Do I need to clone into gateway vector or pGEMT easy vector before cloning into - (Feb/11/2008 )

For cloning the UTR into pGL3 vector, I always cloned into pGEMT first before cloning into pGL3 vector. i found it is very efficient to do this way.


Do I need to clone into gateway vector or pGEMT easy vector before cloning into GST tagged vector?

some people used the Topo cloning vector (gateway vector) before cloning into pcDNA3.1, I have become used to using pGEMT easy vector, is it ok for me to use pGEMT easy vector before cloning into pcDNA3.1?

You could actually clone directly into the pcDNA 3.1. This is what we always do in our lab.