2011. GST protein purification from inclusion bodies... - will GST part still be intact? (reply: 2)
2012. Is this possible with HPLC? - chech the purity (reply: 1)
2013. can I freeze my samples in loading buffer and use them the next day? - (reply: 2)
2014. Suicide substrate system - (reply: 5)
2015. Troubleshoot Chocked Ni-NTA Column - (reply: 2)
2016. peptidase cleavage sites - (reply: 3)
2017. Phosphorylated proteins - (reply: 1)
2018. Protein behavior at high concentration - (reply: 4)
2019. Whole cell vs. supernatant - (reply: 4)
2020. problem of molecular weight - (reply: 3)
2021. Are DNase inhibitors available? - help (reply: 3)
2022. How can I remove the Polyhistindine tag or myc epitope from my recombinant prote - (reply: 3)
2023. isolating phospho-proteins - After stimulating the pathway with EGF, can we still trypsinise or do we have to (reply: 1)
2024. Besides high temp, how to reverse formaldehyde cross-linking ? - (reply: 1)
2025. His tagged protein from mammalian cells - purification of His tagged (reply: 1)
2026. BRADFORD reagent - (reply: 4)
2027. Cell Lysates for western - (reply: 4)
2028. Normalizing Western Blot to cell number without cell counting - (reply: 5)
2029. EMSA problems - (reply: 3)
2030. Samples wont move? - (reply: 3)
2031. QuantityOne software - Western Blot (reply: 1)
2032. purification problem. - most of my protein is washed away.... (reply: 10)
2033. Protease purification - plant leaf protease (reply: 1)
2034. SDS-PAGE for A-beta proteins from cell lysate - (reply: 6)
2035. Anyone has experience to grow sespension 293 cells? - (reply: 1)
2036. supershift-EMSA problem - (reply: 3)
2037. faint ladder on my membrane. Can hardly see it! - (reply: 9)
2038. Question about synthesis of serine - (reply: 1)
2039. In vitro transcription/translation with pDest14 - (reply: 1)
2040. BN/SDS 2D-PAGE - (reply: 1)

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