421. How to prepare membranes from E.coli? - (reply: 2)
422. Collagen I and V western blot - Blue Native PAGE - Blue Native PAGE (reply: 7)
423. Cleaning Up an Immunoprecipitation - (reply: 2)
424. Native gel and salt - (reply: 2)
425. Big problem in GST Protein purification - (reply: 9)
426. preferred method for purifying antibodies - (reply: 2)
427. agents to break hydrophobic interactions.. - how to distinguish disulphide vs hydrophobic protein protein interacti (reply: 1)
428. DTT vs. Beta Mercaptoethanol.. which is a stronger reducing agent? - what are the optimum conditions for each? thnks! (reply: 4)
429. Bradford assay - (reply: 2)
430. please have your say on lysis/lysozyme inaction - (reply: 5)
431. protein purification - (reply: 5)
432. preparing samples for phosphorylation sites identification - (reply: 3)
433. Time for electrotransfer, methanol on PVDF - (reply: 2)
434. Trying to get protein out of insoluble fraction - (reply: 3)
435. Membrane protein extraction from mice tissue - (reply: 3)
436. IP band slightly different size than input - (reply: 4)
437. antibodies testing - (reply: 4)
438. protein expression in NSO cells - (reply: 6)
439. solublization of basic protein - (reply: 3)
440. What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
441. what's the function of glycerol in co-ip buffer - (reply: 2)
442. Surface Plasmon Resonance - Molecule immobilization (reply: 3)
443. secretome profile - (reply: 3)
444. Is TCA really specific in precipitating proteins from cell lysate? - (reply: 1)
445. Protein reversed phase analysis.. linear or step gradients? - which is preferred and why?? (reply: 3)
446. Why HEPES and folic acid in my lysis buffer? - (reply: 4)
447. Cell Lysis - (reply: 2)
448. protein isolation from cell lines - membrane bound proteins (reply: 3)
449. protein extraction - (reply: 1)
450. disappear protein band after development - (reply: 11)

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