ligating adaptor in 26kb plasmid - (Feb/03/2006 )
Dear all..
Im having a problem to ligate an adaptor of Swa 1 site into a plasmid size 26 kb..
The adaptor is about 35 bp only..
im in despair and desperately need the adaptor to be ligated..what is the best ratio should i do to ligate it?
thanks..
-whimsicalDNA-
QUOTE (whimsicalDNA @ Feb 3 2006, 02:28 AM)
Dear all..
Im having a problem to ligate an adaptor of Swa 1 site into a plasmid size 26 kb..
The adaptor is about 35 bp only..
im in despair and desperately need the adaptor to be ligated..what is the best ratio should i do to ligate it?
thanks..
Im having a problem to ligate an adaptor of Swa 1 site into a plasmid size 26 kb..
The adaptor is about 35 bp only..
im in despair and desperately need the adaptor to be ligated..what is the best ratio should i do to ligate it?
thanks..
Have you tried to CIP your plasmid? Because if you do, you may succeed with very little adaptors compared to the CIPped plasmid.
-pjansen-