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dephosphorylated vector - (Jul/10/2007 )

How long dephosphorylated and gel eluted vector can be kept in freezer and reused for cloning.

-samita-

if you did your work cleanly, especially if the DNA concentration was initially high, then I'll say the fragments should last a year, if not more. (I have an insert prep that was cut 2yrs ago and that still ligates fine. A labmate used depho vector which was 2yrs old and that ligated okay too).

-perneseblue-