1891. Protein Lysate and blotting problems - (reply: 2)
1892. transformation into pichia pastoris - no transformants with pPICZAalpha (reply: 4)
1893. Western blotting without transfering protein to membrane - (reply: 4)
1894. eluting protein from the gel band... - (reply: 7)
1895. Antibody Vs. silver - (reply: 1)
1896. SDS-PAGE: transfer/blotting difficulty - (reply: 4)
1897. How to study the expression of one, specific gene? - (reply: 5)
1898. aggregation of purified recombinant protein on dialysis-what do to? - (reply: 1)
1899. dye front running funny - western blot (reply: 3)
1900. so my protein has degraded....why am I seeing higher bands? - (reply: 3)
1901. PVP: Polyvinylpyrollidone - protein extraction buffer (reply: 3)
1902. Help needed-Protein extraction from native gel - (reply: 3)
1903. gel shift assay help- big smear lane - (reply: 2)
1904. Y2H vectors compatible? - (reply: 1)
1905. strange western - transfer turned PVDF, filter, sponge, and buffer yellow? (reply: 6)
1906. Western AB unselective or posttranslational modifications? - (reply: 1)
1907. so tired of this protein... - another strain of E-coli? (reply: 33)
1908. Information on GIGApite - (reply: 1)
1909. (HELP)Properties of protein after glycosylation - any effect on pI n etc? (reply: 2)
1910. induction with IPTG ? - (reply: 3)
1911. Eliza to work out linear response - (reply: 1)
1912. What is the name of substances that covalently binds proteins - (reply: 1)
1913. method of making nuclear extract for protein interaction? - (reply: 1)
1914. how to calculate pmole ends? - (reply: 1)
1915. What do you do with YOUR membranes? - (reply: 7)
1916. Protein Half-life determination - (reply: 1)
1917. problems with expression of mutant protein - (reply: 1)
1918. Western Blot - fixation of proteins with glutaraldehyde? - (reply: 4)
1919. I have problem on Actin band - SDS-PAGE and Western blot (reply: 3)
1920. TCA vs. meth/chloro - concentration of proteins (reply: 3)

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