non dephosphorylated vector gave white colonies - (Oct/22/2008 )

Hi there,

I've prepared a dephosphorylated BamHI digested vector. I've done the transformation and I've got a lot of white colonies in the control with the non dephosphorylated vector. I expected a lot of blue colonies... Somebody know how's that possible?

Best regards

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Do you mean that you used a non dephosphorylated vector and a dephosphorylated vector to do the ligation?
And then the non dephosphorylated construct is gone into your cells after transfection?
So, I never dephosphorylate my vectors and I have got a really good transfection efficiency, it isn't necessary!

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maybe they just need some X-gal and IPTG

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