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1741. how to purify fusion proteins on a large scale? - (reply: 2)
1742. Problem in choosing internal contol for Western blot - not actin, tubulin, GAPDH and VDAC (reply: 11)
1743. The biology activity of espressed protein - (reply: 3)
1744. how to prepare total cell lysate for Western blot? - (reply: 10)
1745. separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
1746. Immunoprecipitation - is crosslinking neccesary? - (reply: 7)
1747. how can I increase protein expression level - hurry (reply: 2)
1748. Cyclic peptides multimerize! - How to proceed? (reply: 6)
1749. what is the meaning of de -gas the acrylamide gel mix? - (reply: 6)
1750. Imidazole + enzyme activity - (reply: 6)
1751. SDS-PAGE gel preparation - (reply: 10)
1752. Western band distorted by 'bubbles' - (reply: 8)
1753. western blotting membranes - (reply: 6)
1754. Extinction coefficient - (reply: 2)
1755. Western blot ECL quantification without previous Bradford/Lowry assay... - (reply: 3)
1756. N-ethylmaleimide handling - (reply: 5)
1757. Extra band in western blot - (reply: 1)
1758. concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
1759. T-protein reducing faster than its p-form - any idea why?? (reply: 1)
1760. Protein determination - Old Skool style - I'm looking for a decent descriptive protocol for protein detection using th (reply: 6)
1761. ERK by Westerns - (reply: 8)
1762. Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
1763. in vitro translation... - why not? (reply: 3)
1764. Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
1765. quantification of WB bands- how to get similar values from different experiments - (reply: 4)
1766. how to do western with 500 kD protein? - (reply: 5)
1767. DsRed fusion protein not glowing - It is there by WB though (reply: 3)
1768. OD and protein - calculation (reply: 2)
1769. Blocking Western with Fab fragment - (reply: 3)
1770. Western blot bands fade out - (reply: 9)
1742. Problem in choosing internal contol for Western blot - not actin, tubulin, GAPDH and VDAC (reply: 11)
1743. The biology activity of espressed protein - (reply: 3)
1744. how to prepare total cell lysate for Western blot? - (reply: 10)
1745. separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
1746. Immunoprecipitation - is crosslinking neccesary? - (reply: 7)
1747. how can I increase protein expression level - hurry (reply: 2)
1748. Cyclic peptides multimerize! - How to proceed? (reply: 6)
1749. what is the meaning of de -gas the acrylamide gel mix? - (reply: 6)
1750. Imidazole + enzyme activity - (reply: 6)
1751. SDS-PAGE gel preparation - (reply: 10)
1752. Western band distorted by 'bubbles' - (reply: 8)
1753. western blotting membranes - (reply: 6)
1754. Extinction coefficient - (reply: 2)
1755. Western blot ECL quantification without previous Bradford/Lowry assay... - (reply: 3)
1756. N-ethylmaleimide handling - (reply: 5)
1757. Extra band in western blot - (reply: 1)
1758. concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
1759. T-protein reducing faster than its p-form - any idea why?? (reply: 1)
1760. Protein determination - Old Skool style - I'm looking for a decent descriptive protocol for protein detection using th (reply: 6)
1761. ERK by Westerns - (reply: 8)
1762. Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
1763. in vitro translation... - why not? (reply: 3)
1764. Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
1765. quantification of WB bands- how to get similar values from different experiments - (reply: 4)
1766. how to do western with 500 kD protein? - (reply: 5)
1767. DsRed fusion protein not glowing - It is there by WB though (reply: 3)
1768. OD and protein - calculation (reply: 2)
1769. Blocking Western with Fab fragment - (reply: 3)
1770. Western blot bands fade out - (reply: 9)
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