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2731. What is the function of NaCl in ? - (reply: 3)
2732. Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
2733. Big problem with Western Blot - Ugly blot (reply: 2)
2734. no protein expression after induction using pET vector - (reply: 4)
2735. 8M urea can't desolve the inclusion body - (reply: 2)
2736. 2D electrophoresis of Plasma - (reply: 3)
2737. Protease in expression vector - (reply: 3)
2738. Western Blots: no signal - low or no detection signal (reply: 8)
2739. Methods of proteins extraction from plants - protein extraction industry (reply: 1)
2740. His-tag degraded after purification? - (reply: 3)
2741. E.coli and inclusion bodies - growth conditions to prevent inclusion bodies (reply: 4)
2742. Help, problems with silver staining - (reply: 2)
2743. glycerol is still there after dialysis - (reply: 1)
2744. at which stage do I make glycerol stock of bacteria to express? - making glycerol stock of bacteria (reply: 10)
2745. Protein extraction from annatto fruit tissues - (reply: 3)
2746. Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
2747. dialysis of purified protein - (reply: 5)
2748. Protein isolation for western - (reply: 8)
2749. Expression even IPTG induction - (reply: 1)
2750. Reliable vendor that sells small aliquots of Antibodies? - (reply: 1)
2751. Random Background Noise for Western Blots - I'm getting noise only in some lanes (reply: 1)
2752. Native non SDS PAGE - (reply: 1)
2753. His-tagged protein co-eluted with associated protein - (reply: 4)
2754. PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
2755. extraction of extracellular protein of C. neoformans - extraction method (reply: 3)
2756. Actin Polymerization Assay...Anyone? - Native or Denatured Protein (reply: 1)
2757. His6 tag quite problematic. - Is that true? (reply: 3)
2758. protein suspended in diff buffer changes mobility - Will differnt salt concentration in buffer interfe (reply: 2)
2759. Too many unspecific bands with B-actin - Western Blot (reply: 5)
2760. Help with western blots please - (reply: 5)
2732. Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
2733. Big problem with Western Blot - Ugly blot (reply: 2)
2734. no protein expression after induction using pET vector - (reply: 4)
2735. 8M urea can't desolve the inclusion body - (reply: 2)
2736. 2D electrophoresis of Plasma - (reply: 3)
2737. Protease in expression vector - (reply: 3)
2738. Western Blots: no signal - low or no detection signal (reply: 8)
2739. Methods of proteins extraction from plants - protein extraction industry (reply: 1)
2740. His-tag degraded after purification? - (reply: 3)
2741. E.coli and inclusion bodies - growth conditions to prevent inclusion bodies (reply: 4)
2742. Help, problems with silver staining - (reply: 2)
2743. glycerol is still there after dialysis - (reply: 1)
2744. at which stage do I make glycerol stock of bacteria to express? - making glycerol stock of bacteria (reply: 10)
2745. Protein extraction from annatto fruit tissues - (reply: 3)
2746. Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
2747. dialysis of purified protein - (reply: 5)
2748. Protein isolation for western - (reply: 8)
2749. Expression even IPTG induction - (reply: 1)
2750. Reliable vendor that sells small aliquots of Antibodies? - (reply: 1)
2751. Random Background Noise for Western Blots - I'm getting noise only in some lanes (reply: 1)
2752. Native non SDS PAGE - (reply: 1)
2753. His-tagged protein co-eluted with associated protein - (reply: 4)
2754. PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
2755. extraction of extracellular protein of C. neoformans - extraction method (reply: 3)
2756. Actin Polymerization Assay...Anyone? - Native or Denatured Protein (reply: 1)
2757. His6 tag quite problematic. - Is that true? (reply: 3)
2758. protein suspended in diff buffer changes mobility - Will differnt salt concentration in buffer interfe (reply: 2)
2759. Too many unspecific bands with B-actin - Western Blot (reply: 5)
2760. Help with western blots please - (reply: 5)
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