Plasmid DNA concentration - (Nov/17/2008 )
Hi there
Is it possible to liofilize DNA in order to concentrate your plasmid DNA samples?
Thanks
-Lmaso-
QUOTE (Lmaso @ Nov 18 2008, 09:51 AM)
Hi there
Is it possible to liofilize DNA in order to concentrate your plasmid DNA samples?
Thanks
Is it possible to liofilize DNA in order to concentrate your plasmid DNA samples?
Thanks
Sure, but remember that any salts will be concentrated, unless they are volatile. Why not just EtOH precipitate and resuspend in a smaller volume? Or you could reduce the volume by extraction with butanol (also concentrates salts, though).
-swanny-
Thanks... it worked just fine
I had my plasmid DNA in water, so there was no problem with salt concentration.
-Lmaso-