811. EMSA-Difficulties - no clean lanes (reply: 1)
812. iodoacetamide prep - (reply: 2)
813. WB: protein amount/ lane ? - What is the optimal protein amount per lane in WB ? (reply: 6)
814. Protein purification - (reply: 2)
815. protein stability issues - (reply: 4)
816. Spot excision machine for PAGE - (reply: 3)
817. 40kDa protein for ladder - (reply: 4)
818. gus or gfp - gus or gfp (reply: 2)
819. How to extract protein from a bone marrow sample - protein from bone marrow (reply: 1)
820. loading control for nuclear protein in rabbit cell extract - (reply: 1)
821. Western blotting and immunofluorescence. Results don't match. - This is madness... (reply: 7)
822. Why does my peptide run above its size after incubation with plasma - (reply: 1)
823. NP40 lysis buffer - (reply: 3)
824. inclusion bodies? - (reply: 7)
825. samples floating out of SDS-page gel well immediately after loading - (reply: 7)
826. Rainbow MW marker from Amersham - detection with a mouse secondary antibody? (reply: 1)
827. Protein Extraction from cell media for western blot - (reply: 3)
828. Immunoprecipitation of a secreted protein - (reply: 5)
829. Expect monomer on SDS PAGE gel get dimer or larger - (reply: 3)
830. N-terminal protein sequencing.. "holes" at lysine residues.. meaning? - what kinda modifications cud've happnd? (reply: 2)
831. chlorophyll - (reply: 1)
832. using IgM antibodies in IP - (reply: 3)
833. Western Blot for Higher molecular weight Proteins - (reply: 1)
834. Western problem : no signal - could that be linked to pH? (reply: 2)
835. how to reduce unspecific binding of proteins to the beads.. - IP-purification (reply: 3)
836. I have a question about westernblots. Please help - (reply: 4)
837. HELP! contaminants do not separate from my protein during purification - (reply: 5)
838. control is positive, sample is negtive - (reply: 3)
839. LOADING BUFFER and UREA ? - (reply: 6)
840. EMSA using Cy3-labelled probe - (reply: 2)

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