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identifying genomic DNA contamination during plasmid isolation - (Sep/21/2007 )

i m isolating BAC vector using alkaline lysis method. how to identify whether any genomic DNA contamination in my preparation. unsure.gif
THank You

-nithyachary-

wel it can not be runned on standard gel electrophoresis.
what may you suspect genomic dna contamination ?
normally extra care avoid this

-fred_33-

What is the size of your BAC? Can you do a restriction digest and check/resolve the resulting fragments

If you are working on really large BAC (>100kb), you can do a pulse field gel electrophoresis. That should resolve your BAC.

A few details would be helpful.

-perneseblue-