Vector question - (May/21/2006 )

Hello,

In my vector, there is for the MCS, a beta-globin intron located. Does anybody know what the reason of this intron is?

I didnt understand... is the MCS a b globin intron? I would be happy enough knowing that my MCS has enough restrictions sites for my clonings

My vector starts with the SV40 ori, after the ori, there is a beta-globin intron located, after this intron is the MCS located, and after is, there is a PRE gene (post-transcriptional regulatory-elements) and the poly-A signal located.

My question is what the reason is of the beta-globin intron, i think it is something like a enhancer for the expression of my gene product. But I can't find anything with google...

Sound to me like spacer sequence with no real function. It creates physical distance between the ori & mcs.

hi
B globin intron is here to be sure your RNA will be processed by splicing. Some proteins are better expressed (or are just "expressed) if the pre mRNA is spliced.

I have heard people claim in conferences that intron helps in 2 - 4 x better expression. I didnt find any difference when I tried expressing EGFP with and without intron. May b like fred wrote, it is only for some proteins.

Thank you for your help, i had red some articels that told the same you did, but I did not know it for sure.