identifying genomic DNA contamination during plasmid isolation - (Sep/21/2007 )
i m isolating BAC vector using alkaline lysis method. how to identify whether any genomic DNA contamination in my preparation.
THank You
-nithyachary-
wel it can not be runned on standard gel electrophoresis.
what may you suspect genomic dna contamination ?
normally extra care avoid this
-fred_33-
What is the size of your BAC? Can you do a restriction digest and check/resolve the resulting fragments
If you are working on really large BAC (>100kb), you can do a pulse field gel electrophoresis. That should resolve your BAC.
A few details would be helpful.
-perneseblue-