
  1004. help for large-scale plasmid purification - (reply: 3)
  1005. Suspicion of Phage infection - Urgent, pls help (reply: 2)
  1006. T- vector clone - (reply: 3)
  1007. Does my bacteria have a plasmid? - (reply: 5)
  1008. Subcloning into lentiviral vectors - Very little to no plasmid after miniprep (reply: 7)
  1009. DNA plasmid extraction trouble - (reply: 1)
  1010. Software for plasmid drawing - (reply: 1)
  1011. Plasmid DNA purification - DNA purification (reply: 7)
  1012. cloning PCR fragment - PCR fragment is not getting cloned in the vector (reply: 2)
  1013. removing plasmids from yeasts - (reply: 1)
  1014. How to ligate ds-oligos into a vector? - (reply: 48)
  1015. transformation - Possible to get two diff. plasmids? (reply: 1)
  1016. wierd plasmids: maxi-prep never work - (reply: 13)
  1017. plasmid draw - Need software for Vector cloning design (reply: 4)
  1018. Problem with plasmid linearization - (reply: 2)
  1019. Plasmid extraction from E.coli culture - (reply: 7)
  1020. Promoterless Retroviral vector? - (reply: 1)
  1021. plasmid went missing - what happen?? (reply: 5)
  1022. vector pFPV25.1 - need help!!! (reply: 2)
  1023. Quickchange site directed mutagenesis - Success with a 13 kb plasmid? (reply: 2)
  1024. stable transfection with siRNA expression vector - (reply: 6)
  1025. Shipping of plasmids and X-rays - (reply: 1)
  1026. the vector for stable transfection - (reply: 3)
  1027. plasmid sequence - IncQ (reply: 1)
  1028. vector map - (reply: 2)
  1029. vector sequence - pBABE vector sequences (reply: 7)
  1030. the host cell of plasmid:pBmIEGLacZ-BmIEGhyg - (reply: 1)
  1031. how to add flag to a vector - (reply: 7)
  1032. blunt-cloning - all are vector self-ligation products? (reply: 33)
  1033. 42bp fragment ligation to 4776bp vector - (reply: 3)
  1034. Cloning of high-GC% gene into vector - (reply: 3)
  1035. Big Size of Plasmid Transformation - Urgent! (reply: 1)
  1036. Site directed mutagenesis - Of 20 kb plasmid (reply: 1)
  1037. plasmid for stable transfection - (reply: 1)
  1038. Plasmid problem - (reply: 2)
  1039. Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
  1040. Plasmid DNA extraction - Extracted plasmid cannot be seen in agarose gel (reply: 9)
  1041. RNA  in plasmid preparation interfere in ligation - RNA contamination and ligation (reply: 3)
  1042. phage PCR - (reply: 1)
  1043. How do I extract a HUGE plasmid? - (reply: 1)
  1044. Expression vector/host for plant genomic sequence - (reply: 3)
  1045. plasmid mini-prep from E.coli - smeared bands (reply: 2)
  1046. plasmid can't be digested by enzyme - (reply: 13)
  1047. Plasmid Extraction Kits Manufactured by? - Plasmid Extraction Kits Manufacture (reply: 1)
  1048. how to identify whether the PUC18 plasmid is good or not - about the PUC18 (reply: 1)
  1049. Transformation technique's and [abx] affect on plasmid yield - plasmid production and isolation (reply: 1)
  1050. Vector Suggestion needed - (reply: 1)
  1051. Met trouble in ligation! - insert:sacII,XhoI;vector:sacII,SalI (reply: 1)
  1052. How to transform the cells witht wo different plasmids - (reply: 3)
  1053. recover a plasmid about 50kb with high yield? - (reply: 1)
  1054. cDNA library (in Lambda  ZAP vector) screening - picking up plaques on nitro cellulose  membrane as replica (reply: 2)
  1055. Vectors - (reply: 1)
  1056. ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
  1057. M13 phage amplification,who canhelp? - (reply: 2)
  1058. anybody can provide me with aplasmid vector which can be visually scre - (reply: 3)
  1059. Where is a good vector databaseon the internet? - (reply: 1)
  1060. Prk5 plasmid - mammalian experssion vector (reply: 1)
  1061. molecular biology - cloning into pPic9 vector (reply: 1)
  1062. BAC vector problems - (reply: 1)
  1063. Removal of Rna from plasmid prep - (reply: 1)
  1064. Plasmid prep doesn't work! - Help needed with HB101 strain plasmid prep (reply: 3)
  1065. question about plasmid digested by one-cut-site RE - (reply: 3)
  1066. Co-transfection Question - Will co-transfected vectors enter the same cell? (reply: 2)
  1067. ligate 3 DNA fragments with sticky ends into Vector - (reply: 1)
  1068. Yeast plasmid extraction - (reply: 5)
  1069. plasmid isolation - (reply: 6)
  1070. Plasmid isolation again - Isolation from multiple colony? (reply: 1)
  1071. Have anyone ever used this plasmid? - (reply: 2)
  1072. Re: How to transform thecells with two different plasmids with d... - (reply: 1)
  1073. trouble in plasmid pcr - i cannot obtain designed band from a definied plasmid (reply: 4)
  1074. how to isolate huge plasmid DNAfrom Pseudomonas putida - (reply: 2)
  1075. Plasmids purification - how to remove DNA contamination (reply: 3)
  1076. How to clone large DNA fragment into small vector! - (reply: 1)