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eukaryotic inducible vector with GFP-tag fusion? - (Nov/05/2008 )

beside the inducibility of gene expression in mammalia cell line cells, the vector should also produce a fusion to a fluorescent protein such as GFP... any suggestions?

-The Bearer-

Wouldn't it be simpler to produce the fusion protein yourself, and then find an inducible vector to put it into?

-Madrius-

QUOTE (Madrius @ Nov 5 2008, 08:09 AM)
Wouldn't it be simpler to produce the fusion protein yourself, and then find an inducible vector to put it into?


I have the opportunity to buy a kit, a one-step procedure is prefered

-The Bearer-

QUOTE (The Bearer @ Nov 5 2008, 12:50 PM)
QUOTE (Madrius @ Nov 5 2008, 08:09 AM)
Wouldn't it be simpler to produce the fusion protein yourself, and then find an inducible vector to put it into?


I have the opportunity to buy a kit, a one-step procedure is prefered

Look at this website. I'm not sure if this will do for what you want but it's the closest I could find.

www.qbiogene.com/products/gene-expression/qmateCymR1.shtml


-rkay447-