How to spot plasmid on paper for shipping? - (Feb/09/2006 )

Hi all,

I need to send out a plasmid (to one of our lab's collaborators) by blotting on filter paper. Can somebody tell me the exact procedure for doing it: what's the amount of DNA to be spotted, which paper to use, how long to let it sit, etc. Thanks.

Manoj.

-manoj-

Hi Manoj,

You can just spot your plasmid on a piece of filter paper. Before spotting, you can draw a circle on the paper using a pencil so that the recepitent will know where it is. There is no real limit on the amount of plasmid you can spot, it all depends on the concentration of your DNA.

-pcrman-

So do you soak the piece of filter paper in comp cells and transform or what?

I've heard of this technique before but I have not tried it.

Regards,

Matt

-MisticMatt-

QUOTE (MisticMatt @ Feb 10 2006, 04:27 PM)

So do you soak the piece of filter paper in comp cells and transform or what?

I've heard of this technique before but I have not tried it.

Regards,

Matt

you soak the piece of filter paper in TE (I take a sterile tip and smush around a little) then take say 5-10ul and transform...

-beccaf22-

hey, Matt, if you are looking for some technical references, this is commercially available as a product called Clonesaver

-aimikins-