691. Cell Surface Biotinylation - Advantage/Disadvantage (reply: 7)
692. Temperature effect of protein? - (reply: 5)
693. Histone substrates for in vitro kinase assay - I need to order Histone H1/H3 to do kinase assay. (reply: 2)
694. How to backtrack to DNA sequence from protein - (reply: 4)
695. About Tween 20? - (reply: 2)
696. Protein loading control? - (reply: 4)
697. How to express toxic protein in BL21(DE3) or (pLysE) strain - (reply: 1)
698. 2D Gel Electrophoresis Software - (reply: 1)
699. Purification of HisTag Recombinant Protein - Advices needed (reply: 2)
700. how can I get enough proteins for Mass spec? drive me mad! - (reply: 12)
701. Antibody storage - (reply: 4)
702. How to disolve Chloromphenicol - (reply: 4)
703. minimum amount of cells used for western blot - (reply: 5)
704. cathepsin C detection in Western blot - (reply: 1)
705. Linker design - Design of linker to fusion two protein domains (reply: 1)
706. 3D protein structure - (reply: 2)
707. TRIS - (reply: 1)
708. SDS-PAGE problems: proteins not running entire legnth - (reply: 7)
709. SDS Gel - (reply: 4)
710. Antibodies and triton X - (reply: 4)
711. What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
712. RNA polymerase a sa fractionation control? - (reply: 3)
713. Does this result indicates enhanced proteasomal gedradation? - (reply: 7)
714. Setting tris-glycine gels and storing them BEFORE running them? - (reply: 7)
715. His-tag: C-terminal cleavable through protease? - (reply: 1)
716. COX-2 positve control for western blotting? - COX-2 positive control for western blotting? (reply: 3)
717. protein purification problem - (reply: 3)
718. lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
719. Protein expression from bacteria which has two plasmids? - Incompatibility of plasmids (reply: 5)
720. Calculating protein concentration - (reply: 5)

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