1591. Protein Phosphorylation - (reply: 1)
1592. Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
1593. stripped memberane can be used for phosphprotein detection? - (reply: 6)
1594. folding of IL2 - (reply: 2)
1595. Burned membrane?.... - (reply: 1)
1596. problem of protein expression - problem of protein expression (reply: 1)
1597. EMSA -- Chemiluminescent version by Pierce - (reply: 1)
1598. why some antibodies need overnight incubation for Western blot - (reply: 4)
1599. Percentage of SDS- PAGE gel for co-IP - (reply: 1)
1600. Protein extraction-what's in the pellet? - (reply: 6)
1601. Protein Transfection by Electroporation - (reply: 6)
1602. Lost: protein. Last seen prior to purification. - not present in flow through, washes or elutions! (reply: 2)
1603. Western blotting for ER - problems - (reply: 7)
1604. sds-page of hemocyte lysate proteins - (reply: 2)
1605. Coomassie G-250 vs R-250 - (reply: 3)
1606. 3T3 cells and lysis buffer recepie - western blot (reply: 1)
1607. silver staining trouble - (reply: 3)
1608. Precepitation during dialysis - (reply: 6)
1609. 2D Gel electrophoresis analysis software - Is a good one free available? (reply: 1)
1610. Storing PVDF membranes for N-terminal sequencing - Urgent! (reply: 1)
1611. gangliosides - (reply: 4)
1612. Does glycerol intefere an enzymatic reaction ? - (reply: 4)
1613. GST-tagged protein purification. - inclusion body formation of my GST taggged protein. (reply: 3)
1614. Question about the strategy of protein purification - (reply: 4)
1615. How to detect the protein concentration by UV light - (reply: 2)
1616. protein dialysis - (reply: 1)
1617. anti GFP - IP - (reply: 3)
1618. Immunoprecipitation-I can't see the heavy and light chain bands - (reply: 1)
1619. Why should add DTT and what kind protein assay I should use - (reply: 4)
1620. Problem with protein refolding - protein precipitate all the time !!! why (reply: 2)

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