Endogenous/Native Plasmid Isolation - (Apr/19/2007 )

Hey have anyone isolated endogenous/native plasmids from wild gram negative bacterial strains? If anyone knows how to go about it or if you know a protocol exists, or if if you know the methods to identify endogenous plasmids, please discuss. itz an interesting topic

There are several methods for this, some of which are designed for megaplasmids:

Take look here:

plasmid isolation 1

and here:

plasmid isolation2

and this great review:

plasmid review

Hope these help!

~ Dave