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Plasmid DNA concentration - (Nov/17/2008 )

Hi there

Is it possible to liofilize DNA in order to concentrate your plasmid DNA samples?

Thanks

-Lmaso-

QUOTE (Lmaso @ Nov 18 2008, 09:51 AM)
Hi there

Is it possible to liofilize DNA in order to concentrate your plasmid DNA samples?

Thanks

Sure, but remember that any salts will be concentrated, unless they are volatile. Why not just EtOH precipitate and resuspend in a smaller volume? Or you could reduce the volume by extraction with butanol (also concentrates salts, though).

-swanny-

Thanks... it worked just fine wink.gif

I had my plasmid DNA in water, so there was no problem with salt concentration.

-Lmaso-