931. protein-protein interaction - (reply: 3)
932. MW marker for 150 kDa protein in Western blot - (reply: 5)
933. how to check protein-primary antibody binding - Is there any method or technique to check or proof read the primary Ab (reply: 7)
934. Stringent wash buffer for Immunoprecipitation - What decides stringency of wash buffer when perform IP? (reply: 2)
935. Tricine gel storage - can i store fully made gels overnight? (reply: 6)
936. About rasmol - (reply: 2)
937. HIS tag elution woes.. - (reply: 3)
938. Purifying His-Tagged Proteins from Insects cells on IMAC resin? - (reply: 1)
939. Correcting phospho-protein with total protein - (reply: 2)
940. SDS page - SDS page running strangely (reply: 5)
941. Protein insoluble in 8M urea or 6MGuHCl - (reply: 8)
942. promlems in protein purification in native form - (reply: 2)
943. Best lysis buffer for mammalian cells to do affinity chromatography? - (reply: 3)
944. purification of proteins from sample - (reply: 12)
945. how can I dialyze protein through its PI and avoid precipitation? - (reply: 1)
946. western block milk - milky colour disappears after 1 hour of blocking (reply: 12)
947. Humid and semi-dry Western blot - (reply: 1)
948. Western blot membrance blocking and storage - (reply: 4)
949. where I can order a synthesized peptide? - synthesized peptide (reply: 2)
950. What's good and bad about C-tem and N-term His tag? - (reply: 3)
951. Problem with Desalting-Column - Protein gets lost while Desalting (reply: 2)
952. peotein Structural variation - ..plz help (reply: 2)
953. Immunoprecipitation & Mass Spec analysis - How much mg(ug) of protein lysate is needed for IP followed by Mass Sp (reply: 2)
954. What buffer should I incubate HRP secondary? - (reply: 3)
955. Is 20% methanol sufficient to fix protein in gel? - (reply: 5)
956. mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
957. Weak and variable signal blotting for MDR1 using C219 monoclonal Ab - (reply: 2)
958. Unable to identify our protein - need an idea (reply: 18)
959. Co-immunoprecipitation - antigen elution without the antibody - (reply: 4)
960. electrophoresis question - (reply: 6)

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