Vector databases and resources - (Nov/23/2005 )

Vector and plasmid databases

NCBI Entrez Nucleotide Database: You can search for vector sequence by vector name

• List of Vectors from Univec

• Bacterial expression vectors
• Insect cell expression vectors
• GATEWAY cloning and expression vectors
• Cloning and auxiliary vectors

Hi,

I'm looking for expression vector to introduce specific gene in mammalian cells in vitro.

Is there commercially available vector that contain Ig heavy chain promoter and light chain enhancer???

Dear scientist,

Addgene is a non-profit plasmid repository dedicated to helping scientists archive and share their published plasmids. We have collected a significant number of plasmids from individual laboratories, and have made them available through our website, www.addgene.org .

Because Addgene provides plasmid cloning information along with the physical samples, we have also compiled a database of commonly used vector backbones created by companies and labs. To search the database, visit:

www.addgene.org/vectors

We welcome you to deposit your lab's plasmids at Addgene and we are interested in hearing your feedback.

Best wishes,
Melina Fan

**********
Melina Fan, PhD
Addgene
One Kendall Square
Building 600, 3rd Floor
Cambridge, MA 02139
(617)225-9000

info@addgene.org
www.addgene.org

BioInfoMan (http://bioinfoman.com/bm/pcs.php) has over 5000 commonly used constructs.

The links for EMBL does not work, I get this message

Do any of these databases allow one to search by restriction enzyme(s)? I am working on some cloning projects and would like to find a way to search a collection of plasmids for REs without having to look at every MCS for every plasmid offered by every vendor.

Thanks for your help.

Justin

Is there anybody who can give some suggestion to the above question?
Thanks very much.
Jeff