2641. GST purification - general protocol (reply: 2)
2642. protein isolation from brain - (reply: 1)
2643. how to apply molecular weight marker on 2D gels? - application marker for 2D (reply: 1)
2644. "Westerning" your self out... - (reply: 7)
2645. GFP protein not soluble? - (reply: 1)
2646. Larger than expected bands on a Western - What could they be? (reply: 2)
2647. small protein expression in yeast - expression of an 8kD his tagged protein in yeast (reply: 1)
2648. co-IP problem - (reply: 1)
2649. Protein hydrophobicity - (reply: 1)
2650. good transfer? how to determine? - (reply: 3)
2651. WB for Membrane Protein - Not able to see membrane protein on western (reply: 12)
2652. Trypsin vs Non Enzymatic - (reply: 2)
2653. transfer overnight? - (reply: 6)
2654. Expressing protein in a soluble form - What should be the next logical step? (reply: 8)
2655. EMSA/Gel Shift wells problem - Protein stuck in the wells of EMSA gel (reply: 10)
2656. anyone has idea to raise antibody, how much protein need? - (reply: 1)
2657. Non-specific binding in binding assay - BSA blocking step (reply: 6)
2658. alternative to bromophenol? - (reply: 7)
2659. Chemilluminscence issues - (reply: 7)
2660. please direct me to a good general IP protocol - looking for something that works! (reply: 1)
2661. Dry SDS-PAGE gels - methods, tips to safely dry protein gels (reply: 2)
2662. His-tag protein purificaton, cotamination with 40-43kD protein - (reply: 1)
2663. application of co-IP - (reply: 5)
2664. MBP tagged protein not bind resin - (reply: 8)
2665. IP FLAG Phusion protein - Lysis conditions (reply: 2)
2666. Protein Expression Analysis Question - Protein Expression Analysis (reply: 1)
2667. cDNA size VS protein Size - MW weight confusion (reply: 7)
2668. Enterokinase cleavage problem - HisTag (reply: 1)
2669. Protein Solubilisation - Dissolving tofu (reply: 4)
2670. Lysis buffers for brain tissue samples - Which ingredients are superfluous? (reply: 3)

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