751. identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
752. Protein in each elution step during His-Tag purification - (reply: 4)
753. GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
754. Use of urea for cell lysis - (reply: 1)
755. tag for purification... - wich one do you prefer ? (reply: 10)
756. getting rid of nonspecific background in westerns - (reply: 3)
757. problem in separating gel - (reply: 6)
758. Why different mammalian cell lysate preparation methods? - (reply: 6)
759. HA-tag - (reply: 5)
760. Limit of NaCl in the sample - (reply: 6)
761. horizontal streaking in 2-D gel? - for help (reply: 4)
762. transfer buffer question - anyone ever use TBS as a transfer buffer? (reply: 4)
763. peptide solubility - (reply: 5)
764. anti IgM secondary HRP? - (reply: 1)
765. questions about immunoprecipitation - (reply: 2)
766. Need clean HAtag antibody for mouse tissue lysates - (reply: 1)
767. isolation a protein complex - (reply: 1)
768. NADH solution decays on its own? - (reply: 1)
769. Crosslinking of oligos to membrane required for EMSA? - Is it necessary to crosslink the oligos to a positively charged nylon (reply: 2)
770. How to get rid of non-specific bands on Western blots. - (reply: 9)
771. Actin + another protein - (reply: 3)
772. How do you know if a specif Ser of the substrate protein is phosphorylated by a - (reply: 3)
773. native PAGE - (reply: 2)
774. Lack of bands in SDS-PAGE gel - (reply: 4)
775. isolation of protein complex by use percoll gradient - (reply: 1)
776. how long I can store the PAGE gles for later MALDI analysis - (reply: 1)
777. Coomassie -Papain - (reply: 3)
778. How to quantify wild-type and mutant protein? - (reply: 4)
779. milk sample in western blot - (reply: 1)
780. western blotting transfer onto membrane:why does current increase? - At constant voltage, resistance increases. The current also increases. (reply: 5)

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