T1 phage infection - can it be due to poor lab practice? (Jun/04/2008 )
Every spring/summer we get a phage outbreak in our lab.
I am a bit annoyed at the moment as I warned of people leaving autoclaved cultures hanging about in the lab for days and days.
T1 phage is very resistant to cleaning methods, and I argued that we are giving them a foothold through poor lab practice.
Does anyone agree or am I wrong and this had nothing to do with phage outbreaks? I should say that we use T1 resistant strains of E.coli, but it is not that uncommon to still get phage infection according to experts I have spoken to.
Any literature links would be appreciated!
GK
UC Santa Cruz
-grahamkeith-
QUOTE (grahamkeith @ Jun 4 2008, 05:36 AM)
Every spring/summer we get a phage outbreak in our lab.
I am a bit annoyed at the moment as I warned of people leaving autoclaved cultures hanging about in the lab for days and days.
T1 phage is very resistant to cleaning methods, and I argued that we are giving them a foothold through poor lab practice.
Does anyone agree or am I wrong and this had nothing to do with phage outbreaks? I should say that we use T1 resistant strains of E.coli, but it is not that uncommon to still get phage infection according to experts I have spoken to.
Any literature links would be appreciated!
GK
UC Santa Cruz
I am a bit annoyed at the moment as I warned of people leaving autoclaved cultures hanging about in the lab for days and days.
T1 phage is very resistant to cleaning methods, and I argued that we are giving them a foothold through poor lab practice.
Does anyone agree or am I wrong and this had nothing to do with phage outbreaks? I should say that we use T1 resistant strains of E.coli, but it is not that uncommon to still get phage infection according to experts I have spoken to.
Any literature links would be appreciated!
GK
UC Santa Cruz
frankly, this is the first time I have heard of T1 phage outbreaks! I would love to know more about this wonderful thing that I missed most of my research life.
-cellcounter-
http://en.wikipedia.org/wiki/Bacteriophage
fill your boots
-grahamkeith-
QUOTE (grahamkeith @ Jun 6 2008, 04:37 AM)
QUOTE
Every spring/summer we get a phage outbreak in our lab.
I was refering to "knowing more about phage outbreak in your lab". I wrote 95% of that wikipedia article.
-cellcounter-
QUOTE (cellcounter @ Jun 6 2008, 08:26 AM)
QUOTE (grahamkeith @ Jun 6 2008, 04:37 AM)
QUOTE
Every spring/summer we get a phage outbreak in our lab.
I was refering to "knowing more about phage outbreak in your lab". I wrote 95% of that wikipedia article.oh, right, haha. Well, yes, it appears to be seasonal with us! Every year for the past four years it happens at around the same time. Why this is, noone knows. Some guy from Microbiology even managed to sequence a few of them! Other than that, I don't know. I will ask about for more details.
-grahamkeith-