2821. Concentration of secreted protein in culture media - Concentration of culture media (reply: 7)
2822. gel running problems - lanes are smeared lines on the gel (reply: 3)
2823. can i freeze cell lysates for western blotting? - (reply: 3)
2824. Destain time - (reply: 2)
2825. IP - losing proteins from protein G beads - (reply: 1)
2826. question - anti Ha western blotting (reply: 1)
2827. phosphorylation experiment without using radiation? - (reply: 8)
2828. Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
2829. IEF followed by Western blot - problems with poor blotting efficiency (reply: 4)
2830. Protein extraction from solid matrix - (reply: 1)
2831. PVDF question - (reply: 3)
2832. Protein sticking to membrane - Protein concentration woes (reply: 1)
2833. expression of fusion protein - how to lysis membrane protein (reply: 2)
2834. which company sell small volumn column? - (reply: 6)
2835. His-tagged protein binding problem - Protein Binding (reply: 1)
2836. Is my gel not polymerising fully and why......? - (reply: 1)
2837. lysis buffer for western blot - lysis buffer (reply: 3)
2838. his tag - his tag purification (reply: 2)
2839. Size Exclusion Column - Proteins keep sticking!! - (reply: 2)
2840. a recipe of a lysis buffer for co-IP (phosphorylation) - can anyone recommend one? (reply: 3)
2841. what these chemicals for in lysis buffer? - (reply: 3)
2842. activity loss - (reply: 1)
2843. Western Blotting - Electrophoretic transfer of proteins (reply: 2)
2844. phospho-(Ser) PKC substrate antibody - (reply: 1)
2845. membrane and mitochondrial protein fractionation - (reply: 1)
2846. Needs the protocols - (reply: 1)
2847. BCA assay - (reply: 2)
2848. gel staining - (reply: 1)
2849. improve pritein expression - (reply: 6)
2850. separate proteins from sucrose - (reply: 3)

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