721. 3D protein structure - (reply: 2)
722. TRIS - (reply: 1)
723. SDS-PAGE problems: proteins not running entire legnth - (reply: 7)
724. SDS Gel - (reply: 4)
725. Antibodies and triton X - (reply: 4)
726. What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
727. RNA polymerase a sa fractionation control? - (reply: 3)
728. Does this result indicates enhanced proteasomal gedradation? - (reply: 7)
729. Setting tris-glycine gels and storing them BEFORE running them? - (reply: 7)
730. His-tag: C-terminal cleavable through protease? - (reply: 1)
731. COX-2 positve control for western blotting? - COX-2 positive control for western blotting? (reply: 3)
732. protein purification problem - (reply: 3)
733. lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
734. Protein expression from bacteria which has two plasmids? - Incompatibility of plasmids (reply: 5)
735. Calculating protein concentration - (reply: 5)
736. tips for detect phosphorylated proteins - (reply: 2)
737. Protein not entering inclusion bodies - (reply: 3)
738. Stability of primary antibodies at room temperature - (reply: 4)
739. westerns, protein concentr and RIPA buffer - (reply: 2)
740. 2D AND DIGE - (reply: 1)
741. Western Blots with (250 kDa) High Molecular Weight Protein - (reply: 2)
742. total protein assay before western blot - (reply: 1)
743. western blot for nuclear and cytoplasm seperation sample - (reply: 3)
744. Trouble finding 5kDa protein in western blot - (reply: 5)
745. kinase assay with GST-fused kinase and histone H1 as a substrate - can I try kinas assay using non-radiolabeled ATP? (reply: 1)
746. Imidazole and hydrophobic interation chromatography - (reply: 1)
747. how to remove PEG in my protein sample - (reply: 1)
748. Why 2 bands for housekeeping? - western blot (reply: 2)
749. Larger sized Protein Standard Ladder Req - (reply: 1)
750. GST pulldown assay problem - (reply: 2)

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