How to make plasmid map? - (Oct/11/2006 )

Hi,

Is there any online program which can be used to make plasmid maps? This morning I tried using plasmapper but it doesn't have any option for cutting the vector and insert with restriction enzymes and then putting them together to make a complete plasmid map (vector+insert).

For e.g. Vector: pSP73 cut with BglII-XhoI and
Insert: Interferon-g cut with BglII-XhoI

Could you please tell me how do I make a plasmid map for the above construct? I mean using some online free software.

Thanks.

-exploresci-

u may try vector NTI from invitrogen.

http://www.invitrogen.com/content.cfm?page...=FL-VNTIGATEWAY

-dodosko-

VEctor NTI works quite nicely. May take some time getting used to it.

-scolix-

Yes its free, When I do my plasmid maps I cut and paste manually the sequences in the insertion sites. I never try clonning functions in Vector NTI.

Best.

-djv0022-

BioEdit does a nice job also.

http://www.mbio.ncsu.edu/BioEdit/bioedit.html

-Crystalguy-