Protein loading control? - (Aug/08/2007 )

What are the most usual protein loading controls for Western blots found in papers about bacteria ? I need something that can be purchased.

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For example Rainbow Mw protein markers ( GE Healthcare)?

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But that is a marker. I am looking for a loading control that can be loaded in every lane so that I can monitor how correct my loading is.

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You will have to measure the amount of protien in your samples and load the same amounts and you will have to detect expression of a housekeeping gene using antibodies to show equal loading of each lanes.

in mammalian cells, one might use actin for control.

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Yes, you need a housekeeping gene for a loading control. For example, I use either hsp90, actin, or GADPH as loading controls against mammalian cell proteins. Just remember to choose one of a size that is considerably far from the target protein.

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