3061. How resuspend protein after (NH4)2SO4 precipitation? - (reply: 2)
3062. Western blot problem: not running in gel - western blot troble (reply: 7)
3063. Problem with Hsp72 - (reply: 2)
3064. losing protein activity - (reply: 3)
3065. His-tag protein purification - contamination with other... - ...His-rich proteins (reply: 15)
3066. Help: baculovirus expression system? Anyone's used it? - Has anyone used this system (reply: 1)
3067. Nonspecific Binding on Western Blot - HELP (reply: 10)
3068. western blotting question - (reply: 3)
3069. Extraction of membrane proteins and western on them - (reply: 3)
3070. Proteins in sample buffer - Interference with protein assays (reply: 1)
3071. Precipitation with TCA and Na deoxycholate - what is the role of deoxycholate? (reply: 4)
3072. I forgot pretreating the dialysis membrane ! - (reply: 1)
3073. expression problems? - (reply: 3)
3074. Anybody knows any resolutions to improve TAP purification? - (reply: 1)
3075. centrifuging - protein extraction (reply: 4)
3076. Western Blotting Spotting - Spots on Westerns Blots (reply: 5)
3077. expression of small peptide - how to identify the small peptide? (reply: 2)
3078. ion-exchange chrom. under denature conditions - purification on IEX chrom from inclution body (reply: 2)
3079. MAPK P38 - troubles with western blot (reply: 8)
3080. EMSA/ band shift enquiries - EMSA/ band shift enquiries (reply: 1)
3081. Imidazole problem - (reply: 3)
3082. Problem with his tag protein purification - (reply: 2)
3083. protein purification - his-tag protein purification (reply: 2)
3084. gst-fusion protein, mainly - recombinant protein (reply: 2)
3085. choosing insect cell for high-cystine protein expression - (reply: 1)
3086. Flag-tag protein - Need advice for Flag-tag protein (reply: 1)
3087. SDS page running strangely - (reply: 1)
3088. serine/threonine phosphatase inhibitors - what are the commonly used ones (reply: 5)
3089. chromatograhy! using acetone... - (reply: 1)
3090. The elution volume (Ve) - (reply: 1)

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